OCA at 16:24, 18 October 2023

OCA — Wed, 18 Oct 2023 16:24:42 GMT

←Older revision		Revision as of 16:24, 18 October 2023
Line 1:		Line 1:

-	====	+	==ZBTB7A Zinc Finger Domain Bound to -200 Site of Fetal Globin Promoter (Oligo 6)==
-	<StructureSection load='7n5s' size='340' side='right'caption='[[7n5s]]' scene=''>	+	<StructureSection load='7n5s' size='340' side='right'caption='[[7n5s]], [[Resolution\|resolution]] 2.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7n5s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5s OCA], [https://pdbe.org/7n5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5s RCSB], [https://www.ebi.ac.uk/pdbsum/7n5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.86Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5s OCA], [https://pdbe.org/7n5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5s RCSB], [https://www.ebi.ac.uk/pdbsum/7n5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ZBT7A_HUMAN ZBT7A_HUMAN] Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Elevated levels of fetal globin protect against beta-hemoglobinopathies, such as sickle cell disease and beta-thalassemia. Two zinc-finger (ZF) repressors, BCL11A and ZBTB7A/LRF, bind directly to the fetal globin promoter elements positioned at -115 and -200, respectively. Here, we describe X-ray structures of the ZBTB7A DNA-binding domain, consisting of four adjacent ZFs, in complex with the -200 sequence element, which contains two copies of four consecutive C:G base pairs. ZF1 and ZF2 recognize the 5' C:G quadruple, and ZF4 contacts the 3' C:G quadruple. Natural non-coding DNA mutations associated with hereditary persistence of fetal hemoglobin (HPFH) impair ZBTB7A DNA binding, with the most severe disruptions resulting from mutations in the base pairs recognized by ZF1 and ZF2. Our results firmly establish ZBTB7A/LRF as a key molecular regulator of fetal globin expression and inform genome-editing strategies that inhibit repressor binding and boost fetal globin expression to treat hemoglobinopathies.
		+
		+	Structural basis for human ZBTB7A action at the fetal globin promoter.,Yang Y, Ren R, Ly LC, Horton JR, Li F, Quinlan KGR, Crossley M, Shi Y, Cheng X Cell Rep. 2021 Sep 28;36(13):109759. doi: 10.1016/j.celrep.2021.109759. PMID:34592153<ref>PMID:34592153</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7n5s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Cheng X]]
		+	[[Category: Horton JR]]
		+	[[Category: Ren R]]

OCA at 06:19, 25 August 2021

OCA — Wed, 25 Aug 2021 06:19:59 GMT

←Older revision		Revision as of 06:19, 25 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7n5s ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7n5s' size='340' side='right'caption='[[7n5s]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5s OCA], [https://pdbe.org/7n5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5s RCSB], [https://www.ebi.ac.uk/pdbsum/7n5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5s ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7n5s" [edit=sysop:move=sysop]

OCA — Thu, 17 Jun 2021 13:40:01 GMT

Protected "7n5s" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:40, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7n5s is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 17 Jun 2021 13:40:00 GMT

New page: '''Unreleased structure''' The entry 7n5s is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7n5s is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7n5s - Revision history

OCA at 16:24, 18 October 2023

OCA at 06:19, 25 August 2021

OCA: Protected "7n5s" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7n5s is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures