7mhr - Revision history

OCA at 16:11, 18 October 2023

OCA — Wed, 18 Oct 2023 16:11:49 GMT

←Older revision		Revision as of 16:11, 18 October 2023
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-	====	+	==KcsA E71V closed gate with K+==
-	<StructureSection load='7mhr' size='340' side='right'caption='[[7mhr]]' scene=''>	+	<StructureSection load='7mhr' size='340' side='right'caption='[[7mhr]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mhr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MHR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mhr OCA], [https://pdbe.org/7mhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mhr RCSB], [https://www.ebi.ac.uk/pdbsum/7mhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mhr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mhr OCA], [https://pdbe.org/7mhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mhr RCSB], [https://www.ebi.ac.uk/pdbsum/7mhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mhr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	C-type inactivation is of great physiological importance in voltage-activated K(+) channels (Kv), but its structural basis remains unresolved. Knowledge about C-type inactivation has been largely deduced from the bacterial K(+) channel KcsA, whose selectivity filter constricts under inactivating conditions. However, the filter is highly sensitive to its molecular environment, which is different in Kv channels than in KcsA. In particular, a glutamic acid residue at position 71 along the pore helix in KcsA is substituted by a valine conserved in most Kv channels, suggesting that this side chain is a molecular determinant of function. Here, a combination of X-ray crystallography, solid-state NMR and MD simulations of the E71V KcsA mutant is undertaken to explore inactivation in this Kv-like construct. X-ray and ssNMR data show that the filter of the Kv-like mutant does not constrict under inactivating conditions. Rather, the filter adopts a conformation that is slightly narrowed and rigidified. On the other hand, MD simulations indicate that the constricted conformation can nonetheless be stably established in the mutant channel. Together, these findings suggest that the Kv-like KcsA mutant may be associated with different modes of C-type inactivation, showing that distinct filter environments entail distinct C-type inactivation mechanisms.
		+
		+	A distinct mechanism of C-type inactivation in the Kv-like KcsA mutant E71V.,Rohaim A, Vermeulen BJA, Li J, Kummerer F, Napoli F, Blachowicz L, Medeiros-Silva J, Roux B, Weingarth M Nat Commun. 2022 Mar 23;13(1):1574. doi: 10.1038/s41467-022-28866-9. PMID:35322021<ref>PMID:35322021</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mhr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Streptomyces lividans]]
		+	[[Category: Li J]]
		+	[[Category: Rohaim A]]
		+	[[Category: Roux B]]
		+	[[Category: Weingarth M]]

OCA at 11:17, 23 March 2022

OCA — Wed, 23 Mar 2022 11:17:09 GMT

←Older revision		Revision as of 11:17, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7mhr ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7mhr' size='340' side='right'caption='[[7mhr]]' scene=''>
-	~~Authors~~: ~~Rohaim, A~~.~~, Li, J~~.~~, Weingarth, M~~.~~, Roux, B~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~KcsA E71V closed gate with K+~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mhr OCA], [https://pdbe.org/7mhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mhr RCSB], [https://www.ebi.ac.uk/pdbsum/7mhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mhr ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: Li, J]]	+	__TOC__
-	[~~[Category~~: ~~Roux, B~~]]	+	</StructureSection>
-	[[Category: ~~Weingarth, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Rohaim, A~~]]	+	[[Category: Z-disk]]

OCA at 04:18, 28 April 2021

OCA — Wed, 28 Apr 2021 04:18:21 GMT

←Older revision		Revision as of 04:18, 28 April 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mhr is ON HOLD	+	The entry 7mhr is ON HOLD until Paper Publication

	Authors: Rohaim, A., Li, J., Weingarth, M., Roux, B.		Authors: Rohaim, A., Li, J., Weingarth, M., Roux, B.

-	Description: KcsA E71V ~~mutant,~~ closed gate with K+	+	Description: KcsA E71V closed gate with K+
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Li, J]]		[[Category: Li, J]]
	[[Category: Roux, B]]		[[Category: Roux, B]]
-	[[Category: Rohaim, A]]
	[[Category: Weingarth, M]]		[[Category: Weingarth, M]]
		+	[[Category: Rohaim, A]]

OCA: Protected "7mhr" [edit=sysop:move=sysop]

OCA — Wed, 21 Apr 2021 06:27:18 GMT

Protected "7mhr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 21 April 2021

OCA: New page: '''Unreleased structure''' The entry 7mhr is ON HOLD Authors: Rohaim, A., Li, J., Weingarth, M., Roux, B. Description: KcsA E71V mutant, closed gate with K+ [[Category: Unreleased Stru...

OCA — Wed, 21 Apr 2021 06:27:16 GMT

New page: '''Unreleased structure''' The entry 7mhr is ON HOLD Authors: Rohaim, A., Li, J., Weingarth, M., Roux, B. Description: KcsA E71V mutant, closed gate with K+ [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 7mhr is ON HOLD

Authors: Rohaim, A., Li, J., Weingarth, M., Roux, B.

Description: KcsA E71V mutant, closed gate with K+
[[Category: Unreleased Structures]]
[[Category: Li, J]]
[[Category: Roux, B]]
[[Category: Rohaim, A]]
[[Category: Weingarth, M]]