7n4t - Revision history

OCA at 19:41, 29 May 2024

OCA — Wed, 29 May 2024 19:41:48 GMT

←Older revision		Revision as of 19:41, 29 May 2024
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	==Low conductance mechanosensitive channel YnaI==		==Low conductance mechanosensitive channel YnaI==
-	<StructureSection load='7n4t' size='340' side='right'caption='[[7n4t]]' scene=''>	+	<StructureSection load='7n4t' size='340' side='right'caption='[[7n4t]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N4T FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N4T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n4t OCA], [https://pdbe.org/7n4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n4t RCSB], [https://www.ebi.ac.uk/pdbsum/7n4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n4t ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n4t OCA], [https://pdbe.org/7n4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n4t RCSB], [https://www.ebi.ac.uk/pdbsum/7n4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n4t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mechanosensitive channels respond to mechanical forces exerted on the cell membrane and play vital roles in regulating the chemical equilibrium within cells and their environment. High-resolution structural information is required to understand the gating mechanisms of mechanosensitive channels. Protein-lipid interactions are essential for the structural and functional integrity of mechanosensitive channels, but detergents cannot maintain the crucial native lipid environment for purified mechanosensitive channels. Recently, detergent-free systems have emerged as alternatives for membrane protein structural biology. This report shows that while membrane-active polymer, SMA2000, could retain some native cell membrane lipids on the transmembrane domain of the mechanosensitive-like YnaI channel, the complete structure of the transmembrane domain of YnaI was not resolved. This reveals a significant limitation of SMA2000 or similar membrane-active copolymers. This limitation may come from the heterogeneity of the polymers and nonspecific interactions between the polymers and the relatively large hydrophobic pockets within the transmembrane domain of YnaI. However, this limitation offers development opportunities for detergent-free technology for challenging membrane proteins.
		+
		+	Cryo-EM Structure of Mechanosensitive Channel YnaI Using SMA2000: Challenges and Opportunities.,Catalano C, Ben-Hail D, Qiu W, Blount P, des Georges A, Guo Y Membranes (Basel). 2021 Oct 31;11(11). pii: membranes11110849. doi:, 10.3390/membranes11110849. PMID:34832078<ref>PMID:34832078</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7n4t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 02:54, 21 April 2022

OCA — Thu, 21 Apr 2022 02:54:00 GMT

←Older revision		Revision as of 02:54, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7n4t is ~~ON HOLD until Paper Publication~~	+	==Low conductance mechanosensitive channel YnaI==
-		+	<StructureSection load='7n4t' size='340' side='right'caption='[[7n4t]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N4T FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n4t OCA], [https://pdbe.org/7n4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n4t RCSB], [https://www.ebi.ac.uk/pdbsum/7n4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n4t ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ben-Hail D]]
		+	[[Category: Catalano C]]
		+	[[Category: Guo Y]]
		+	[[Category: Qiu W]]
		+	[[Category: Des Georges A]]

OCA at 08:46, 7 July 2021

OCA — Wed, 07 Jul 2021 08:46:40 GMT

←Older revision		Revision as of 08:46, 7 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7n4t is ON HOLD	+	The entry 7n4t is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7n4t" [edit=sysop:move=sysop]

OCA — Thu, 17 Jun 2021 13:38:44 GMT

Protected "7n4t" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:38, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7n4t is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 17 Jun 2021 13:38:43 GMT

New page: '''Unreleased structure''' The entry 7n4t is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7n4t is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]