7kws - Revision history

OCA at 15:33, 18 October 2023

OCA — Wed, 18 Oct 2023 15:33:04 GMT

←Older revision		Revision as of 15:33, 18 October 2023
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	<StructureSection load='7kws' size='340' side='right'caption='[[7kws]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='7kws' size='340' side='right'caption='[[7kws]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7kws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Camje Camje~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_NCTC_11168_=_ATCC_700819 Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAD:NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE'>NAD</scene>~~, ~~<scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>kfiD, Cj1441c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~192222 CAMJE])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/UDP~~-~~glucose_6~~-~~dehydrogenase UDP~~-~~glucose 6-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kws OCA], [https://pdbe.org/7kws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kws RCSB], [https://www.ebi.ac.uk/pdbsum/7kws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kws ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kws OCA], [https://pdbe.org/7kws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kws RCSB], [https://www.ebi.ac.uk/pdbsum/7kws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kws ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/UDG_CAMJE UDG_CAMJE] Catalyzes the formation of UDP-glucuronic acid which is required for capsular polysaccharide synthesis. Does not catalyze the formation of glucuronamide moiety of the capsular polysaccharide.<ref>PMID:33621065</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camje~~]]	+	[[Category: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-glucose 6-dehydrogenase]]~~	+	[[Category: Raushel FM]]
-	[[Category: Raushel~~, F M~~]]	+	[[Category: Riegert AS]]
-	[[Category: Riegert~~, A S]]~~	+
-	~~[[Category: Carbohydrate]]~~	+
-	~~[[Category: Dehydrogenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Polysaccharide~~]]	+

OCA at 18:07, 10 March 2021

OCA — Wed, 10 Mar 2021 18:07:38 GMT

←Older revision		Revision as of 18:07, 10 March 2021
Line 1:		Line 1:

	==Cj1441 with NAD+ and UDP-glucose==		==Cj1441 with NAD+ and UDP-glucose==
-	<StructureSection load='7kws' size='340' side='right'caption='[[7kws]]' scene=''>	+	<StructureSection load='7kws' size='340' side='right'caption='[[7kws]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7KWS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camje Camje]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7kws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kws OCA], [~~http~~://pdbe.org/7kws PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7kws RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7kws PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7kws ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">kfiD, Cj1441c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192222 CAMJE])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kws OCA], [https://pdbe.org/7kws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kws RCSB], [https://www.ebi.ac.uk/pdbsum/7kws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kws ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Campylobacter jejuni is a pathogenic organism that can cause campylobacteriosis in children and adults. Most commonly, campylobacter infection is brought on by consumption of raw or undercooked poultry, unsanitary drinking water, or pet feces. Surrounding the C. jejuni bacterium is a coat of sugar molecules known as the capsular polysaccharide (CPS). The capsular polysaccharide can be very diverse among the different strains of C. jejuni, and this diversity is considered important for evading the host immune system. Modifications to the CPS of C. jejuni NCTC 11168 include O-methylation, phosphoramidylation, and amidation of glucuronate with either serinol or ethanolamine. The enzymes responsible for amidation of glucuronate are currently unknown. In this study, Cj1441, an enzyme expressed from the CPS biosynthetic gene cluster in C. jejuni NCTC 11168, was shown to catalyze the oxidation of UDP-alpha-d-glucose into UDP-alpha-d-glucuronic acid with NAD(+) as the cofactor. No amide products were found in an attempt to determine whether the putative thioester intermediate formed during the oxidation of UDP-glucose by Cj1441 could be captured in the presence of added amines. The three-dimensional crystal structure of Cj1441 was determined in the presence of NAD(+) and UDP-glucose bound in the active site of the enzyme (Protein Data Bank entry 7KWS). A more thorough bioinformatic analysis of the CPS gene cluster suggests that the amidation activity is localized to the t-terminal half of Cj1438, a bifunctional enzyme that is currently annotated as a sugar transferase.
		+
		+	Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis from Campylobacter jejuni.,Riegert AS, Raushel FM Biochemistry. 2021 Mar 9;60(9):725-734. doi: 10.1021/acs.biochem.0c00953. Epub, 2021 Feb 23. PMID:33621065<ref>PMID:33621065</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kws" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camje]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Raushel FM]]	+	[[Category: UDP-glucose 6-dehydrogenase]]
-	[[Category: Riegert AS]]	+	[[Category: Raushel, F M]]
		+	[[Category: Riegert, A S]]
		+	[[Category: Carbohydrate]]
		+	[[Category: Dehydrogenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Polysaccharide]]

OCA at 05:56, 20 January 2021

OCA — Wed, 20 Jan 2021 05:56:55 GMT

←Older revision		Revision as of 05:56, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7kws ~~is ON HOLD~~	+	==Cj1441 with NAD+ and UDP-glucose==
-		+	<StructureSection load='7kws' size='340' side='right'caption='[[7kws]]' scene=''>
-	~~Authors~~: ~~Riegert~~, A.S., ~~Raushel~~, F.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KWS FirstGlance]. <br>
-	~~Description~~: ~~Cj1441 with NAD+ and UDP~~-~~glucose~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kws OCA], [http://pdbe.org/7kws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kws RCSB], [http://www.ebi.ac.uk/pdbsum/7kws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kws ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Riegert, A.S~~]]	+	__TOC__
-	[[Category: ~~Raushel, F.M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Raushel FM]]
		+	[[Category: Riegert AS]]

OCA: Protected "7kws" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2020 05:17:44 GMT

Protected "7kws" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 9 December 2020

OCA: New page: '''Unreleased structure''' The entry 7kws is ON HOLD Authors: Riegert, A.S., Raushel, F.M. Description: Cj1441 with NAD+ and UDP-glucose Category: Unreleased Structures [[Category:...

OCA — Wed, 09 Dec 2020 05:17:43 GMT

New page: '''Unreleased structure''' The entry 7kws is ON HOLD Authors: Riegert, A.S., Raushel, F.M. Description: Cj1441 with NAD+ and UDP-glucose Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 7kws is ON HOLD

Authors: Riegert, A.S., Raushel, F.M.

Description: Cj1441 with NAD+ and UDP-glucose
[[Category: Unreleased Structures]]
[[Category: Riegert, A.S]]
[[Category: Raushel, F.M]]