7mwy - Revision history

OCA at 07:19, 3 April 2024

OCA — Wed, 03 Apr 2024 07:19:18 GMT

←Older revision		Revision as of 07:19, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='7mwy' size='340' side='right'caption='[[7mwy]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='7mwy' size='340' side='right'caption='[[7mwy]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7mwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Droeu Droeu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_eugracilis Drosophila eugracilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mwy OCA], [https://pdbe.org/7mwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mwy RCSB], [https://www.ebi.ac.uk/pdbsum/7mwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mwy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mwy OCA], [https://pdbe.org/7mwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mwy RCSB], [https://www.ebi.ac.uk/pdbsum/7mwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mwy ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/D9IEF7_BPT4 D9IEF7_BPT4]
-	~~Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that produces the second messenger 2'3'-cGAMP and controls activation of innate immunity in mammalian cells(1-5)~~. ~~Animal genomes typically encode multiple proteins with predicted homology to cGAS(6-10), but the function of these uncharacterized enzymes is unknown~~. Here we show that cGAS-like receptors (cGLRs) are innate immune sensors capable of recognizing divergent molecular patterns and catalyzing synthesis of distinct nucleotide second messenger signals. Crystal structures of human and insect cGLRs reveal a nucleotidyltransferase signaling core shared with cGAS and a diversified primary ligand-binding surface modified with significant insertions and deletions. We demonstrate that cGLR surface remodeling enables altered ligand specificity and use a forward biochemical screen to identify cGLR1 as a double-stranded RNA sensor in the model organism Drosophila melanogaster. Surprisingly, RNA recognition activates Drosophila cGLR1 to synthesize the novel product cG[3'-5']pA[2'-5']p (3'2'-cGAMP). A crystal structure of Drosophila Stimulator of Interferon Genes (STING) in complex with 3'2'-cGAMP explains selective isomer recognition and we demonstrate that 3'2'-cGAMP induces an enhanced antiviral state in vivo that protects from viral infection. Similar to radiation of Toll-like receptors in pathogen immunity, our results establish cGLRs as a diverse family of metazoan pattern recognition receptors.	+

-	cGAS-like receptors sense RNA and control 3'2'-cGAMP signaling in Drosophila.,Slavik KM, Morehouse BR, Ragucci AE, Zhou W, Ai X, Chen Y, Li L, Wei Z, Bahre H, Konig M, Seifert R, Lee ASY, Cai H, Imler JL, Kranzusch PJ Nature. 2021 Jul 14. pii: 10.1038/s41586-021-03743-5. doi:, 10.1038/s41586-021-03743-5. PMID:34261127<ref>PMID:34261127</ref>	+	==See Also==
-		+	*[[Stimulator of interferon genes protein 3D structures\|Stimulator of interferon genes protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 7mwy" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Droeu~~]]	+	[[Category: Drosophila eugracilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kranzusch~~, P J~~]]	+	[[Category: Kranzusch PJ]]
-	[[Category: Ragucci~~, A E~~]]	+	[[Category: Ragucci AE]]
-	[[Category: Slavik~~, K M]]~~	+	[[Category: Slavik KM]]
-	~~[[Category: Cyclic dinucleotide receptor]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Sting~~]]	+

OCA at 10:56, 28 July 2021

OCA — Wed, 28 Jul 2021 10:56:10 GMT

←Older revision		Revision as of 10:56, 28 July 2021
Line 1:		Line 1:

	==Structure of the drosophila STING cyclic dinucleotide binding domain==		==Structure of the drosophila STING cyclic dinucleotide binding domain==
-	<StructureSection load='7mwy' size='340' side='right'caption='[[7mwy]]' scene=''>	+	<StructureSection load='7mwy' size='340' side='right'caption='[[7mwy]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Droeu Droeu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mwy OCA], [https://pdbe.org/7mwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mwy RCSB], [https://www.ebi.ac.uk/pdbsum/7mwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mwy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mwy OCA], [https://pdbe.org/7mwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mwy RCSB], [https://www.ebi.ac.uk/pdbsum/7mwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mwy ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that produces the second messenger 2'3'-cGAMP and controls activation of innate immunity in mammalian cells(1-5). Animal genomes typically encode multiple proteins with predicted homology to cGAS(6-10), but the function of these uncharacterized enzymes is unknown. Here we show that cGAS-like receptors (cGLRs) are innate immune sensors capable of recognizing divergent molecular patterns and catalyzing synthesis of distinct nucleotide second messenger signals. Crystal structures of human and insect cGLRs reveal a nucleotidyltransferase signaling core shared with cGAS and a diversified primary ligand-binding surface modified with significant insertions and deletions. We demonstrate that cGLR surface remodeling enables altered ligand specificity and use a forward biochemical screen to identify cGLR1 as a double-stranded RNA sensor in the model organism Drosophila melanogaster. Surprisingly, RNA recognition activates Drosophila cGLR1 to synthesize the novel product cG[3'-5']pA[2'-5']p (3'2'-cGAMP). A crystal structure of Drosophila Stimulator of Interferon Genes (STING) in complex with 3'2'-cGAMP explains selective isomer recognition and we demonstrate that 3'2'-cGAMP induces an enhanced antiviral state in vivo that protects from viral infection. Similar to radiation of Toll-like receptors in pathogen immunity, our results establish cGLRs as a diverse family of metazoan pattern recognition receptors.
		+
		+	cGAS-like receptors sense RNA and control 3'2'-cGAMP signaling in Drosophila.,Slavik KM, Morehouse BR, Ragucci AE, Zhou W, Ai X, Chen Y, Li L, Wei Z, Bahre H, Konig M, Seifert R, Lee ASY, Cai H, Imler JL, Kranzusch PJ Nature. 2021 Jul 14. pii: 10.1038/s41586-021-03743-5. doi:, 10.1038/s41586-021-03743-5. PMID:34261127<ref>PMID:34261127</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mwy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Droeu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kranzusch PJ]]	+	[[Category: Kranzusch, P J]]
-	[[Category: Ragucci AE]]	+	[[Category: Ragucci, A E]]
-	[[Category: Slavik KM]]	+	[[Category: Slavik, K M]]
		+	[[Category: Cyclic dinucleotide receptor]]
		+	[[Category: Immune system]]
		+	[[Category: Sting]]

OCA at 08:58, 21 July 2021

OCA — Wed, 21 Jul 2021 08:58:52 GMT

←Older revision		Revision as of 08:58, 21 July 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7mwy is ~~ON HOLD until Paper Publication~~	+	==Structure of the drosophila STING cyclic dinucleotide binding domain==
-		+	<StructureSection load='7mwy' size='340' side='right'caption='[[7mwy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MWY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mwy OCA], [https://pdbe.org/7mwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mwy RCSB], [https://www.ebi.ac.uk/pdbsum/7mwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mwy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kranzusch PJ]]
		+	[[Category: Ragucci AE]]
		+	[[Category: Slavik KM]]

OCA at 13:28, 2 June 2021

OCA — Wed, 02 Jun 2021 13:28:49 GMT

←Older revision		Revision as of 13:28, 2 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mwy is ON HOLD	+	The entry 7mwy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7mwy" [edit=sysop:move=sysop]

OCA — Wed, 26 May 2021 08:01:19 GMT

Protected "7mwy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:01, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mwy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 May 2021 08:01:18 GMT

New page: '''Unreleased structure''' The entry 7mwy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7mwy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]