7mz6 - Revision history

OCA at 12:55, 13 October 2021

OCA — Wed, 13 Oct 2021 12:55:04 GMT

←Older revision		Revision as of 12:55, 13 October 2021
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	==Cryo-EM structure of minimal TRPV1 with 1 perturbed PI==		==Cryo-EM structure of minimal TRPV1 with 1 perturbed PI==
-	<StructureSection load='7mz6' size='340' side='right'caption='[[7mz6]]' scene=''>	+	<StructureSection load='7mz6' size='340' side='right'caption='[[7mz6]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mz6]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mz6 OCA], [https://pdbe.org/7mz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7mz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mz6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6IY:(10R,13S)-16-amino-13-hydroxy-7,13-dioxo-8,12,14-trioxa-13lambda~5~-phosphahexadecan-10-yl+hexadecanoate'>6IY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=XJ7:(2S)-1-(butanoyloxy)-3-{[(R)-hydroxy{[(1r,2R,3S,4S,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propan-2-yl+tridecanoate'>XJ7</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mz6 OCA], [https://pdbe.org/7mz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7mz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mz6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TRPV1_RAT TRPV1_RAT]] Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.<ref>PMID:9349813</ref> <ref>PMID:10644739</ref> <ref>PMID:11140687</ref> <ref>PMID:11418861</ref> <ref>PMID:12095983</ref> <ref>PMID:12194871</ref> <ref>PMID:12808128</ref> <ref>PMID:14523239</ref> <ref>PMID:12764195</ref> <ref>PMID:14630912</ref> <ref>PMID:15173182</ref> <ref>PMID:21076423</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many transient receptor potential (TRP) channels respond to diverse stimuli and conditionally conduct small and large cations. Such functional plasticity is presumably enabled by a uniquely dynamic ion selectivity filter that is regulated by physiological agents. What is currently missing is a "photo series" of intermediate structural states that directly address this hypothesis and reveal specific mechanisms behind such dynamic channel regulation. Here, we exploit cryoelectron microscopy (cryo-EM) to visualize conformational transitions of the capsaicin receptor, TRPV1, as a model to understand how dynamic transitions of the selectivity filter in response to algogenic agents, including protons, vanilloid agonists, and peptide toxins, permit permeation by small and large organic cations. These structures also reveal mechanisms governing ligand binding substates, as well as allosteric coupling between key sites that are proximal to the selectivity filter and cytoplasmic gate. These insights suggest a general framework for understanding how TRP channels function as polymodal signal integrators.
		+
		+	Structural snapshots of TRPV1 reveal mechanism of polymodal functionality.,Zhang K, Julius D, Cheng Y Cell. 2021 Aug 31. pii: S0092-8674(21)00982-X. doi: 10.1016/j.cell.2021.08.012. PMID:34496225<ref>PMID:34496225</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mz6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cheng Y]]	+	[[Category: Cheng, Y]]
-	[[Category: Julius D]]	+	[[Category: Julius, D]]
-	[[Category: Zhang K]]	+	[[Category: Zhang, K]]
		+	[[Category: Cryo-em]]
		+	[[Category: Nanodisc]]
		+	[[Category: Stoichiometry]]
		+	[[Category: Transport protein]]
		+	[[Category: Trp channel]]

OCA at 06:52, 22 September 2021

OCA — Wed, 22 Sep 2021 06:52:12 GMT

←Older revision		Revision as of 06:52, 22 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7mz6 ~~is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of minimal TRPV1 with 1 perturbed PI==
-		+	<StructureSection load='7mz6' size='340' side='right'caption='[[7mz6]]' scene=''>
-	~~Authors~~: ~~Zhang~~, K., ~~Julius~~, D., ~~Cheng~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZ6 FirstGlance]. <br>
-	~~Description: Cryo-EM structure of minimal TRPV1 with 1 perturbed PI~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mz6 OCA], [https://pdbe.org/7mz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7mz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mz6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cheng, Y]]	+	__TOC__
-	[[Category: ~~Zhang, K~~]]	+	</StructureSection>
-	[[Category: ~~Julius, D~~]]	+	[[Category: Large Structures]]
		+	[[Category: Cheng Y]]
		+	[[Category: Julius D]]
		+	[[Category: Zhang K]]

OCA at 05:07, 25 August 2021

OCA — Wed, 25 Aug 2021 05:07:30 GMT

←Older revision		Revision as of 05:07, 25 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mz6 is ON HOLD	+	The entry 7mz6 is ON HOLD until Paper Publication

	Authors: Zhang, K., Julius, D., Cheng, Y.		Authors: Zhang, K., Julius, D., Cheng, Y.

OCA at 09:24, 28 July 2021

OCA — Wed, 28 Jul 2021 09:24:44 GMT

←Older revision		Revision as of 09:24, 28 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mz6 is ON HOLD ~~until Paper Publication~~	+	The entry 7mz6 is ON HOLD

	Authors: Zhang, K., Julius, D., Cheng, Y.		Authors: Zhang, K., Julius, D., Cheng, Y.

OCA at 02:27, 2 July 2021

OCA — Fri, 02 Jul 2021 02:27:30 GMT

←Older revision		Revision as of 02:27, 2 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mz6 is ON HOLD	+	The entry 7mz6 is ON HOLD until Paper Publication

	Authors: Zhang, K., Julius, D., Cheng, Y.		Authors: Zhang, K., Julius, D., Cheng, Y.

OCA: Protected "7mz6" [edit=sysop:move=sysop]

OCA — Wed, 02 Jun 2021 13:30:39 GMT

Protected "7mz6" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:30, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7mz6 is ON HOLD Authors: Zhang, K., Julius, D., Cheng, Y. Description: Cryo-EM structure of minimal TRPV1 with 1 perturbed PI [[Category: Unrelease...

OCA — Wed, 02 Jun 2021 13:30:38 GMT

New page: '''Unreleased structure''' The entry 7mz6 is ON HOLD Authors: Zhang, K., Julius, D., Cheng, Y. Description: Cryo-EM structure of minimal TRPV1 with 1 perturbed PI [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 7mz6 is ON HOLD

Authors: Zhang, K., Julius, D., Cheng, Y.

Description: Cryo-EM structure of minimal TRPV1 with 1 perturbed PI
[[Category: Unreleased Structures]]
[[Category: Cheng, Y]]
[[Category: Zhang, K]]
[[Category: Julius, D]]