7la8 - Revision history

OCA at 15:42, 18 October 2023

OCA — Wed, 18 Oct 2023 15:42:10 GMT

←Older revision		Revision as of 15:42, 18 October 2023
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	==O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc==		==O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc==
-	<StructureSection load='7la8' size='340' side='right'caption='[[7la8]]' scene=''>	+	<StructureSection load='7la8' size='340' side='right'caption='[[7la8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LA8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7la8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Petromyzon_marinus Petromyzon marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LA8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7la8 OCA], [https://pdbe.org/7la8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7la8 RCSB], [https://www.ebi.ac.uk/pdbsum/7la8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7la8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8965Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SGA:O3-SULFONYLGALACTOSE'>SGA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7la8 OCA], [https://pdbe.org/7la8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7la8 RCSB], [https://www.ebi.ac.uk/pdbsum/7la8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7la8 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification. To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression. O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays. 3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase. O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3). The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.
		+
		+	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.,McKitrick TR, Bernard SM, Noll AJ, Collins BC, Goth CK, McQuillan AM, Heimburg-Molinaro J, Herrin BR, Wilson IA, Cooper MD, Cummings RD Commun Biol. 2021 Jun 3;4(1):674. doi: 10.1038/s42003-021-02199-7. PMID:34083726<ref>PMID:34083726</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7la8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Variable lymphocyte receptor 3D structures\|Variable lymphocyte receptor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Petromyzon marinus]]
	[[Category: Bernard SM]]		[[Category: Bernard SM]]
	[[Category: Wilson IA]]		[[Category: Wilson IA]]

OCA at 09:54, 14 July 2021

OCA — Wed, 14 Jul 2021 09:54:19 GMT

←Older revision		Revision as of 09:54, 14 July 2021
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-	'''Unreleased structure'''

-	~~The entry 7la8 is ON HOLD until Paper Publication~~	+	==O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc==
-		+	<StructureSection load='7la8' size='340' side='right'caption='[[7la8]]' scene=''>
-	~~Authors: Bernard, S.M., Wilson, I.A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LA8 FirstGlance]. <br>
-	~~Description:~~ O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7la8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7la8 OCA], [https://pdbe.org/7la8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7la8 RCSB], [https://www.ebi.ac.uk/pdbsum/7la8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7la8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Bernard~~, S.M~~]]	+	__TOC__
-	[[Category: Wilson~~, I.A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bernard SM]]
		+	[[Category: Wilson IA]]

OCA at 07:53, 26 May 2021

OCA — Wed, 26 May 2021 07:53:24 GMT

←Older revision		Revision as of 07:53, 26 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7la8 is ON HOLD	+	The entry 7la8 is ON HOLD until Paper Publication

	Authors: Bernard, S.M., Wilson, I.A.		Authors: Bernard, S.M., Wilson, I.A.

OCA: Protected "7la8" [edit=sysop:move=sysop]

OCA — Wed, 10 Mar 2021 14:39:01 GMT

Protected "7la8" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:39, 10 March 2021

OCA: New page: '''Unreleased structure''' The entry 7la8 is ON HOLD Authors: Bernard, S.M., Wilson, I.A. Description: O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc [[Category...

OCA — Wed, 10 Mar 2021 14:38:59 GMT

New page: '''Unreleased structure''' The entry 7la8 is ON HOLD Authors: Bernard, S.M., Wilson, I.A. Description: O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc [[Category...

New page

'''Unreleased structure'''

The entry 7la8 is ON HOLD

Authors: Bernard, S.M., Wilson, I.A.

Description: O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc
[[Category: Unreleased Structures]]
[[Category: Bernard, S.M]]
[[Category: Wilson, I.A]]