7lhc - Revision history

OCA at 11:14, 14 June 2023

OCA — Wed, 14 Jun 2023 11:14:54 GMT

←Older revision		Revision as of 11:14, 14 June 2023
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	==NMR Solution Structure of [T20K]kalata B1==		==NMR Solution Structure of [T20K]kalata B1==
-	<StructureSection load='7lhc' size='340' side='right'caption='[[7lhc~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7lhc' size='340' side='right'caption='[[7lhc]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7lhc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oldenlandia_affinis Oldenlandia affinis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHC FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhc OCA], [https://pdbe.org/7lhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhc RCSB], [https://www.ebi.ac.uk/pdbsum/7lhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhc ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhc OCA], [https://pdbe.org/7lhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhc RCSB], [https://www.ebi.ac.uk/pdbsum/7lhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KAB1_OLDAF KAB1_OLDAF]] Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.<ref>PMID:17534989</ref> <ref>PMID:12779323</ref>	+	[https://www.uniprot.org/uniprot/KAB1_OLDAF KAB1_OLDAF] Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.<ref>PMID:17534989</ref> <ref>PMID:12779323</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Craik, D J~~]]	+	[[Category: Oldenlandia affinis]]
-	[[Category: ~~Gruber, C W~~]]	+	[[Category: Craik DJ]]
-	[[Category: ~~Harvey, P J~~]]	+	[[Category: Gruber CW]]
-	[[Category: ~~Cck motif]]~~	+	[[Category: Harvey PJ]]
-	~~[[Category: Cyclic peptide]]~~	+
-	~~[[Category: Cyclotide]]~~	+
-	~~[[Category: Knottin]]~~	+
-	~~[[Category: Plant protein~~]]	+

OCA at 06:28, 1 December 2021

OCA — Wed, 01 Dec 2021 06:28:09 GMT

←Older revision		Revision as of 06:28, 1 December 2021
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	==NMR Solution Structure of [T20K]kalata B1==		==NMR Solution Structure of [T20K]kalata B1==
-	<StructureSection load='7lhc' size='340' side='right'caption='[[7lhc]]' scene=''>	+	<StructureSection load='7lhc' size='340' side='right'caption='[[7lhc]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lhc]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHC FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhc OCA], [https://pdbe.org/7lhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhc RCSB], [https://www.ebi.ac.uk/pdbsum/7lhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhc ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhc OCA], [https://pdbe.org/7lhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhc RCSB], [https://www.ebi.ac.uk/pdbsum/7lhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/KAB1_OLDAF KAB1_OLDAF]] Probably participates in a plant defense mechanism. Has antibiotic activity. Has a diuretic effect. Has a uterotonic effect in humans. Active against the Gram-positive S.aureus with a minimum inhibition concentration of approximately 0.2 microM. Relatively ineffective against Gram-negative bacteria such as E.coli and P.aeruginosa. Inhibitory effect on the growth and development of larvae from H.punctigera. The unmodified form has hemolytic activity, the oxidized form lacks hemolytic activity. If the protein is linearized, hemolytic activity is lost.<ref>PMID:17534989</ref> <ref>PMID:12779323</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cyclotide T20K inhibits the proliferation of human immune cells and is currently in clinical trials for multiple sclerosis. Here, we provide novel functional data and mechanistic insights into structure-activity relationships of T20K. Analogs with partial or complete reduction of the cystine knot had loss of function in proliferation experiments. Similarly, an acyclic analog of T20K was inactive in lymphocyte bioassays. The lack of activity of non-native peptide analogs appears to be associated with the ability of cyclotides to interact with and penetrate cell membranes, since cellular uptake studies demonstrated fast fractional transfer only of the native peptide into the cytosol of human immune cells. Therefore, structural differences between cyclic and linear native folded peptides were investigated by NMR to elucidate structure-activity relationships. Acyclic T20K had a less rigid backbone and considerable structural changes in loops 1 and 6 compared to the native cyclic T20K, supporting the idea that the cyclic cystine knot motif is a unique bioactive scaffold. This study provides evidence that this structural motif in cyclotides governs bioactivity, interactions with and transport across biological membranes, and the structural integrity of these peptides. These observations could be useful to understand the structure-activity of other cystine knot proteins due to the structural conservation of the cystine knot motif across evolution and to provide guidance for the design of novel cyclic cysteine-stabilized molecules.
		+
		+	Importance of the Cyclic Cystine Knot Structural Motif for Immunosuppressive Effects of Cyclotides.,Hellinger R, Muratspahic E, Devi S, Koehbach J, Vasileva M, Harvey PJ, Craik DJ, Grundemann C, Gruber CW ACS Chem Biol. 2021 Sep 30. doi: 10.1021/acschembio.1c00524. PMID:34592097<ref>PMID:34592097</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lhc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Craik DJ]]	+	[[Category: Craik, D J]]
-	[[Category: Gruber CW]]	+	[[Category: Gruber, C W]]
-	[[Category: Harvey PJ]]	+	[[Category: Harvey, P J]]
		+	[[Category: Cck motif]]
		+	[[Category: Cyclic peptide]]
		+	[[Category: Cyclotide]]
		+	[[Category: Knottin]]
		+	[[Category: Plant protein]]

OCA at 16:29, 20 October 2021

OCA — Wed, 20 Oct 2021 16:29:31 GMT

←Older revision		Revision as of 16:29, 20 October 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lhc ~~is ON HOLD until Paper Publication~~	+	==NMR Solution Structure of [T20K]kalata B1==
-		+	<StructureSection load='7lhc' size='340' side='right'caption='[[7lhc]]' scene=''>
-	~~Authors~~: ~~Harvey~~, P.J., ~~Craik~~, D.J., ~~Gruber~~, C.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHC FirstGlance]. <br>
-	~~Description: NMR Solution Structure of [T20K]kalata B1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhc OCA], [https://pdbe.org/7lhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhc RCSB], [https://www.ebi.ac.uk/pdbsum/7lhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Gruber, C.W~~]]	+	__TOC__
-	[[Category: ~~Craik, D.J~~]]	+	</StructureSection>
-	[[Category: Harvey~~, P.J~~]]	+	[[Category: Large Structures]]
		+	[[Category: Craik DJ]]
		+	[[Category: Gruber CW]]
		+	[[Category: Harvey PJ]]

OCA at 08:43, 17 February 2021

OCA — Wed, 17 Feb 2021 08:43:58 GMT

←Older revision		Revision as of 08:43, 17 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lhc is ON HOLD	+	The entry 7lhc is ON HOLD until Paper Publication

	Authors: Harvey, P.J., Craik, D.J., Gruber, C.W.		Authors: Harvey, P.J., Craik, D.J., Gruber, C.W.

OCA: Protected "7lhc" [edit=sysop:move=sysop]

OCA — Wed, 27 Jan 2021 06:50:43 GMT

Protected "7lhc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:50, 27 January 2021

OCA: New page: '''Unreleased structure''' The entry 7lhc is ON HOLD Authors: Harvey, P.J., Craik, D.J., Gruber, C.W. Description: NMR Solution Structure of [T20K]kalata B1 [[Category: Unreleased Stru...

OCA — Wed, 27 Jan 2021 06:50:42 GMT

New page: '''Unreleased structure''' The entry 7lhc is ON HOLD Authors: Harvey, P.J., Craik, D.J., Gruber, C.W. Description: NMR Solution Structure of [T20K]kalata B1 [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 7lhc is ON HOLD

Authors: Harvey, P.J., Craik, D.J., Gruber, C.W.

Description: NMR Solution Structure of [T20K]kalata B1
[[Category: Unreleased Structures]]
[[Category: Gruber, C.W]]
[[Category: Craik, D.J]]
[[Category: Harvey, P.J]]