7lhl - Revision history

OCA at 14:59, 6 March 2024

OCA — Wed, 06 Mar 2024 14:59:22 GMT

←Older revision		Revision as of 14:59, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7lhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_smegmatis"_trevisan_1889 "bacillus~~ smegmatis~~" trevisan 1889~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>lhr, ERS451418_01790 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1772~~ "~~Bacillus smegmatis~~" ~~Trevisan 1889])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.6Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'~~>[~~https://en.wikipedia.org/wiki/RNA_helicase RNA helicase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~3.6~~.4.13 3.6.4.13] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Mycobacterium smegmatis Lhr (MsmLhr; 1507-aa) is the founder of a novel clade of bacterial helicases. MsmLhr consists of an N-terminal helicase domain (aa 1-856) with a distinctive tertiary structure (Lhr-Core) and a C-terminal domain (Lhr-CTD) of unknown structure. Here, we report that Escherichia coli Lhr (EcoLhr; 1538-aa) is an ATPase, translocase and ATP-dependent helicase. Like MsmLhr, EcoLhr translocates 3' to 5' on ssDNA and unwinds secondary structures en route, with RNA:DNA hybrid being preferred versus DNA:DNA duplex. The ATPase and translocase activities of EcoLhr inhere to its 877-aa Core domain. Full-length EcoLhr and MsmLhr have homo-oligomeric quaternary structures in solution, whereas their respective Core domains are monomers. The MsmLhr CTD per se is a homo-oligomer in solution. We employed cryo-EM to solve the structure of the CTD of full-length MsmLhr. The CTD protomer is composed of a series of five winged-helix (WH) modules and a beta-barrel module. The CTD adopts a unique homo-tetrameric quaternary structure. A Lhr-CTD subdomain, comprising three tandem WH modules and the beta-barrel, is structurally homologous to AlkZ, a bacterial DNA glycosylase that recognizes and excises inter-strand DNA crosslinks. This homology is noteworthy given that Lhr is induced in mycobacteria exposed to the inter-strand crosslinker mitomycin C.

-	Oligomeric quaternary structure of Escherichia coli and Mycobacterium smegmatis Lhr helicases is nucleated by a novel C-terminal domain composed of five winged-helix modules.,Warren GM, Wang J, Patel DJ, Shuman S Nucleic Acids Res. 2021 Mar 21. pii: 6179347. doi: 10.1093/nar/gkab145. PMID:33744958<ref>PMID:33744958</ref>	+	==See Also==
-		+	*[[Helicase 3D structures\|Helicase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7lhl" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus smegmatis trevisan 1889]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RNA helicase~~]]	+	[[Category: Mycolicibacterium smegmatis]]
-	[[Category: Patel~~, D J~~]]	+	[[Category: Patel DJ]]
-	[[Category: Shuman, S]]	+	[[Category: Shuman S]]
-	[[Category: Wang, J]]	+	[[Category: Wang J]]
-	[[Category: Warren~~, G M]]~~	+	[[Category: Warren GM]]
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Winged helix~~]]	+

OCA at 09:14, 5 May 2021

OCA — Wed, 05 May 2021 09:14:34 GMT

←Older revision		Revision as of 09:14, 5 May 2021
Line 1:		Line 1:

	==cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain==		==cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain==
-	<StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]]' scene=''>	+	<StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lhr, ERS451418_01790 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 "Bacillus smegmatis" Trevisan 1889])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium smegmatis Lhr (MsmLhr; 1507-aa) is the founder of a novel clade of bacterial helicases. MsmLhr consists of an N-terminal helicase domain (aa 1-856) with a distinctive tertiary structure (Lhr-Core) and a C-terminal domain (Lhr-CTD) of unknown structure. Here, we report that Escherichia coli Lhr (EcoLhr; 1538-aa) is an ATPase, translocase and ATP-dependent helicase. Like MsmLhr, EcoLhr translocates 3' to 5' on ssDNA and unwinds secondary structures en route, with RNA:DNA hybrid being preferred versus DNA:DNA duplex. The ATPase and translocase activities of EcoLhr inhere to its 877-aa Core domain. Full-length EcoLhr and MsmLhr have homo-oligomeric quaternary structures in solution, whereas their respective Core domains are monomers. The MsmLhr CTD per se is a homo-oligomer in solution. We employed cryo-EM to solve the structure of the CTD of full-length MsmLhr. The CTD protomer is composed of a series of five winged-helix (WH) modules and a beta-barrel module. The CTD adopts a unique homo-tetrameric quaternary structure. A Lhr-CTD subdomain, comprising three tandem WH modules and the beta-barrel, is structurally homologous to AlkZ, a bacterial DNA glycosylase that recognizes and excises inter-strand DNA crosslinks. This homology is noteworthy given that Lhr is induced in mycobacteria exposed to the inter-strand crosslinker mitomycin C.
		+
		+	Oligomeric quaternary structure of Escherichia coli and Mycobacterium smegmatis Lhr helicases is nucleated by a novel C-terminal domain composed of five winged-helix modules.,Warren GM, Wang J, Patel DJ, Shuman S Nucleic Acids Res. 2021 Mar 21. pii: 6179347. doi: 10.1093/nar/gkab145. PMID:33744958<ref>PMID:33744958</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lhl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus smegmatis trevisan 1889]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Patel DJ]]	+	[[Category: RNA helicase]]
-	[[Category: Shuman S]]	+	[[Category: Patel, D J]]
-	[[Category: Wang J]]	+	[[Category: Shuman, S]]
-	[[Category: Warren GM]]	+	[[Category: Wang, J]]
		+	[[Category: Warren, G M]]
		+	[[Category: Helicase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Winged helix]]

OCA at 06:52, 7 April 2021

OCA — Wed, 07 Apr 2021 06:52:59 GMT

←Older revision		Revision as of 06:52, 7 April 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7lhl is ~~ON HOLD until Paper Publication~~	+	==cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain==
-		+	<StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Patel DJ]]
		+	[[Category: Shuman S]]
		+	[[Category: Wang J]]
		+	[[Category: Warren GM]]

OCA at 09:16, 10 February 2021

OCA — Wed, 10 Feb 2021 09:16:35 GMT

←Older revision		Revision as of 09:16, 10 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lhl is ON HOLD	+	The entry 7lhl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7lhl" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2021 09:29:14 GMT

Protected "7lhl" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:29, 3 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lhl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Feb 2021 09:29:13 GMT

New page: '''Unreleased structure''' The entry 7lhl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7lhl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]