OCA at 16:06, 18 October 2023

OCA — Wed, 18 Oct 2023 16:06:18 GMT

←Older revision		Revision as of 16:06, 18 October 2023
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-	====	+	==Room Temperature XFEL Crystallography reveals asymmetry in the vicinity of the two phylloquinones in Photosystem I==
-	<StructureSection load='7m76' size='340' side='right'caption='[[7m76]]' scene=''>	+	<StructureSection load='7m76' size='340' side='right'caption='[[7m76]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7m76]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m76 OCA], [https://pdbe.org/7m76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m76 RCSB], [https://www.ebi.ac.uk/pdbsum/7m76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m76 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL0:CHLOROPHYLL+A+ISOMER'>CL0</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=PQN:PHYLLOQUINONE'>PQN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m76 OCA], [https://pdbe.org/7m76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m76 RCSB], [https://www.ebi.ac.uk/pdbsum/7m76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m76 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PSAA_THEVB PSAA_THEVB] PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6.<ref>PMID:10066799</ref> <ref>PMID:10066800</ref> <ref>PMID:11418848</ref> <ref>PMID:8901876</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We have determined the structure of cyanobacterial PS I at room temperature (RT) using femtosecond X-ray pulses from an X-ray free electron laser (XFEL) that shows a clear expansion of the entire protein complex in the direction of the membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken at multiple XFEL beamlines. In the RT structure of PS I, we also observe conformational differences between the two branches in the reaction center around the secondary electron acceptors A(1A) and A(1B). The pi-stacked Phe residues are rotated with a more parallel orientation in the A-branch and an almost perpendicular confirmation in the B-branch, and the symmetry breaking PsaB-Trp673 is tilted and further away from A(1A). These changes increase the asymmetry between the branches and may provide insights into the preferential directionality of electron transfer.
		+
		+	Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I.,Keable SM, Kolsch A, Simon PS, Dasgupta M, Chatterjee R, Subramanian SK, Hussein R, Ibrahim M, Kim IS, Bogacz I, Makita H, Pham CC, Fuller FD, Gul S, Paley D, Lassalle L, Sutherlin KD, Bhowmick A, Moriarty NW, Young ID, Blaschke JP, de Lichtenberg C, Chernev P, Cheah MH, Park S, Park G, Kim J, Lee SJ, Park J, Tono K, Owada S, Hunter MS, Batyuk A, Oggenfuss R, Sander M, Zerdane S, Ozerov D, Nass K, Lemke H, Mankowsky R, Brewster AS, Messinger J, Sauter NK, Yachandra VK, Yano J, Zouni A, Kern J Sci Rep. 2021 Nov 8;11(1):21787. doi: 10.1038/s41598-021-00236-3. PMID:34750381<ref>PMID:34750381</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7m76" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Photosystem I 3D structures\|Photosystem I 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Thermosynechococcus vestitus BP-1]]
		+	[[Category: Keable SM]]
		+	[[Category: Kern J]]
		+	[[Category: Kolsch A]]
		+	[[Category: Simon PS]]
		+	[[Category: Yachandra VK]]
		+	[[Category: Yano J]]
		+	[[Category: Zouni A]]

OCA at 12:36, 24 November 2021

OCA — Wed, 24 Nov 2021 12:36:16 GMT

←Older revision		Revision as of 12:36, 24 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7m76 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7m76' size='340' side='right'caption='[[7m76]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m76 OCA], [https://pdbe.org/7m76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m76 RCSB], [https://www.ebi.ac.uk/pdbsum/7m76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m76 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:24, 8 September 2021

OCA — Wed, 08 Sep 2021 07:24:01 GMT

←Older revision		Revision as of 07:24, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7m76 is ON HOLD	+	The entry 7m76 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7m76" [edit=sysop:move=sysop]

OCA — Wed, 07 Apr 2021 04:36:57 GMT

Protected "7m76" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7m76 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Apr 2021 04:36:56 GMT

New page: '''Unreleased structure''' The entry 7m76 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7m76 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7m76 - Revision history

OCA at 16:06, 18 October 2023

OCA at 12:36, 24 November 2021

OCA at 07:24, 8 September 2021

OCA: Protected "7m76" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7m76 is ON HOLD Authors: Description: Category: Unreleased Structures