7lnn - Revision history

OCA at 15:54, 18 October 2023

OCA — Wed, 18 Oct 2023 15:54:23 GMT

←Older revision		Revision as of 15:54, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7lnn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNN FirstGlance]. <br>		<table><tr><td colspan='2'>[[7lnn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PPK:(DIPHOSPHONO)AMINOPHOSPHONIC+ACID'>PPK</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metK, HMPREF1611_00479 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1268998 Escherichia coli 908573])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PPK:(DIPHOSPHONO)AMINOPHOSPHONIC+ACID'>PPK</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnn OCA], [https://pdbe.org/7lnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnn RCSB], [https://www.ebi.ac.uk/pdbsum/7lnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnn OCA], [https://pdbe.org/7lnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnn RCSB], [https://www.ebi.ac.uk/pdbsum/7lnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]	+	[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7lnn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7lnn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[S-adenosylmethionine synthetase 3D structures\|S-adenosylmethionine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Escherichia coli 908573]]		[[Category: Escherichia coli 908573]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Methionine adenosyltransferase]]~~	+	[[Category: Jackson CJ]]
-	[[Category: Jackson~~, C J~~]]	+	[[Category: Tan LL]]
-	[[Category: Tan~~, L L]]~~	+
-	~~[[Category: S-adenosyl methionine transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:27, 2 March 2022

OCA — Wed, 02 Mar 2022 07:27:58 GMT

←Older revision		Revision as of 07:27, 2 March 2022
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	==E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate==		==E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate==
-	<StructureSection load='7lnn' size='340' side='right'caption='[[7lnn]]' scene=''>	+	<StructureSection load='7lnn' size='340' side='right'caption='[[7lnn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lnn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_908573 Escherichia coli 908573]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnn OCA], [https://pdbe.org/7lnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnn RCSB], [https://www.ebi.ac.uk/pdbsum/7lnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnn ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PPK:(DIPHOSPHONO)AMINOPHOSPHONIC+ACID'>PPK</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">metK, HMPREF1611_00479 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1268998 Escherichia coli 908573])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnn OCA], [https://pdbe.org/7lnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnn RCSB], [https://www.ebi.ac.uk/pdbsum/7lnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/V0ZE41_ECOLX V0ZE41_ECOLX]] Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.[HAMAP-Rule:MF_00086]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein conformational changes can facilitate the binding of noncognate substrates and underlying promiscuous activities. However, the contribution of substrate conformational dynamics to this process is comparatively poorly understood. Here, we analyze human (hMAT2A) and Escherichia coli (eMAT) methionine adenosyltransferases that have identical active sites but different substrate specificity. In the promiscuous hMAT2A, noncognate substrates bind in a stable conformation to allow catalysis. In contrast, noncognate substrates sample stable productive binding modes less frequently in eMAT owing to altered mobility in the enzyme active site. Different cellular concentrations of substrates likely drove the evolutionary divergence of substrate specificity in these orthologues. The observation of catalytic promiscuity in hMAT2A led to the detection of a new human metabolite, methyl thioguanosine, that is produced at elevated levels in a cancer cell line. This work establishes that identical active sites can result in different substrate specificity owing to the effects of substrate and enzyme dynamics.
		+
		+	Substrate Dynamics Contribute to Enzymatic Specificity in Human and Bacterial Methionine Adenosyltransferases.,Gade M, Tan LL, Damry AM, Sandhu M, Brock JS, Delaney A, Villar-Briones A, Jackson CJ, Laurino P JACS Au. 2021 Nov 19;1(12):2349-2360. doi: 10.1021/jacsau.1c00464. eCollection, 2021 Dec 27. PMID:34977903<ref>PMID:34977903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lnn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli 908573]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jackson CJ]]	+	[[Category: Methionine adenosyltransferase]]
-	[[Category: Tan LL]]	+	[[Category: Jackson, C J]]
		+	[[Category: Tan, L L]]
		+	[[Category: S-adenosyl methionine transferase]]
		+	[[Category: Transferase]]

OCA at 10:34, 31 March 2021

OCA — Wed, 31 Mar 2021 10:34:03 GMT

←Older revision		Revision as of 10:34, 31 March 2021
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-	'''Unreleased structure'''

-	~~The entry 7lnn is ON HOLD~~	+	==E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate==
-		+	<StructureSection load='7lnn' size='340' side='right'caption='[[7lnn]]' scene=''>
-	~~Authors: Tan, L.L., Jackson, C.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNN FirstGlance]. <br>
-	~~Description:~~ E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnn OCA], [https://pdbe.org/7lnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnn RCSB], [https://www.ebi.ac.uk/pdbsum/7lnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnn ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Jackson~~, C.J~~]]	+	__TOC__
-	[[Category: Tan~~, L.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jackson CJ]]
		+	[[Category: Tan LL]]

OCA: Protected "7lnn" [edit=sysop:move=sysop]

OCA — Wed, 17 Feb 2021 08:47:32 GMT

Protected "7lnn" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:47, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7lnn is ON HOLD Authors: Tan, L.L., Jackson, C.J. Description: E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotri...

OCA — Wed, 17 Feb 2021 08:47:31 GMT

New page: '''Unreleased structure''' The entry 7lnn is ON HOLD Authors: Tan, L.L., Jackson, C.J. Description: E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotri...

New page

'''Unreleased structure'''

The entry 7lnn is ON HOLD

Authors: Tan, L.L., Jackson, C.J.

Description: E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate
[[Category: Unreleased Structures]]
[[Category: Jackson, C.J]]
[[Category: Tan, L.L]]