7mfz - Revision history

OCA at 16:10, 18 October 2023

OCA — Wed, 18 Oct 2023 16:10:41 GMT

←Older revision		Revision as of 16:10, 18 October 2023
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	==The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer==		==The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer==
-	<StructureSection load='7mfz' size='340' side='right'caption='[[7mfz]]' scene=''>	+	<StructureSection load='7mfz' size='340' side='right'caption='[[7mfz]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mfz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MFZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mfz OCA], [https://pdbe.org/7mfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mfz RCSB], [https://www.ebi.ac.uk/pdbsum/7mfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mfz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.49Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZFP:1-(4-{5-[(2E)-but-2-en-2-yl]thiophen-2-yl}phenyl)azetidine'>ZFP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mfz OCA], [https://pdbe.org/7mfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mfz RCSB], [https://www.ebi.ac.uk/pdbsum/7mfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mfz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN] Intracellular transport of retinol.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The incredible potential for fluorescent proteins to revolutionize biology has inspired the development of a variety of design strategies to address an equally broad range of photophysical characteristics, depending on potential applications. Of these, fluorescent proteins that simultaneously exhibit high quantum yield, red-shifted emission, and wide separation between excitation and emission wavelengths (Large Stokes Shift, LSS) are rare. The pursuit of LSS systems has led to the formation of a complex, obtained from the marriage of a rationally engineered protein (human cellular retinol binding protein II, hCRBPII) and different fluorogenic molecules, capable of supporting photobase activity. The large increase in basicity upon photoexcitation leads to protonation of the fluorophore in the excited state, dramatically red-shifting its emission, leading to an LSS protein/fluorophore complex. Essential for selective photobase activity is the intimate involvement of the target protein structure and sequence that enables Excited State Proton Transfer (ESPT). The potential power and usefulness of the strategy was demonstrated in live cell imaging of human cell lines.
		+
		+	Design of Large Stokes Shift Fluorescent Proteins Based on Excited State Proton Transfer of an Engineered Photobase.,Santos EM, Sheng W, Esmatpour Salmani R, Tahmasebi Nick S, Ghanbarpour A, Gholami H, Vasileiou C, Geiger JH, Borhan B J Am Chem Soc. 2021 Sep 22;143(37):15091-15102. doi: 10.1021/jacs.1c05039. Epub, 2021 Sep 13. PMID:34516091<ref>PMID:34516091</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mfz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Retinol-binding protein 3D structures\|Retinol-binding protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Geiger J]]		[[Category: Geiger J]]
	[[Category: Ghanbarpour A]]		[[Category: Ghanbarpour A]]

OCA at 09:57, 12 January 2022

OCA — Wed, 12 Jan 2022 09:57:20 GMT

←Older revision		Revision as of 09:57, 12 January 2022
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-	'''Unreleased structure'''

-	~~The entry 7mfz is ON HOLD until Paper Publication~~	+	==The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer==
-		+	<StructureSection load='7mfz' size='340' side='right'caption='[[7mfz]]' scene=''>
-	~~Authors: Ghanbarpour, A., Geiger, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MFZ FirstGlance]. <br>
-	~~Description:~~ The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mfz OCA], [https://pdbe.org/7mfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mfz RCSB], [https://www.ebi.ac.uk/pdbsum/7mfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mfz ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Ghanbarpour~~, A]]	+	__TOC__
-	[[Category: Geiger, J]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Geiger J]]
		+	[[Category: Ghanbarpour A]]

OCA at 08:51, 19 May 2021

OCA — Wed, 19 May 2021 08:51:58 GMT

←Older revision		Revision as of 08:51, 19 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mfz is ON HOLD	+	The entry 7mfz is ON HOLD until Paper Publication

	Authors: Ghanbarpour, A., Geiger, J.		Authors: Ghanbarpour, A., Geiger, J.

OCA: Protected "7mfz" [edit=sysop:move=sysop]

OCA — Wed, 21 Apr 2021 06:21:09 GMT

Protected "7mfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:21, 21 April 2021

OCA: New page: '''Unreleased structure''' The entry 7mfz is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Muta...

OCA — Wed, 21 Apr 2021 06:21:07 GMT

New page: '''Unreleased structure''' The entry 7mfz is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Muta...

New page

'''Unreleased structure'''

The entry 7mfz is ON HOLD

Authors: Ghanbarpour, A., Geiger, J.

Description: The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer
[[Category: Unreleased Structures]]
[[Category: Ghanbarpour, A]]
[[Category: Geiger, J]]