7lys - Revision history

OCA at 19:38, 29 May 2024

OCA — Wed, 29 May 2024 19:38:41 GMT

←Older revision		Revision as of 19:38, 29 May 2024
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-	====	+	==Cryo-EM structure of CasPhi-2 (Cas12j) bound to crRNA and DNA==
-	<StructureSection load='7lys' size='340' side='right'caption='[[7lys]]' scene=''>	+	<StructureSection load='7lys' size='340' side='right'caption='[[7lys]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7lys]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Biggievirus_Mos11 Biggievirus Mos11] and [https://en.wikipedia.org/wiki/Phage_#D Phage #D]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LYS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lys OCA], [https://pdbe.org/7lys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lys RCSB], [https://www.ebi.ac.uk/pdbsum/7lys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lys ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.05Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lys OCA], [https://pdbe.org/7lys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lys RCSB], [https://www.ebi.ac.uk/pdbsum/7lys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lys ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CRISPR-CasPhi, a small RNA-guided enzyme found uniquely in bacteriophages, achieves programmable DNA cutting as well as genome editing. To investigate how the hypercompact enzyme recognizes and cleaves double-stranded DNA, we determined cryo-EM structures of CasPhi (Cas12j) in pre- and post-DNA-binding states. The structures reveal a streamlined protein architecture that tightly encircles the CRISPR RNA and DNA target to capture, unwind and cleave DNA. Comparison of the pre- and post-DNA-binding states reveals how the protein rearranges for DNA cleavage upon target recognition. On the basis of these structures, we created and tested mutant forms of CasPhi that cut DNA up to 20-fold faster relative to wild type, showing how this system may be naturally attenuated to improve the fidelity of DNA interference. The structural and mechanistic insights into how CasPhi binds and cleaves DNA should allow for protein engineering for both in vitro diagnostics and genome editing.
		+
		+	DNA interference states of the hypercompact CRISPR-CasPhi effector.,Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA Nat Struct Mol Biol. 2021 Aug;28(8):652-661. doi: 10.1038/s41594-021-00632-3. , Epub 2021 Aug 11. PMID:34381246<ref>PMID:34381246</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7lys" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Biggievirus Mos11]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Phage #D]]
		+	[[Category: Doudna J]]
		+	[[Category: Nogales E]]
		+	[[Category: Pausch P]]
		+	[[Category: Soczek K]]

OCA at 10:20, 4 August 2021

OCA — Wed, 04 Aug 2021 10:20:12 GMT

←Older revision		Revision as of 10:20, 4 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7lys ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7lys' size='340' side='right'caption='[[7lys]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lys OCA], [https://pdbe.org/7lys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lys RCSB], [https://www.ebi.ac.uk/pdbsum/7lys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lys ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:21, 5 May 2021

OCA — Wed, 05 May 2021 07:21:35 GMT

←Older revision		Revision as of 07:21, 5 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7lys is ON HOLD	+	The entry 7lys is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7lys" [edit=sysop:move=sysop]

OCA — Wed, 17 Mar 2021 05:25:09 GMT

Protected "7lys" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 17 March 2021

OCA: New page: '''Unreleased structure''' The entry 7lys is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Mar 2021 05:25:08 GMT

New page: '''Unreleased structure''' The entry 7lys is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7lys is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]