OCA at 16:21, 18 October 2023

OCA — Wed, 18 Oct 2023 16:21:09 GMT

←Older revision		Revision as of 16:21, 18 October 2023
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-	====	+	==IgE-Fc in complex with DARPins E2_79 and E3_53==
-	<StructureSection load='7mxi' size='340' side='right'caption='[[7mxi]]' scene=''>	+	<StructureSection load='7mxi' size='340' side='right'caption='[[7mxi]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mxi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MXI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mxi OCA], [https://pdbe.org/7mxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mxi RCSB], [https://www.ebi.ac.uk/pdbsum/7mxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mxi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mxi OCA], [https://pdbe.org/7mxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mxi RCSB], [https://www.ebi.ac.uk/pdbsum/7mxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mxi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Anaphylaxis represents one of the most severe and fatal forms of allergic reactions. Like most other allergies, it is caused by activation of basophils and mast cells by allergen-mediated cross-linking of IgE bound to its high-affinity receptor, FcepsilonRI, on the cell surface. The systemic release of soluble mediators induces an inflammatory cascade, rapidly causing symptoms with peak severity in minutes to hours after allergen exposure. Primary treatment for anaphylaxis consists of immediate intramuscular administration of adrenaline. OBJECTIVE: While adrenaline alleviates life-threatening symptoms of an anaphylactic reaction, there are currently no disease-modifying interventions available. We sought to develop potent and fast-acting IgE inhibitors with the potential to rapidly terminate acute allergic reactions. METHODS: Using affinity maturation by yeast display and structure-guided molecular engineering, we generated 3 optimized disruptive IgE inhibitors based on designed ankyrin repeat proteins and assessed their ability to actively remove IgE from allergic effector cells in vitro as well as in vivo in mice. RESULTS: The engineered IgE inhibitors rapidly dissociate preformed IgE:FcepsilonRI complexes, terminate IgE-mediated signaling in preactivated human blood basophils in vitro, and shut down preinitiated allergic reactions and anaphylaxis in mice in vivo. CONCLUSIONS: Fast-acting disruptive IgE inhibitors demonstrate the feasibility of developing kinetically optimized inhibitors for the treatment of anaphylaxis and the rapid desensitization of allergic individuals.
		+
		+	Structure-guided design of ultrapotent disruptive IgE inhibitors to rapidly terminate acute allergic reactions.,Pennington LF, Gasser P, Brigger D, Guntern P, Eggel A, Jardetzky TS J Allergy Clin Immunol. 2021 Oct;148(4):1049-1060. doi: , 10.1016/j.jaci.2021.03.050. Epub 2021 May 12. PMID:33991582<ref>PMID:33991582</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mxi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Jardetzky TJ]]
		+	[[Category: Pennington LF]]

OCA at 10:35, 28 July 2021

OCA — Wed, 28 Jul 2021 10:35:18 GMT

←Older revision		Revision as of 10:35, 28 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mxi ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7mxi' size='340' side='right'caption='[[7mxi]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mxi OCA], [https://pdbe.org/7mxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mxi RCSB], [https://www.ebi.ac.uk/pdbsum/7mxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mxi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:44, 7 July 2021

OCA — Wed, 07 Jul 2021 08:44:47 GMT

←Older revision		Revision as of 08:44, 7 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mxi is ON HOLD	+	The entry 7mxi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7mxi" [edit=sysop:move=sysop]

OCA — Wed, 26 May 2021 08:02:13 GMT

Protected "7mxi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:02, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7mxi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 May 2021 08:02:12 GMT

New page: '''Unreleased structure''' The entry 7mxi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7mxi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7mxi - Revision history

OCA at 16:21, 18 October 2023

OCA at 10:35, 28 July 2021

OCA at 08:44, 7 July 2021

OCA: Protected "7mxi" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mxi is ON HOLD Authors: Description: Category: Unreleased Structures