7mpy - Revision history

OCA at 16:18, 18 October 2023

OCA — Wed, 18 Oct 2023 16:18:22 GMT

←Older revision		Revision as of 16:18, 18 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH]] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref>	+	[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref>

	==See Also==		==See Also==

OCA at 05:22, 8 September 2022

OCA — Thu, 08 Sep 2022 05:22:57 GMT

←Older revision		Revision as of 05:22, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH]] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref>	+	[[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH]] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Herbicides are vital for modern agriculture, but their utility is threatened by genetic or metabolic resistance in weeds, as well as regulatory barriers. Of the known herbicide modes of action, 7,8-dihydropterin synthase (DHPS), which is involved in folate biosynthesis, is targeted by just one commercial herbicide, asulam. A mimic of the substrate para-aminobenzoic acid, asulam is chemically similar to sulfonamide antibiotics, and although it is still in widespread use, asulam has faced regulatory scrutiny. With an entire mode of action represented by just one commercial agrochemical, we sought to improve the understanding of its plant target. Here we solve a 2.3 A resolution crystal structure for Arabidopsis thaliana DHPS that is conjoined to 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), and we reveal a strong structural conservation with bacterial counterparts at the sulfonamide-binding pocket of DHPS. We demonstrate that asulam and the antibiotic sulfamethoxazole have herbicidal as well as antibacterial activity, and we explore the structural basis of their potency by modeling these compounds in mitochondrial HPPK/DHPS. Our findings suggest limited opportunity for the rational design of plant selectivity from asulam and indicate that pharmacokinetic or delivery differences between plants and microbes might be the best ways to safeguard this mode of action.	+

-	Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam.,Vadlamani G, Sukhoverkov KV, Haywood J, Breese KJ, Fisher MF, Stubbs KA, Bond CS, Mylne JS Plant Commun. 2022 Apr 9:100322. doi: 10.1016/j.xplc.2022.100322. PMID:35605193<ref>PMID:35605193</ref>	+	==See Also==
-		+	*[[HPPK 3D structures\|HPPK 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 7mpy" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bond~~, C S~~]]	+	[[Category: Bond CS]]
-	[[Category: Vadlamani, G]]	+	[[Category: Vadlamani G]]
-	~~[[Category: Folate biosynthesis]]~~	+
-	~~[[Category: Herbicide target]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:35, 8 June 2022

OCA — Wed, 08 Jun 2022 05:35:06 GMT

←Older revision		Revision as of 05:35, 8 June 2022
Line 1:		Line 1:

	==Crystal structure of cytosolic HPPK-DHPS from A.thaliana==		==Crystal structure of cytosolic HPPK-DHPS from A.thaliana==
-	<StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]]' scene=''>	+	<StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH]] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Herbicides are vital for modern agriculture, but their utility is threatened by genetic or metabolic resistance in weeds, as well as regulatory barriers. Of the known herbicide modes of action, 7,8-dihydropterin synthase (DHPS), which is involved in folate biosynthesis, is targeted by just one commercial herbicide, asulam. A mimic of the substrate para-aminobenzoic acid, asulam is chemically similar to sulfonamide antibiotics, and although it is still in widespread use, asulam has faced regulatory scrutiny. With an entire mode of action represented by just one commercial agrochemical, we sought to improve the understanding of its plant target. Here we solve a 2.3 A resolution crystal structure for Arabidopsis thaliana DHPS that is conjoined to 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), and we reveal a strong structural conservation with bacterial counterparts at the sulfonamide-binding pocket of DHPS. We demonstrate that asulam and the antibiotic sulfamethoxazole have herbicidal as well as antibacterial activity, and we explore the structural basis of their potency by modeling these compounds in mitochondrial HPPK/DHPS. Our findings suggest limited opportunity for the rational design of plant selectivity from asulam and indicate that pharmacokinetic or delivery differences between plants and microbes might be the best ways to safeguard this mode of action.
		+
		+	Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam.,Vadlamani G, Sukhoverkov KV, Haywood J, Breese KJ, Fisher MF, Stubbs KA, Bond CS, Mylne JS Plant Commun. 2022 Apr 9:100322. doi: 10.1016/j.xplc.2022.100322. PMID:35605193<ref>PMID:35605193</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mpy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bond CS]]	+	[[Category: Bond, C S]]
-	[[Category: Vadlamani G]]	+	[[Category: Vadlamani, G]]
		+	[[Category: Folate biosynthesis]]
		+	[[Category: Herbicide target]]
		+	[[Category: Plant protein]]
		+	[[Category: Transferase]]

OCA at 09:55, 8 December 2021

OCA — Wed, 08 Dec 2021 09:55:54 GMT

←Older revision		Revision as of 09:55, 8 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7mpy ~~is ON HOLD~~	+	==Crystal structure of cytosolic HPPK-DHPS from A.thaliana==
-		+	<StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bond CS]]
		+	[[Category: Vadlamani G]]

OCA at 04:59, 1 December 2021

OCA — Wed, 01 Dec 2021 04:59:28 GMT

←Older revision		Revision as of 04:59, 1 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mpy is ON HOLD ~~until Paper Publication~~	+	The entry 7mpy is ON HOLD

	Authors:		Authors:

OCA at 08:54, 19 May 2021

OCA — Wed, 19 May 2021 08:54:42 GMT

←Older revision		Revision as of 08:54, 19 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mpy is ON HOLD	+	The entry 7mpy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7mpy" [edit=sysop:move=sysop]

OCA — Wed, 12 May 2021 08:15:45 GMT

Protected "7mpy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:15, 12 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mpy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 May 2021 08:15:44 GMT

New page: '''Unreleased structure''' The entry 7mpy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7mpy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]