7lus - Revision history

OCA at 16:00, 18 October 2023

OCA — Wed, 18 Oct 2023 16:00:08 GMT

←Older revision		Revision as of 16:00, 18 October 2023
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-	====	+	==IgG2 Fc Charge Pair Mutation version 1 (CPMv1)==
-	<StructureSection load='7lus' size='340' side='right'caption='[[7lus]]' scene=''>	+	<StructureSection load='7lus' size='340' side='right'caption='[[7lus]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lus]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LUS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lus OCA], [https://pdbe.org/7lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lus RCSB], [https://www.ebi.ac.uk/pdbsum/7lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lus ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lus OCA], [https://pdbe.org/7lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lus RCSB], [https://www.ebi.ac.uk/pdbsum/7lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lus ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/IGHG2_HUMAN IGHG2_HUMAN] Recurrent infections associated with rare immunoglobulin isotypes deficiency.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGHG2_HUMAN IGHG2_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bispecific antibodies (Bispecifics) demonstrate exceptional clinical potential to address some of the most complex diseases. However, Bispecific production in a single cell often requires the correct pairing of multiple polypeptide chains for desired assembly. This is a considerable hurdle that hinders the development of many immunoglobulin G (IgG)-like bispecific formats. Our approach focuses on the rational engineering of charged residues to facilitate the chain pairing of distinct heavy chains (HC). Here, we deploy structure-guided protein design to engineer charge pair mutations (CPMs) placed in the CH3-CH3' interface of the fragment crystallizable (Fc) region of an antibody (Ab) to correctly steer heavy chain pairing. When used in combination with our stable effector functionless 2 (SEFL2.2) technology, we observed high pairing efficiency without significant losses in expression yields. Furthermore, we investigate the relationship between CPMs and the sequence diversity in the parental antibodies, proposing a rational strategy to deploy these engineering technologies.
		+
		+	Next generation Fc scaffold for multispecific antibodies.,Estes B, Sudom A, Gong D, Whittington DA, Li V, Mohr C, Li D, Riley TP, Shi SD, Zhang J, Garces F, Wang Z iScience. 2021 Nov 15;24(12):103447. doi: 10.1016/j.isci.2021.103447. eCollection , 2021 Dec 17. PMID:34877503<ref>PMID:34877503</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lus" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Garces F]]
		+	[[Category: Sudom A]]
		+	[[Category: Wang Z]]
		+	[[Category: Whittington D]]

OCA at 09:26, 15 September 2021

OCA — Wed, 15 Sep 2021 09:26:46 GMT

←Older revision		Revision as of 09:26, 15 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lus ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7lus' size='340' side='right'caption='[[7lus]]' scene=''>
-	~~Authors~~: ~~Sudom, A~~.~~, Whittington, D~~.~~, Garces, F~~.~~, Wang, Z~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~IgG2 Fc Charge Pair Mutation version 1 (CPMv1)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lus OCA], [https://pdbe.org/7lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lus RCSB], [https://www.ebi.ac.uk/pdbsum/7lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lus ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Whittington~~, D]]	+	__TOC__
-	[~~[Category~~: ~~Garces, F~~]]	+	</StructureSection>
-	[[Category: ~~Sudom, A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wang,~~ Z]]	+	[[Category: Z-disk]]

OCA at 06:11, 21 April 2021

OCA — Wed, 21 Apr 2021 06:11:46 GMT

←Older revision		Revision as of 06:11, 21 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lus is ON HOLD	+	The entry 7lus is ON HOLD until Paper Publication

	Authors: Sudom, A., Whittington, D., Garces, F., Wang, Z.		Authors: Sudom, A., Whittington, D., Garces, F., Wang, Z.
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	Description: IgG2 Fc Charge Pair Mutation version 1 (CPMv1)		Description: IgG2 Fc Charge Pair Mutation version 1 (CPMv1)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wang, Z]]
	[[Category: Whittington, D]]		[[Category: Whittington, D]]
	[[Category: Garces, F]]		[[Category: Garces, F]]
	[[Category: Sudom, A]]		[[Category: Sudom, A]]
		+	[[Category: Wang, Z]]

OCA: Protected "7lus" [edit=sysop:move=sysop]

OCA — Wed, 03 Mar 2021 13:27:40 GMT

Protected "7lus" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:27, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7lus is ON HOLD Authors: Sudom, A., Whittington, D., Garces, F., Wang, Z. Description: IgG2 Fc Charge Pair Mutation version 1 (CPMv1) [[Category: U...

OCA — Wed, 03 Mar 2021 13:27:38 GMT

New page: '''Unreleased structure''' The entry 7lus is ON HOLD Authors: Sudom, A., Whittington, D., Garces, F., Wang, Z. Description: IgG2 Fc Charge Pair Mutation version 1 (CPMv1) [[Category: U...

New page

'''Unreleased structure'''

The entry 7lus is ON HOLD

Authors: Sudom, A., Whittington, D., Garces, F., Wang, Z.

Description: IgG2 Fc Charge Pair Mutation version 1 (CPMv1)
[[Category: Unreleased Structures]]
[[Category: Wang, Z]]
[[Category: Whittington, D]]
[[Category: Garces, F]]
[[Category: Sudom, A]]