OCA at 16:22, 18 October 2023

OCA — Wed, 18 Oct 2023 16:22:37 GMT

←Older revision		Revision as of 16:22, 18 October 2023
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-	====	+	==Complex of recombinant Bet v 1 with Fab fragment of REGN5715==
-	<StructureSection load='7n0v' size='340' side='right'caption='[[7n0v]]' scene=''>	+	<StructureSection load='7n0v' size='340' side='right'caption='[[7n0v]], [[Resolution\|resolution]] 3.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7n0v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Betula_pendula Betula pendula] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N0V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n0v OCA], [https://pdbe.org/7n0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n0v RCSB], [https://www.ebi.ac.uk/pdbsum/7n0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n0v ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.71Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n0v OCA], [https://pdbe.org/7n0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n0v RCSB], [https://www.ebi.ac.uk/pdbsum/7n0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n0v ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN] May be a general steroid carrier protein (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Blocking the major cat allergen, Fel d 1, with mAbs was effective in preventing an acute cat allergic response. OBJECTIVES: This study sought to extend the allergen-specific antibody approach and demonstrate that a combination of mAbs targeting Bet v 1, the immunodominant and most abundant allergenic protein in birch pollen, can prevent the birch allergic response. METHODS: Bet v 1-specific mAbs, REGN5713, REGN5714, and REGN5715, were isolated using the VelocImmune platform. Surface plasmon resonance, x-ray crystallography, and cryo-electron microscopy determined binding kinetics and structural data. Inhibition of IgE-binding, basophil activation, and mast cell degranulation were assessed via blocking ELISA, flow cytometry, and the passive cutaneous anaphylaxis mouse model. RESULTS: REGN5713, REGN5714, and REGN5715 bind with high affinity and noncompetitively to Bet v 1. A cocktail of all 3 antibodies, REGN5713/14/15, blocks IgE binding to Bet v 1 and inhibits Bet v 1- and birch pollen extract-induced basophil activation ex vivo and mast cell degranulation in vivo. Crystal structures of the complex of Bet v 1 with immunoglobulin antigen-binding fragments of REGN5713 or REGN5715 show distinct interaction sites on Bet v 1. Cryo-electron microscopy reveals a planar and roughly symmetrical complex formed by REGN5713/14/15 bound to Bet v 1. CONCLUSIONS: These data confirm the immunodominance of Bet v 1 in birch allergy and demonstrate blockade of the birch allergic response with REGN5713/14/15. Structural analyses show simultaneous binding of REGN5713, REGN5714, and REGN5715 with substantial areas of Bet v 1 exposed, suggesting that targeting specific epitopes is sufficient to block the allergic response.
		+
		+	Targeting immunodominant Bet v 1 epitopes with monoclonal antibodies prevents the birch allergic response.,Atanasio A, Franklin MC, Kamat V, Hernandez AR, Badithe A, Ben LH, Jones J, Bautista J, Yancopoulos GD, Olson W, Murphy AJ, Sleeman MA, Orengo JM J Allergy Clin Immunol. 2022 Jan;149(1):200-211. doi: 10.1016/j.jaci.2021.05.038. , Epub 2021 Jun 11. PMID:34126155<ref>PMID:34126155</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7n0v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Betula pendula]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Franklin MC]]
		+	[[Category: Romero Hernandez A]]

OCA at 06:08, 11 August 2021

OCA — Wed, 11 Aug 2021 06:08:01 GMT

←Older revision		Revision as of 06:08, 11 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7n0v ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7n0v' size='340' side='right'caption='[[7n0v]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n0v OCA], [https://pdbe.org/7n0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n0v RCSB], [https://www.ebi.ac.uk/pdbsum/7n0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n0v ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 02:28, 2 July 2021

OCA — Fri, 02 Jul 2021 02:28:13 GMT

←Older revision		Revision as of 02:28, 2 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7n0v is ON HOLD	+	The entry 7n0v is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7n0v" [edit=sysop:move=sysop]

OCA — Wed, 02 Jun 2021 13:32:59 GMT

Protected "7n0v" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:32, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7n0v is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Jun 2021 13:32:58 GMT

New page: '''Unreleased structure''' The entry 7n0v is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7n0v is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7n0v - Revision history

OCA at 16:22, 18 October 2023

OCA at 06:08, 11 August 2021

OCA at 02:28, 2 July 2021

OCA: Protected "7n0v" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7n0v is ON HOLD Authors: Description: Category: Unreleased Structures