OCA at 16:20, 18 October 2023

OCA — Wed, 18 Oct 2023 16:20:44 GMT

←Older revision		Revision as of 16:20, 18 October 2023
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-	====	+	==Structure of hepatitis C virus envelope full-length glycoprotein 2 (eE2) from J6 genotype==
-	<StructureSection load='7mww' size='340' side='right'caption='[[7mww]]' scene=''>	+	<StructureSection load='7mww' size='340' side='right'caption='[[7mww]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mww]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepacivirus_C Hepacivirus C] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mww OCA], [https://pdbe.org/7mww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mww RCSB], [https://www.ebi.ac.uk/pdbsum/7mww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mww ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.71Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mww OCA], [https://pdbe.org/7mww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mww RCSB], [https://www.ebi.ac.uk/pdbsum/7mww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mww ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2I6PIY9_9HEPC A0A2I6PIY9_9HEPC] RNA-dependent RNA polymerase that performs primer-template recognition and RNA synthesis during viral replication.[ARBA:ARBA00023584]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus (HCV) infection is a causal agent of chronic liver disease, cirrhosis and hepatocellular carcinoma in humans, and afflicts more than 70 million people worldwide. The HCV envelope glycoproteins E1 and E2 are responsible for the binding of the virus to the host cell, but the exact entry process remains undetermined(1). The majority of broadly neutralizing antibodies block interaction between HCV E2 and the large extracellular loop (LEL) of the cellular receptor CD81 (CD81-LEL)(2). Here we show that low pH enhances the binding of CD81-LEL to E2, and we determine the crystal structure of E2 in complex with an antigen-binding fragment (2A12) and CD81-LEL (E2-2A12-CD81-LEL); E2 in complex with 2A12 (E2-2A12); and CD81-LEL alone. After binding CD81, residues 418-422 in E2 are displaced, which allows for the extension of an internal loop consisting of residues 520-539. Docking of the E2-CD81-LEL complex onto a membrane-embedded, full-length CD81 places the residues Tyr529 and Trp531 of E2 proximal to the membrane. Liposome flotation assays show that low pH and CD81-LEL increase the interaction of E2 with membranes, whereas structure-based mutants of Tyr529, Trp531 and Ile422 in the amino terminus of E2 abolish membrane binding. These data support a model in which acidification and receptor binding result in a conformational change in E2 in preparation for membrane fusion.
		+
		+	Structural insights into hepatitis C virus receptor binding and entry.,Kumar A, Hossain RA, Yost SA, Bu W, Wang Y, Dearborn AD, Grakoui A, Cohen JI, Marcotrigiano J Nature. 2021 Sep 15. pii: 10.1038/s41586-021-03913-5. doi:, 10.1038/s41586-021-03913-5. PMID:34526719<ref>PMID:34526719</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mww" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hepacivirus C]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Bu W]]
		+	[[Category: Cohen JI]]
		+	[[Category: Dearborn AD]]
		+	[[Category: Grakoui A]]
		+	[[Category: Hossain RA]]
		+	[[Category: Kumar A]]
		+	[[Category: Marcotrigiano J]]
		+	[[Category: Wang Y]]
		+	[[Category: Yost SA]]

OCA at 09:27, 15 September 2021

OCA — Wed, 15 Sep 2021 09:27:33 GMT

←Older revision		Revision as of 09:27, 15 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mww ~~is ON HOLD until May 17 2023~~	+	====
-		+	<StructureSection load='7mww' size='340' side='right'caption='[[7mww]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mww OCA], [https://pdbe.org/7mww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mww RCSB], [https://www.ebi.ac.uk/pdbsum/7mww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mww ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 13:28, 2 June 2021

OCA — Wed, 02 Jun 2021 13:28:47 GMT

←Older revision		Revision as of 13:28, 2 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mww is ON HOLD	+	The entry 7mww is ON HOLD until May 17 2023

	Authors:		Authors:

OCA: Protected "7mww" [edit=sysop:move=sysop]

OCA — Wed, 26 May 2021 08:01:14 GMT

Protected "7mww" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:01, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mww is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 May 2021 08:01:13 GMT

New page: '''Unreleased structure''' The entry 7mww is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7mww is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7mww - Revision history

OCA at 16:20, 18 October 2023

OCA at 09:27, 15 September 2021

OCA at 13:28, 2 June 2021

OCA: Protected "7mww" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mww is ON HOLD Authors: Description: Category: Unreleased Structures