OCA at 16:22, 18 October 2023

OCA — Wed, 18 Oct 2023 16:22:17 GMT

←Older revision		Revision as of 16:22, 18 October 2023
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	==Structure of yeast pseudouridine synthase 7 (PUS7)==		==Structure of yeast pseudouridine synthase 7 (PUS7)==
-	<StructureSection load='7mzv' size='340' side='right'caption='[[7mzv]]' scene=''>	+	<StructureSection load='7mzv' size='340' side='right'caption='[[7mzv]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7mzv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mzv OCA], [https://pdbe.org/7mzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mzv RCSB], [https://www.ebi.ac.uk/pdbsum/7mzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mzv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mzv OCA], [https://pdbe.org/7mzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mzv RCSB], [https://www.ebi.ac.uk/pdbsum/7mzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mzv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PUS7_YEAST PUS7_YEAST] Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing (PubMed:12426583, PubMed:12682021, PubMed:15611063, PubMed:18435545, PubMed:19114708, PubMed:25219674). Also catalyzes pseudouridylation at position 56 in U2 snRNA (PubMed:21131909). Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr) (PubMed:14561887, PubMed:18332121, PubMed:25219674, PubMed:35058356). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA (PubMed:14561887). Also catalyzes pseudouridylation of mRNAs in response to heat shock: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAR-3' (PubMed:25219674, PubMed:35058356).<ref>PMID:12426583</ref> <ref>PMID:12682021</ref> <ref>PMID:14561887</ref> <ref>PMID:15611063</ref> <ref>PMID:18332121</ref> <ref>PMID:18435545</ref> <ref>PMID:19114708</ref> <ref>PMID:21131909</ref> <ref>PMID:25219674</ref> <ref>PMID:35058356</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudouridine (Psi) is a ubiquitous RNA modification incorporated by pseudouridine synthase (Pus) enzymes into hundreds of noncoding and protein-coding RNA substrates. Here, we determined the contributions of substrate structure and protein sequence to binding and catalysis by pseudouridine synthase 7 (Pus7), one of the principal messenger RNA (mRNA) modifying enzymes. Pus7 is distinct among the eukaryotic Pus proteins because it modifies a wider variety of substrates and shares limited homology with other Pus family members. We solved the crystal structure of Saccharomyces cerevisiae Pus7, detailing the architecture of the eukaryotic-specific insertions thought to be responsible for the expanded substrate scope of Pus7. Additionally, we identified an insertion domain in the protein that fine-tunes Pus7 activity both in vitro and in cells. These data demonstrate that Pus7 preferentially binds substrates possessing the previously identified UGUAR (R = purine) consensus sequence and that RNA secondary structure is not a strong requirement for Pus7-binding. In contrast, the rate constants and extent of Psi incorporation are more influenced by RNA structure, with Pus7 modifying UGUAR sequences in less-structured contexts more efficiently both in vitro and in cells. Although less-structured substrates were preferred, Pus7 fully modified every transfer RNA, mRNA, and nonnatural RNA containing the consensus recognition sequence that we tested. Our findings suggest that Pus7 is a promiscuous enzyme and lead us to propose that factors beyond inherent enzyme properties (e.g., enzyme localization, RNA structure, and competition with other RNA-binding proteins) largely dictate Pus7 substrate selection.
		+
		+	Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures.,Purchal MK, Eyler DE, Tardu M, Franco MK, Korn MM, Khan T, McNassor R, Giles R, Lev K, Sharma H, Monroe J, Mallik L, Koutmos M, Koutmou KS Proc Natl Acad Sci U S A. 2022 Jan 25;119(4). pii: 2109708119. doi:, 10.1073/pnas.2109708119. PMID:35058356<ref>PMID:35058356</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7mzv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pseudouridine synthase 3D structures\|Pseudouridine synthase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
	[[Category: Koutmos M]]		[[Category: Koutmos M]]
	[[Category: Purchal M]]		[[Category: Purchal M]]

OCA at 07:23, 9 February 2022

OCA — Wed, 09 Feb 2022 07:23:28 GMT

←Older revision		Revision as of 07:23, 9 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7mzv ~~is ON HOLD until Paper Publication~~	+	==Structure of yeast pseudouridine synthase 7 (PUS7)==
-		+	<StructureSection load='7mzv' size='340' side='right'caption='[[7mzv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MZV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mzv OCA], [https://pdbe.org/7mzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mzv RCSB], [https://www.ebi.ac.uk/pdbsum/7mzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mzv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Koutmos M]]
		+	[[Category: Purchal M]]

OCA at 11:06, 9 June 2021

OCA — Wed, 09 Jun 2021 11:06:23 GMT

←Older revision		Revision as of 11:06, 9 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7mzv is ON HOLD	+	The entry 7mzv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7mzv" [edit=sysop:move=sysop]

OCA — Wed, 02 Jun 2021 13:31:35 GMT

Protected "7mzv" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7mzv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Jun 2021 13:31:34 GMT

New page: '''Unreleased structure''' The entry 7mzv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7mzv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7mzv - Revision history

OCA at 16:22, 18 October 2023

OCA at 07:23, 9 February 2022

OCA at 11:06, 9 June 2021

OCA: Protected "7mzv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7mzv is ON HOLD Authors: Description: Category: Unreleased Structures