7mse - Revision history

OCA at 16:19, 18 October 2023

OCA — Wed, 18 Oct 2023 16:19:39 GMT

←Older revision		Revision as of 16:19, 18 October 2023
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	<StructureSection load='7mse' size='340' side='right'caption='[[7mse]], [[Resolution\|resolution]] 1.27Å' scene=''>		<StructureSection load='7mse' size='340' side='right'caption='[[7mse]], [[Resolution\|resolution]] 1.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7mse]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mse]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TLA:L(+)~~-~~TARTARIC+ACID'>TLA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.27Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>DDT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/D-dopachrome_decarboxylase D-dopachrome decarboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.84 4.1.1.84]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mse OCA], [https://pdbe.org/7mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mse RCSB], [https://www.ebi.ac.uk/pdbsum/7mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mse OCA], [https://pdbe.org/7mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mse RCSB], [https://www.ebi.ac.uk/pdbsum/7mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DOPD_HUMAN DOPD_HUMAN]] Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).	+	[https://www.uniprot.org/uniprot/DOPD_HUMAN DOPD_HUMAN] Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~D-dopachrome decarboxylase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lolis, E]]	+	[[Category: Lolis E]]
-	[[Category: Manjula, R]]	+	[[Category: Manjula R]]
-	[[Category: Murphy~~, E L~~]]	+	[[Category: Murphy EL]]
-	[[Category: Murphy~~, J W]]~~	+	[[Category: Murphy JW]]
-	~~[[Category: Cytokine~~]]	+

OCA at 06:35, 25 August 2021

OCA — Wed, 25 Aug 2021 06:35:40 GMT

←Older revision		Revision as of 06:35, 25 August 2021
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	==High-resolution crystal structure of hMIF2 with tartrate at the active site==		==High-resolution crystal structure of hMIF2 with tartrate at the active site==
-	<StructureSection load='7mse' size='340' side='right'caption='[[7mse]]' scene=''>	+	<StructureSection load='7mse' size='340' side='right'caption='[[7mse]], [[Resolution\|resolution]] 1.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mse]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mse OCA], [https://pdbe.org/7mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mse RCSB], [https://www.ebi.ac.uk/pdbsum/7mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mse ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/D-dopachrome_decarboxylase D-dopachrome decarboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.84 4.1.1.84] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mse OCA], [https://pdbe.org/7mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mse RCSB], [https://www.ebi.ac.uk/pdbsum/7mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mse ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/DOPD_HUMAN DOPD_HUMAN]] Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The MIF family of cytokines contain multiple ligand binding sites and mediate immunomodulatory processes through an undefined mechanism(s). Previously, we reported a dynamic relay connecting the MIF catalytic site to an allosteric site at its solvent channel. Despite structural and functional similarity, the MIF homolog D-dopachrome tautomerase (also called MIF-2) has low sequence identity (35%), prompting the question of whether this dynamic regulatory network is conserved. Here, we establish the structural basis of an allosteric site in MIF-2, showing with solution NMR that dynamic communication is preserved in MIF-2 despite differences in the primary sequence. X-ray crystallography and NMR detail the structural consequences of perturbing residues in this pathway, which include conformational changes surrounding the allosteric site, despite global preservation of the MIF-2 fold. Molecular simulations reveal MIF-2 to contain a comparable hydrogen bond network to that of MIF, which was previously hypothesized to influence catalytic activity by modulating the strength of allosteric coupling. Disruption of the allosteric relay by mutagenesis also attenuates MIF-2 enzymatic activity in vitro and the activation of the CD74 receptor in vivo, highlighting a conserved point of control for non-overlapping functions in the MIF superfamily.
		+
		+	A Structurally Preserved Allosteric Site in the MIF Superfamily Affects Enzymatic Activity and CD74 Activation in D-dopachrome Tautomerase.,Chen E, Reiss K, Shah D, Manjula R, Allen B, Murphy EL, Murphy JW, Batista VS, Bhandari V, Lolis EJ, Lisi GP J Biol Chem. 2021 Aug 9:101061. doi: 10.1016/j.jbc.2021.101061. PMID:34384784<ref>PMID:34384784</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mse" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: D-dopachrome decarboxylase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lolis E]]	+	[[Category: Lolis, E]]
-	[[Category: Manjula R]]	+	[[Category: Manjula, R]]
-	[[Category: Murphy EL]]	+	[[Category: Murphy, E L]]
-	[[Category: Murphy JW]]	+	[[Category: Murphy, J W]]
		+	[[Category: Cytokine]]

OCA at 06:08, 18 August 2021

OCA — Wed, 18 Aug 2021 06:08:30 GMT

←Older revision		Revision as of 06:08, 18 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mse ~~is ON HOLD until Paper Publication~~	+	==High-resolution crystal structure of hMIF2 with tartrate at the active site==
-		+	<StructureSection load='7mse' size='340' side='right'caption='[[7mse]]' scene=''>
-	~~Authors~~: ~~Murphy~~, E.L., ~~Manjula~~, R., ~~Murphy, J~~.W., ~~Lolis, E~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MSE FirstGlance]. <br>
-	~~Description: High-resolution crystal structure of hMIF2 with tartrate at the active site~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mse OCA], [https://pdbe.org/7mse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mse RCSB], [https://www.ebi.ac.uk/pdbsum/7mse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mse ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Manjula, R~~]]	+	__TOC__
-	[[Category: ~~Murphy, J.W~~]]	+	</StructureSection>
-	[[Category: Murphy~~, E.L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lolis, E~~]]	+	[[Category: Lolis E]]
		+	[[Category: Manjula R]]
		+	[[Category: Murphy EL]]
		+	[[Category: Murphy JW]]

OCA at 11:05, 9 June 2021

OCA — Wed, 09 Jun 2021 11:05:42 GMT

←Older revision		Revision as of 11:05, 9 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mse is ON HOLD	+	The entry 7mse is ON HOLD until Paper Publication

	Authors: Murphy, E.L., Manjula, R., Murphy, J.W., Lolis, E.		Authors: Murphy, E.L., Manjula, R., Murphy, J.W., Lolis, E.

-	Description: ~~Crystal~~ structure of ~~MIF2 (D-DT)~~	+	Description: High-resolution crystal structure of hMIF2 with tartrate at the active site
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Manjula, R]]		[[Category: Manjula, R]]

OCA: Protected "7mse" [edit=sysop:move=sysop]

OCA — Wed, 19 May 2021 08:57:33 GMT

Protected "7mse" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:57, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mse is ON HOLD Authors: Murphy, E.L., Manjula, R., Murphy, J.W., Lolis, E. Description: Crystal structure of MIF2 (D-DT) [[Category: Unreleased St...

OCA — Wed, 19 May 2021 08:57:32 GMT

New page: '''Unreleased structure''' The entry 7mse is ON HOLD Authors: Murphy, E.L., Manjula, R., Murphy, J.W., Lolis, E. Description: Crystal structure of MIF2 (D-DT) [[Category: Unreleased St...

New page

'''Unreleased structure'''

The entry 7mse is ON HOLD

Authors: Murphy, E.L., Manjula, R., Murphy, J.W., Lolis, E.

Description: Crystal structure of MIF2 (D-DT)
[[Category: Unreleased Structures]]
[[Category: Manjula, R]]
[[Category: Murphy, J.W]]
[[Category: Murphy, E.L]]
[[Category: Lolis, E]]