7mtl - Revision history

OCA at 16:19, 18 October 2023

OCA — Wed, 18 Oct 2023 16:19:59 GMT

←Older revision		Revision as of 16:19, 18 October 2023
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-	====	+	==Crystal structure of colibactin self-resistance protein ClbS in complex with a dsDNA==
-	<StructureSection load='7mtl' size='340' side='right'caption='[[7mtl]]' scene=''>	+	<StructureSection load='7mtl' size='340' side='right'caption='[[7mtl]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mtl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MTL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mtl OCA], [https://pdbe.org/7mtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mtl RCSB], [https://www.ebi.ac.uk/pdbsum/7mtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mtl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.446Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mtl OCA], [https://pdbe.org/7mtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mtl RCSB], [https://www.ebi.ac.uk/pdbsum/7mtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mtl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0P7K8_ECOLX Q0P7K8_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The natural product colibactin, along with its associated biosynthetic gene cluster, is an example system for the role microbially derived small molecules play in the human microbiome. This is particularly relevant in the human gut, where host microbiota is involved in various disorders, including colorectal cancer pathogenesis. Bacteria harboring the colibactin gene cluster induce alkylation of nucleobases in host DNA, forming interstrand cross-links both in vivo and in vitro. These lesions can lead to deleterious double-strand breaks and have been identified as the primary mechanism of colibactin-induced cytotoxicity. The gene product ClbS is one of several mechanisms utilized by the producing bacteria to maintain genome integrity. ClbS catalyzes hydrolytic inactivation of colibactin and has been shown to bind DNA, incurring self-resistance. Presented is the molecular basis for ClbS bound to a DNA oligonucleotide. The structure shows the interaction of the protein with the ends of a DNA duplex with terminal nucleotides flipped to the enzyme active site. The structure suggests an additional function for ClbS, the binding to damaged DNA followed by repair. Additionally, our study provides general insight into the function of the widely distributed and largely uncharacterized DUF1706 protein family.
		+
		+	Structural Basis for the Interactions of the Colibactin Resistance Gene Product ClbS with DNA.,Tripathi P, Bruner SD Biochemistry. 2021 May 25;60(20):1619-1625. doi: 10.1021/acs.biochem.1c00201., Epub 2021 May 4. PMID:33945270<ref>PMID:33945270</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mtl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Bruner SD]]
		+	[[Category: Tripathi P]]

OCA at 09:21, 26 May 2021

OCA — Wed, 26 May 2021 09:21:10 GMT

←Older revision		Revision as of 09:21, 26 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mtl ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7mtl' size='340' side='right'caption='[[7mtl]]' scene=''>
-	~~Authors~~: ~~Tripathi, P~~.~~, Bruner, S~~.D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of colibactin self~~-~~resistance protein ClbS in complex with~~ a ~~dsDNA~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mtl OCA], [https://pdbe.org/7mtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mtl RCSB], [https://www.ebi.ac.uk/pdbsum/7mtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mtl ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Tripathi, P~~]]	+	__TOC__
-	[[Category: ~~Bruner, S.D~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7mtl" [edit=sysop:move=sysop]

OCA — Wed, 19 May 2021 08:59:50 GMT

Protected "7mtl" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:59, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mtl is ON HOLD Authors: Tripathi, P., Bruner, S.D. Description: Crystal structure of colibactin self-resistance protein ClbS in complex with a dsD...

OCA — Wed, 19 May 2021 08:59:49 GMT

New page: '''Unreleased structure''' The entry 7mtl is ON HOLD Authors: Tripathi, P., Bruner, S.D. Description: Crystal structure of colibactin self-resistance protein ClbS in complex with a dsD...

New page

'''Unreleased structure'''

The entry 7mtl is ON HOLD

Authors: Tripathi, P., Bruner, S.D.

Description: Crystal structure of colibactin self-resistance protein ClbS in complex with a dsDNA
[[Category: Unreleased Structures]]
[[Category: Tripathi, P]]
[[Category: Bruner, S.D]]