OCA at 15:52, 18 October 2023

OCA — Wed, 18 Oct 2023 15:52:44 GMT

←Older revision		Revision as of 15:52, 18 October 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m43.151==
-	<StructureSection load='7lkg' size='340' side='right'caption='[[7lkg]]' scene=''>	+	<StructureSection load='7lkg' size='340' side='right'caption='[[7lkg]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lkg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LKG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lkg OCA], [https://pdbe.org/7lkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lkg RCSB], [https://www.ebi.ac.uk/pdbsum/7lkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lkg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lkg OCA], [https://pdbe.org/7lkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lkg RCSB], [https://www.ebi.ac.uk/pdbsum/7lkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lkg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CSP_PLAF7 CSP_PLAF7] Essential sporozoite protein (PubMed:29554084, PubMed:32150583). In the mosquito vector, required for sporozoite development in the oocyst, migration through the vector hemolymph and entry into the vector salivary glands (By similarity). In the vertebrate host, required for sporozoite migration through the host dermis and infection of host hepatocytes (PubMed:29554084, PubMed:32150583). Binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes (By similarity).[UniProtKB:P23093]<ref>PMID:29554084</ref> <ref>PMID:32150583</ref> In the vertebrate host, binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes and is required for sporozoite invasion of the host hepatocytes.[UniProtKB:P23093]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Repeat antigens, such as the Plasmodium falciparum circumsporozoite protein (PfCSP), use both sequence degeneracy and structural diversity to evade the immune response. A few PfCSP-directed antibodies have been identified that are effective at preventing malaria infection, including CIS43, but how these repeat-targeting antibodies might be improved has been unclear. Here, we engineered a humanized mouse model in which B cells expressed inferred human germline CIS43 (iGL-CIS43) B cell receptors and used both vaccination and bioinformatic analysis to obtain variant CIS43 antibodies with improved protective capacity. One such antibody, iGL-CIS43.D3, was significantly more potent than the current best-in-class PfCSP-directed antibody. We found that vaccination with a junctional epitope peptide was more effective than full-length PfCSP at recruiting iGL-CIS43 B cells to germinal centers. Structure-function analysis revealed multiple somatic hypermutations that combinatorically improved protection. This mouse model can thus be used to understand vaccine immunogens and to develop highly potent anti-malarial antibodies.
		+
		+	Vaccination in a humanized mouse model elicits highly protective PfCSP-targeting anti-malarial antibodies.,Kratochvil S, Shen CH, Lin YC, Xu K, Nair U, Da Silva Pereira L, Tripathi P, Arnold J, Chuang GY, Melzi E, Schon A, Zhang B, Dillon M, Bonilla B, Flynn BJ, Kirsch KH, Kisalu NK, Kiyuka PK, Liu T, Ou L, Pancera M, Rawi R, Reveiz M, Seignon K, Wang LT, Waring MT, Warner J, Yang Y, Francica JR, Idris AH, Seder RA, Kwong PD, Batista FD Immunity. 2021 Dec 14;54(12):2859-2876.e7. doi: 10.1016/j.immuni.2021.10.017. , Epub 2021 Nov 16. PMID:34788599<ref>PMID:34788599</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lkg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Plasmodium falciparum 3D7]]
		+	[[Category: Kwong PD]]
		+	[[Category: Xu K]]

OCA at 16:47, 15 December 2021

OCA — Wed, 15 Dec 2021 16:47:51 GMT

←Older revision		Revision as of 16:47, 15 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7lkg ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7lkg' size='340' side='right'caption='[[7lkg]]' scene=''>
-	~~Authors~~: ~~Xu, K~~.~~, Kwong, P~~.D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of PfCSP peptide 21 with vaccine~~-~~elicited human anti-malaria antibody m43~~.~~151~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lkg OCA], [https://pdbe.org/7lkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lkg RCSB], [https://www.ebi.ac.uk/pdbsum/7lkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lkg ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Kwong, P.D~~]]	+	__TOC__
-	[[Category: ~~Xu, K~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:27, 24 March 2021

OCA — Wed, 24 Mar 2021 05:27:54 GMT

←Older revision		Revision as of 05:27, 24 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lkg is ON HOLD	+	The entry 7lkg is ON HOLD until Paper Publication

	Authors: Xu, K., Kwong, P.D.		Authors: Xu, K., Kwong, P.D.

OCA: Protected "7lkg" [edit=sysop:move=sysop]

OCA — Wed, 10 Feb 2021 09:19:27 GMT

Protected "7lkg" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 10 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lkg is ON HOLD Authors: Xu, K., Kwong, P.D. Description: Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m...

OCA — Wed, 10 Feb 2021 09:19:26 GMT

New page: '''Unreleased structure''' The entry 7lkg is ON HOLD Authors: Xu, K., Kwong, P.D. Description: Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m...

New page

'''Unreleased structure'''

The entry 7lkg is ON HOLD

Authors: Xu, K., Kwong, P.D.

Description: Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m43.151
[[Category: Unreleased Structures]]
[[Category: Kwong, P.D]]
[[Category: Xu, K]]

7lkg - Revision history

OCA at 15:52, 18 October 2023

OCA at 16:47, 15 December 2021

OCA at 05:27, 24 March 2021

OCA: Protected "7lkg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7lkg is ON HOLD Authors: Xu, K., Kwong, P.D. Description: Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m...