7kwq - Revision history

OCA at 15:33, 18 October 2023

OCA — Wed, 18 Oct 2023 15:33:03 GMT

←Older revision		Revision as of 15:33, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='7kwq' size='340' side='right'caption='[[7kwq]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='7kwq' size='340' side='right'caption='[[7kwq]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7kwq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Vibch Vibch~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kwq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWQ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>speG, VC_A0947 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=243277 VIBCH])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diamine_N~~-~~acetyltransferase Diamine N-acetyltransferase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.3~~.1.57 2.3.1.57] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwq OCA], [https://pdbe.org/7kwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwq RCSB], [https://www.ebi.ac.uk/pdbsum/7kwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwq OCA], [https://pdbe.org/7kwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwq RCSB], [https://www.ebi.ac.uk/pdbsum/7kwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ATDA_VIBCH ATDA_VIBCH]] Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.<ref>PMID:23184347</ref> <ref>PMID:25623305</ref>	+	[https://www.uniprot.org/uniprot/ATDA_VIBCH ATDA_VIBCH] Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.<ref>PMID:23184347</ref> <ref>PMID:25623305</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7kwq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7kwq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Spermidine/spermine N-acetyltransferase\|Spermidine/spermine N-acetyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Diamine N-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibch~~]]	+	[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
-	[[Category: Boeck, P]]	+	[[Category: Boeck P]]
-	[[Category: Forwood~~, J K~~]]	+	[[Category: Forwood JK]]
-	[[Category: Gawat, D]]	+	[[Category: Gawat D]]
-	[[Category: Kuhn~~, M L~~]]	+	[[Category: Kuhn ML]]
-	[[Category: Le~~, V T.B~~]]	+	[[Category: Le VTB]]
-	[[Category: Lim~~, E Q~~]]	+	[[Category: Lim EQ]]
-	[[Category: Renolo, R]]	+	[[Category: Renolo R]]
-	[[Category: Solis, A]]	+	[[Category: Solis A]]
-	[[Category: Tsimbalyuk, S]]	+	[[Category: Tsimbalyuk S]]
-	~~[[Category: Speg enzyme allosteric]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:40, 12 May 2021

OCA — Wed, 12 May 2021 09:40:20 GMT

←Older revision		Revision as of 09:40, 12 May 2021
Line 1:		Line 1:

	==Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT==		==Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT==
-	<StructureSection load='7kwq' size='340' side='right'caption='[[7kwq]]' scene=''>	+	<StructureSection load='7kwq' size='340' side='right'caption='[[7kwq]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7KWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kwq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7kwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwq OCA], [~~http~~://pdbe.org/7kwq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7kwq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7kwq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwq ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">speG, VC_A0947 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwq OCA], [https://pdbe.org/7kwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwq RCSB], [https://www.ebi.ac.uk/pdbsum/7kwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ATDA_VIBCH ATDA_VIBCH]] Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.<ref>PMID:23184347</ref> <ref>PMID:25623305</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polyamines regulate many important biological processes including gene expression, intracellular signaling, and biofilm formation. Their intracellular concentrations are tightly regulated by polyamine transport systems and biosynthetic and catabolic pathways. Spermidine/spermine N-acetyltransferases (SSATs) are catabolic enzymes that acetylate polyamines and are critical for maintaining intracellular polyamine homeostasis. These enzymes belong to the Gcn5-related N-acetyltransferase (GNAT) superfamily and adopt a highly conserved fold found across all kingdoms of life. SpeG is an SSAT protein found in a variety of bacteria, including the human pathogen Vibrio cholerae. This protein adopts a dodecameric structure and contains an allosteric site, making it unique compared to other SSATs. Currently, we have a limited understanding of the critical structural components of this protein that are required for its allosteric behavior. Therefore, we explored the importance of two key regions of the SpeG protein on its kinetic activity. To achieve this, we created various constructs of the V. cholerae SpeG protein, including point mutations, a deletion, and chimeras with residues from the structurally distinct and non-allosteric human SSAT protein. We measured enzyme kinetic activity toward spermine for ten constructs and crystallized six of them. Ultimately, we identified specific portions of the allosteric loop and the beta6-beta7 structural elements that were critical for enzyme kinetic activity. These results provide a framework for further study of the structure/function relationship of SpeG enzymes from other organisms and clues toward the structural evolution of members of the GNAT family across domains of life.
		+
		+	The Vibrio cholerae SpeG Spermidine/Spermine N-Acetyltransferase Allosteric Loop and beta6-beta7 Structural Elements Are Critical for Kinetic Activity.,Le VTB, Tsimbalyuk S, Lim EQ, Solis A, Gawat D, Boeck P, Lim EQ, Renolo R, Forwood JK, Kuhn ML Front Mol Biosci. 2021 Apr 13;8:645768. doi: 10.3389/fmolb.2021.645768., eCollection 2021. PMID:33928120<ref>PMID:33928120</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kwq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Diamine N-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boeck P]]	+	[[Category: Vibch]]
-	[[Category: Forwood JK]]	+	[[Category: Boeck, P]]
-	[[Category: Gawat D]]	+	[[Category: Forwood, J K]]
-	[[Category: Kuhn ML]]	+	[[Category: Gawat, D]]
-	[[Category: Le ~~VTB~~]]	+	[[Category: Kuhn, M L]]
-	[[Category: Lim EQ]]	+	[[Category: Le, V T.B]]
-	[[Category: Renolo R]]	+	[[Category: Lim, E Q]]
-	[[Category: Solis A]]	+	[[Category: Renolo, R]]
-	[[Category: Tsimbalyuk S]]	+	[[Category: Solis, A]]
		+	[[Category: Tsimbalyuk, S]]
		+	[[Category: Speg enzyme allosteric]]
		+	[[Category: Transferase]]

OCA at 12:37, 16 December 2020

OCA — Wed, 16 Dec 2020 12:37:29 GMT

←Older revision		Revision as of 12:37, 16 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7kwq ~~is ON HOLD~~	+	==Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT==
-		+	<StructureSection load='7kwq' size='340' side='right'caption='[[7kwq]]' scene=''>
-	~~Authors~~: ~~Le, V~~.T.B.~~, Tsimbalyuk, S~~.~~, Lim, E~~.Q.~~, Solis, A~~., ~~Gawat, D~~., ~~Boeck, P~~., ~~Renolo, R~~., ~~Forwood, J~~.K., ~~Kuhn, M~~.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KWQ FirstGlance]. <br>
-	~~Description: Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwq OCA], [http://pdbe.org/7kwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kwq RCSB], [http://www.ebi.ac.uk/pdbsum/7kwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Forwood, J.K~~]]	+	__TOC__
-	[[Category: ~~Gawat, D~~]]	+	</StructureSection>
-	[[Category: ~~Kuhn, M.L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lim, E.Q~~]]	+	[[Category: Boeck P]]
-	[[Category: ~~Tsimbalyuk, S~~]]	+	[[Category: Forwood JK]]
-	[[Category: ~~Boeck, P~~]]	+	[[Category: Gawat D]]
-	[[Category: ~~Le, V.T.B~~]]	+	[[Category: Kuhn ML]]
-	[[Category: Solis, A]]	+	[[Category: Le VTB]]
-	[[Category: ~~Renolo, R~~]]	+	[[Category: Lim EQ]]
		+	[[Category: Renolo R]]
		+	[[Category: Solis A]]
		+	[[Category: Tsimbalyuk S]]

OCA: Protected "7kwq" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2020 05:17:39 GMT

Protected "7kwq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 9 December 2020

OCA: New page: '''Unreleased structure''' The entry 7kwq is ON HOLD Authors: Le, V.T.B., Tsimbalyuk, S., Lim, E.Q., Solis, A., Gawat, D., Boeck, P., Renolo, R., Forwood, J.K., Kuhn, M.L. Description:...

OCA — Wed, 09 Dec 2020 05:17:37 GMT

New page: '''Unreleased structure''' The entry 7kwq is ON HOLD Authors: Le, V.T.B., Tsimbalyuk, S., Lim, E.Q., Solis, A., Gawat, D., Boeck, P., Renolo, R., Forwood, J.K., Kuhn, M.L. Description:...

New page

'''Unreleased structure'''

The entry 7kwq is ON HOLD

Authors: Le, V.T.B., Tsimbalyuk, S., Lim, E.Q., Solis, A., Gawat, D., Boeck, P., Renolo, R., Forwood, J.K., Kuhn, M.L.

Description: Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT
[[Category: Unreleased Structures]]
[[Category: Forwood, J.K]]
[[Category: Gawat, D]]
[[Category: Kuhn, M.L]]
[[Category: Lim, E.Q]]
[[Category: Tsimbalyuk, S]]
[[Category: Boeck, P]]
[[Category: Le, V.T.B]]
[[Category: Solis, A]]
[[Category: Renolo, R]]