7lur - Revision history

OCA at 16:00, 18 October 2023

OCA — Wed, 18 Oct 2023 16:00:07 GMT

←Older revision		Revision as of 16:00, 18 October 2023
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-	====	+	==Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized Version of the B-domain (Mini-Z) from Protein A Called Z34C==
-	<StructureSection load='7lur' size='340' side='right'caption='[[7lur]]' scene=''>	+	<StructureSection load='7lur' size='340' side='right'caption='[[7lur]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lur]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lur OCA], [https://pdbe.org/7lur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lur RCSB], [https://www.ebi.ac.uk/pdbsum/7lur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lur ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lur OCA], [https://pdbe.org/7lur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lur RCSB], [https://www.ebi.ac.uk/pdbsum/7lur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lur ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/IGHG1_HUMAN IGHG1_HUMAN] Defects in IGHG1 are a cause of multiple myeloma (MM) [MIM:[https://omim.org/entry/254500 254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IGHG1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGHG1_HUMAN IGHG1_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bispecific antibodies (Bispecifics) demonstrate exceptional clinical potential to address some of the most complex diseases. However, Bispecific production in a single cell often requires the correct pairing of multiple polypeptide chains for desired assembly. This is a considerable hurdle that hinders the development of many immunoglobulin G (IgG)-like bispecific formats. Our approach focuses on the rational engineering of charged residues to facilitate the chain pairing of distinct heavy chains (HC). Here, we deploy structure-guided protein design to engineer charge pair mutations (CPMs) placed in the CH3-CH3' interface of the fragment crystallizable (Fc) region of an antibody (Ab) to correctly steer heavy chain pairing. When used in combination with our stable effector functionless 2 (SEFL2.2) technology, we observed high pairing efficiency without significant losses in expression yields. Furthermore, we investigate the relationship between CPMs and the sequence diversity in the parental antibodies, proposing a rational strategy to deploy these engineering technologies.
		+
		+	Next generation Fc scaffold for multispecific antibodies.,Estes B, Sudom A, Gong D, Whittington DA, Li V, Mohr C, Li D, Riley TP, Shi SD, Zhang J, Garces F, Wang Z iScience. 2021 Nov 15;24(12):103447. doi: 10.1016/j.isci.2021.103447. eCollection , 2021 Dec 17. PMID:34877503<ref>PMID:34877503</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lur" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Staphylococcus aureus]]
		+	[[Category: Garces F]]
		+	[[Category: Sudom A]]
		+	[[Category: Wang Z]]

OCA at 09:26, 15 September 2021

OCA — Wed, 15 Sep 2021 09:26:44 GMT

←Older revision		Revision as of 09:26, 15 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lur ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7lur' size='340' side='right'caption='[[7lur]]' scene=''>
-	~~Authors~~: ~~Sudom, A~~.~~, Garces, F~~.~~, Wang, Z~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to~~ a ~~Minimized Version of~~ the ~~B-domain (Mini-Z) from Protein A Called Z34C~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lur OCA], [https://pdbe.org/7lur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lur RCSB], [https://www.ebi.ac.uk/pdbsum/7lur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lur ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Garces~~, F]]	+	__TOC__
-	[[Category: ~~Sudom, A~~]]	+	</StructureSection>
-	[[Category: ~~Wang,~~ Z]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:20, 31 March 2021

OCA — Wed, 31 Mar 2021 09:20:45 GMT

←Older revision		Revision as of 09:20, 31 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lur is ON HOLD	+	The entry 7lur is ON HOLD until Paper Publication

	Authors: Sudom, A., Garces, F., Wang, Z.		Authors: Sudom, A., Garces, F., Wang, Z.
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	Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized Version of the B-domain (Mini-Z) from Protein A Called Z34C		Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized Version of the B-domain (Mini-Z) from Protein A Called Z34C
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wang, Z]]
	[[Category: Garces, F]]		[[Category: Garces, F]]
	[[Category: Sudom, A]]		[[Category: Sudom, A]]
		+	[[Category: Wang, Z]]

OCA: Protected "7lur" [edit=sysop:move=sysop]

OCA — Wed, 03 Mar 2021 13:27:35 GMT

Protected "7lur" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:27, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7lur is ON HOLD Authors: Sudom, A., Garces, F., Wang, Z. Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized ...

OCA — Wed, 03 Mar 2021 13:27:34 GMT

New page: '''Unreleased structure''' The entry 7lur is ON HOLD Authors: Sudom, A., Garces, F., Wang, Z. Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized ...

New page

'''Unreleased structure'''

The entry 7lur is ON HOLD

Authors: Sudom, A., Garces, F., Wang, Z.

Description: Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized Version of the B-domain (Mini-Z) from Protein A Called Z34C
[[Category: Unreleased Structures]]
[[Category: Wang, Z]]
[[Category: Garces, F]]
[[Category: Sudom, A]]