7l4s - Revision history

OCA at 09:20, 25 October 2023

OCA — Wed, 25 Oct 2023 09:20:00 GMT

←Older revision		Revision as of 09:20, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7l4s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L4S FirstGlance]. <br>		<table><tr><td colspan='2'>[[7l4s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L4S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l4s OCA], [https://pdbe.org/7l4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l4s RCSB], [https://www.ebi.ac.uk/pdbsum/7l4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l4s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l4s OCA], [https://pdbe.org/7l4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l4s RCSB], [https://www.ebi.ac.uk/pdbsum/7l4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l4s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:29, 7 December 2022

OCA — Wed, 07 Dec 2022 08:29:09 GMT

←Older revision		Revision as of 08:29, 7 December 2022
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	==Crystal structure of the OxyR regulatory domain of Shewanella oneidensis MR-1, reduced form==		==Crystal structure of the OxyR regulatory domain of Shewanella oneidensis MR-1, reduced form==
-	<StructureSection load='7l4s' size='340' side='right'caption='[[7l4s]]' scene=''>	+	<StructureSection load='7l4s' size='340' side='right'caption='[[7l4s]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L4S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7l4s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L4S FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l4s OCA], [https://pdbe.org/7l4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l4s RCSB], [https://www.ebi.ac.uk/pdbsum/7l4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l4s ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l4s OCA], [https://pdbe.org/7l4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l4s RCSB], [https://www.ebi.ac.uk/pdbsum/7l4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l4s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8EHA1_SHEON Q8EHA1_SHEON]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	LysR-type transcriptional regulators (LTTRs), which function in diverse biological processes in prokaryotes, are composed of a conserved structure with an N-terminal DNA-binding domain (DBD) and a C-terminal signal-sensing regulatory domain (RD). LTTRs that sense and respond to the same signal are often functionally exchangeable in bacterial species across wide phyla, but this phenomenon has not been demonstrated for the H2O2-sensing and -responding OxyRs. Here, we systematically examined the biochemical and structural determinants differentiating activator-only OxyRs from dual-activity ones by comparing OxyRs from two Gammaproteobacteria, Escherichia coli and Shewanella oneidensis. Our data show that EcOxyR could function as neither an activator nor a repressor in S. oneidensis. Using SoOxyR-based OxyR chimeras and mutants, we demonstrated that residues 283 to 289, which form the first half of the last C-terminal alpha-helix (alpha10), are critical for the proper function of SoOxyR and cannot be replaced with the EcOxyR counterpart. Crystal structural analysis reveals that alpha10 is important for the oligomerization of SoOxyR, which, unlike EcOxyR, forms several high-order oligomers upon DNA binding. As the mechanisms of OxyR oligomerization vary substantially among bacterial species, our findings underscore the importance of subtle structural features in determining regulatory activities of structurally similar proteins descending from a common ancestor. IMPORTANCE Evolution may drive homologous proteins to be functionally nonexchangeable in different organisms. However, much is unknown about the mechanisms underlying this phenomenon beyond amino acid substitutions. Here, we systematically examined the biochemical and structural determinants differentiating functionally nonexchangeable OxyRs, H2O2-responding transcriptional regulators from two Gammaproteobacteria, Escherichia coli and Shewanella oneidensis. Using SoOxyR-based OxyR chimeras and mutants, we demonstrated that residues 283 to 289, which form the first half of the last C-terminal alpha-helix (alpha10), are critical for the proper function of SoOxyR and cannot be replaced with the EcOxyR counterpart. Crystal structural analysis reveals that this last helix is critical for formation of high-order oligomers upon DNA binding, a phenomenon not observed with EcOxyR. Our findings provide a new dimension to differences in sequence and structural features among bacterial species in determining regulatory activities of homologous regulators.
		+
		+	Functional Irreplaceability of Escherichia coli and Shewanella oneidensis OxyRs Is Critically Determined by Intrinsic Differences in Oligomerization.,Sun W, Fan Y, Wan F, Tao YJ, Gao H mBio. 2022 Jan 25:e0349721. doi: 10.1128/mbio.03497-21. PMID:35073744<ref>PMID:35073744</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7l4s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Shewanella oneidensis MR-1]]
	[[Category: Gao H]]		[[Category: Gao H]]
	[[Category: Tao YJ]]		[[Category: Tao YJ]]

OCA at 07:22, 9 February 2022

OCA — Wed, 09 Feb 2022 07:22:25 GMT

←Older revision		Revision as of 07:22, 9 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7l4s ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the OxyR regulatory domain of Shewanella oneidensis MR-1, reduced form==
-		+	<StructureSection load='7l4s' size='340' side='right'caption='[[7l4s]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7L4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7L4S FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7l4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7l4s OCA], [https://pdbe.org/7l4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7l4s RCSB], [https://www.ebi.ac.uk/pdbsum/7l4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7l4s ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gao H]]
		+	[[Category: Tao YJ]]

OCA at 07:53, 26 May 2021

OCA — Wed, 26 May 2021 07:53:19 GMT

←Older revision		Revision as of 07:53, 26 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7l4s is ON HOLD	+	The entry 7l4s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:14, 28 April 2021

OCA — Wed, 28 Apr 2021 04:14:26 GMT

←Older revision		Revision as of 04:14, 28 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7l4s is ON HOLD ~~until Paper Publication~~	+	The entry 7l4s is ON HOLD

	Authors:		Authors:

OCA at 05:36, 24 February 2021

OCA — Wed, 24 Feb 2021 05:36:17 GMT

←Older revision		Revision as of 05:36, 24 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7l4s is ON HOLD until ~~Feb 15 2022~~	+	The entry 7l4s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 06:44, 27 January 2021

OCA — Wed, 27 Jan 2021 06:44:24 GMT

←Older revision		Revision as of 06:44, 27 January 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7l4s is ON HOLD	+	The entry 7l4s is ON HOLD until Feb 15 2022

	Authors:		Authors:

OCA: Protected "7l4s" [edit=sysop:move=sysop]

OCA — Wed, 30 Dec 2020 05:30:34 GMT

Protected "7l4s" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 30 December 2020

OCA: New page: '''Unreleased structure''' The entry 7l4s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Dec 2020 05:30:33 GMT

New page: '''Unreleased structure''' The entry 7l4s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7l4s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]