7lif - Revision history

OCA at 20:20, 16 November 2022

OCA — Wed, 16 Nov 2022 20:20:56 GMT

←Older revision		Revision as of 20:20, 16 November 2022
Line 7:		Line 7:
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q47NX9_THEFY Q47NX9_THEFY]]	+	[https://www.uniprot.org/uniprot/Q47NX9_THEFY Q47NX9_THEFY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:44, 7 September 2022

OCA — Wed, 07 Sep 2022 19:44:29 GMT

←Older revision		Revision as of 19:44, 7 September 2022
Line 1:		Line 1:

	==Aspartimidylated omega ester peptide fuscimiditide==		==Aspartimidylated omega ester peptide fuscimiditide==
-	<StructureSection load='7lif' size='340' side='right'caption='[[7lif~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='7lif' size='340' side='right'caption='[[7lif]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7lif]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lif OCA], [https://pdbe.org/7lif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lif RCSB], [https://www.ebi.ac.uk/pdbsum/7lif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lif ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lif OCA], [https://pdbe.org/7lif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lif RCSB], [https://www.ebi.ac.uk/pdbsum/7lif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lif ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/Q47NX9_THEFY Q47NX9_THEFY]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Elashal, H E~~]]	+	[[Category: Thermobifida fusca YX]]
-	[[Category: ~~Link, A J~~]]	+	[[Category: Elashal HE]]
-	[[Category: ~~Aspartimidylated omega ester peptide]]~~	+	[[Category: Link AJ]]
-	~~[[Category: Omega ester peptide]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 06:42, 31 August 2022

OCA — Wed, 31 Aug 2022 06:42:15 GMT

←Older revision		Revision as of 06:42, 31 August 2022
Line 1:		Line 1:

	==Aspartimidylated omega ester peptide fuscimiditide==		==Aspartimidylated omega ester peptide fuscimiditide==
-	<StructureSection load='7lif' size='340' side='right'caption='[[7lif]]' scene=''>	+	<StructureSection load='7lif' size='340' side='right'caption='[[7lif]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lif]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lif OCA], [https://pdbe.org/7lif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lif RCSB], [https://www.ebi.ac.uk/pdbsum/7lif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lif ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lif OCA], [https://pdbe.org/7lif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lif RCSB], [https://www.ebi.ac.uk/pdbsum/7lif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lif ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microviridins and other omega-ester-linked peptides, collectively known as graspetides, are characterized by side-chain-side-chain linkages installed by ATP-grasp enzymes. Here we report the discovery of a family of graspetides, the gene clusters of which also encode an O-methyltransferase with homology to the protein repair catalyst protein L-isoaspartyl methyltransferase. Using heterologous expression, we produced fuscimiditide, a ribosomally synthesized and post-translationally modified peptide (RiPP). NMR analysis of fuscimiditide revealed that the peptide contains two ester cross-links forming a stem-loop macrocycle. Furthermore, an unusually stable aspartimide moiety is found within the loop macrocycle. We fully reconstituted fuscimiditide biosynthesis in vitro including formation of the ester and aspartimide moieties. The aspartimide moiety embedded in fuscimiditide hydrolyses regioselectively to isoaspartate. Surprisingly, this isoaspartate-containing peptide is also a substrate for the L-isoaspartyl methyltransferase homologue, thus driving any hydrolysis products back to the aspartimide form. Whereas an aspartimide is often considered a nuisance product in protein formulations, our data suggest that some RiPPs have aspartimide residues intentionally installed via enzymatic activity.
		+
		+	Biosynthesis and characterization of fuscimiditide, an aspartimidylated graspetide.,Elashal HE, Koos JD, Cheung-Lee WL, Choi B, Cao L, Richardson MA, White HL, Link AJ Nat Chem. 2022 Aug 18. pii: 10.1038/s41557-022-01022-y. doi:, 10.1038/s41557-022-01022-y. PMID:35982233<ref>PMID:35982233</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lif" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Elashal HE]]	+	[[Category: Elashal, H E]]
-	[[Category: Link AJ]]	+	[[Category: Link, A J]]
		+	[[Category: Aspartimidylated omega ester peptide]]
		+	[[Category: Omega ester peptide]]
		+	[[Category: Unknown function]]

OCA at 07:22, 9 February 2022

OCA — Wed, 09 Feb 2022 07:22:43 GMT

←Older revision		Revision as of 07:22, 9 February 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7lif ~~is ON HOLD until Paper Publication~~	+	==Aspartimidylated omega ester peptide fuscimiditide==
-		+	<StructureSection load='7lif' size='340' side='right'caption='[[7lif]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIF FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lif OCA], [https://pdbe.org/7lif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lif RCSB], [https://www.ebi.ac.uk/pdbsum/7lif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lif ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Elashal HE]]
		+	[[Category: Link AJ]]

OCA at 08:44, 17 February 2021

OCA — Wed, 17 Feb 2021 08:44:12 GMT

←Older revision		Revision as of 08:44, 17 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lif is ON HOLD	+	The entry 7lif is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7lif" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2021 09:31:18 GMT

Protected "7lif" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:31, 3 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lif is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Feb 2021 09:31:16 GMT

New page: '''Unreleased structure''' The entry 7lif is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7lif is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]