7lik - Revision history

OCA at 19:38, 29 May 2024

OCA — Wed, 29 May 2024 19:38:09 GMT

←Older revision		Revision as of 19:38, 29 May 2024
Line 3:		Line 3:
	<StructureSection load='7lik' size='340' side='right'caption='[[7lik]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='7lik' size='340' side='right'caption='[[7lik]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7lik]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIK FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.91Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lik OCA], [https://pdbe.org/7lik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lik RCSB], [https://www.ebi.ac.uk/pdbsum/7lik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lik ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lik OCA], [https://pdbe.org/7lik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lik RCSB], [https://www.ebi.ac.uk/pdbsum/7lik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lik ProSAT]</span></td></tr>
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/MARIT_THEMA MARIT_THEMA]] Protease that exhibits activity toward chymotrypsin and trypsin substrates. May have antibacterial activity.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Protein cages are a common architectural motif used by living organisms to compartmentalize and control biochemical reactions. While engineered protein cages have featured in the construction of nanoreactors and synthetic organelles, relatively little is known about the underlying molecular parameters that govern stability and flux through their pores. In this work, we systematically designed 24 variants of the Thermotoga maritima encapsulin cage, featuring pores of different sizes and charges. Twelve pore variants were successfully assembled and purified, including eight designs with exceptional thermal stability. While negatively charged mutations were better tolerated, we were able to form stable assemblies covering a full range of pore sizes and charges, as observed in seven new cryo-EM structures at 2.5- to 3.6-A resolution. Molecular dynamics simulations and stopped-flow experiments revealed the importance of considering both pore size and charge, together with flexibility and rate-determining steps, when designing protein cages for controlling molecular flux.
-
-	Pore structure controls stability and molecular flux in engineered protein cages.,Adamson LSR, Tasneem N, Andreas MP, Close W, Jenner EN, Szyszka TN, Young R, Cheah LC, Norman A, MacDermott-Opeskin HI, O'Mara ML, Sainsbury F, Giessen TW, Lau YH Sci Adv. 2022 Feb 4;8(5):eabl7346. doi: 10.1126/sciadv.abl7346. Epub 2022 Feb 4. PMID:35119930<ref>PMID:35119930</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7lik" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adamson, L]]	+	[[Category: Adamson L]]
-	[[Category: Andreas~~, M P~~]]	+	[[Category: Andreas MP]]
-	[[Category: Close, W]]	+	[[Category: Close W]]
-	[[Category: Giessen, T]]	+	[[Category: Giessen T]]
-	[[Category: Lau~~, Y H~~]]	+	[[Category: Lau YH]]
-	[[Category: Tasneem, N]]	+	[[Category: Tasneem N]]
-	~~[[Category: Encapsulin]]~~	+
-	~~[[Category: Nanocompartment]]~~	+
-	~~[[Category: Virus like particle~~]]	+

OCA at 11:02, 16 February 2022

OCA — Wed, 16 Feb 2022 11:02:35 GMT

←Older revision		Revision as of 11:02, 16 February 2022
Line 1:		Line 1:

	==Thermotoga maritima Encapsulin Nanocompartment Pore Mutant S1R==		==Thermotoga maritima Encapsulin Nanocompartment Pore Mutant S1R==
-	<StructureSection load='7lik' size='340' side='right'caption='[[7lik]]' scene=''>	+	<StructureSection load='7lik' size='340' side='right'caption='[[7lik]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lik]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lik OCA], [https://pdbe.org/7lik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lik RCSB], [https://www.ebi.ac.uk/pdbsum/7lik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lik ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lik OCA], [https://pdbe.org/7lik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lik RCSB], [https://www.ebi.ac.uk/pdbsum/7lik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lik ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/MARIT_THEMA MARIT_THEMA]] Protease that exhibits activity toward chymotrypsin and trypsin substrates. May have antibacterial activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein cages are a common architectural motif used by living organisms to compartmentalize and control biochemical reactions. While engineered protein cages have featured in the construction of nanoreactors and synthetic organelles, relatively little is known about the underlying molecular parameters that govern stability and flux through their pores. In this work, we systematically designed 24 variants of the Thermotoga maritima encapsulin cage, featuring pores of different sizes and charges. Twelve pore variants were successfully assembled and purified, including eight designs with exceptional thermal stability. While negatively charged mutations were better tolerated, we were able to form stable assemblies covering a full range of pore sizes and charges, as observed in seven new cryo-EM structures at 2.5- to 3.6-A resolution. Molecular dynamics simulations and stopped-flow experiments revealed the importance of considering both pore size and charge, together with flexibility and rate-determining steps, when designing protein cages for controlling molecular flux.
		+
		+	Pore structure controls stability and molecular flux in engineered protein cages.,Adamson LSR, Tasneem N, Andreas MP, Close W, Jenner EN, Szyszka TN, Young R, Cheah LC, Norman A, MacDermott-Opeskin HI, O'Mara ML, Sainsbury F, Giessen TW, Lau YH Sci Adv. 2022 Feb 4;8(5):eabl7346. doi: 10.1126/sciadv.abl7346. Epub 2022 Feb 4. PMID:35119930<ref>PMID:35119930</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lik" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adamson L]]	+	[[Category: Adamson, L]]
-	[[Category: Andreas MP]]	+	[[Category: Andreas, M P]]
-	[[Category: Close W]]	+	[[Category: Close, W]]
-	[[Category: Giessen T]]	+	[[Category: Giessen, T]]
-	[[Category: Lau YH]]	+	[[Category: Lau, Y H]]
-	[[Category: Tasneem N]]	+	[[Category: Tasneem, N]]
		+	[[Category: Encapsulin]]
		+	[[Category: Nanocompartment]]
		+	[[Category: Virus like particle]]

OCA at 07:22, 9 February 2022

OCA — Wed, 09 Feb 2022 07:22:49 GMT

←Older revision		Revision as of 07:22, 9 February 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7lik is ~~ON HOLD until Paper Publication~~	+	==Thermotoga maritima Encapsulin Nanocompartment Pore Mutant S1R==
-		+	<StructureSection load='7lik' size='340' side='right'caption='[[7lik]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LIK FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lik OCA], [https://pdbe.org/7lik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lik RCSB], [https://www.ebi.ac.uk/pdbsum/7lik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lik ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Adamson L]]
		+	[[Category: Andreas MP]]
		+	[[Category: Close W]]
		+	[[Category: Giessen T]]
		+	[[Category: Lau YH]]
		+	[[Category: Tasneem N]]

OCA at 05:37, 24 February 2021

OCA — Wed, 24 Feb 2021 05:37:59 GMT

←Older revision		Revision as of 05:37, 24 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lik is ON HOLD	+	The entry 7lik is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7lik" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2021 09:31:34 GMT

Protected "7lik" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:31, 3 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lik is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Feb 2021 09:31:33 GMT

New page: '''Unreleased structure''' The entry 7lik is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7lik is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]