OCA at 19:42, 29 May 2024

OCA — Wed, 29 May 2024 19:42:02 GMT

←Older revision		Revision as of 19:42, 29 May 2024
Line 1:		Line 1:

-	====	+	==ATP-bound TnsC-TniQ complex from ShCAST system==
-	<StructureSection load='7n6i' size='340' side='right'caption='[[7n6i]]' scene=''>	+	<StructureSection load='7n6i' size='340' side='right'caption='[[7n6i]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7n6i]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N6I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6i OCA], [https://pdbe.org/7n6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6i RCSB], [https://www.ebi.ac.uk/pdbsum/7n6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6i OCA], [https://pdbe.org/7n6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6i RCSB], [https://www.ebi.ac.uk/pdbsum/7n6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6i ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CRISPR-associated transposition systems allow guide RNA-directed integration of a single DNA cargo in one orientation at a fixed distance from a programmable target sequence. We used cryo-electron microscopy (cryo-EM) to define the mechanism that underlies this process by characterizing the transposition regulator, TnsC, from a type V-K CRISPR-transposase system. In this scenario, polymerization of adenosine triphosphate-bound TnsC helical filaments could explain how polarity information is passed to the transposase. TniQ caps the TnsC filament, representing a universal mechanism for target information transfer in Tn7/Tn7-like elements. Transposase-driven disassembly establishes delivery of the element only to unused protospacers. Finally, TnsC transitions to define the fixed point of insertion, as revealed by structures with the transition state mimic ADP*AlF(3) These mechanistic findings provide the underpinnings for engineering CRISPR-associated transposition systems for research and therapeutic applications.
		+
		+	Structural basis for target site selection in RNA-guided DNA transposition systems.,Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH Science. 2021 Aug 13;373(6556):768-774. doi: 10.1126/science.abi8976. Epub 2021 , Jul 15. PMID:34385391<ref>PMID:34385391</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7n6i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transposase 3D structures\|Transposase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Scytonema hofmannii]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Kellogg EH]]
		+	[[Category: Mehrotra E]]
		+	[[Category: Park J]]
		+	[[Category: Tsai AWL]]

OCA at 10:35, 28 July 2021

OCA — Wed, 28 Jul 2021 10:35:35 GMT

←Older revision		Revision as of 10:35, 28 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7n6i ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7n6i' size='340' side='right'caption='[[7n6i]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6i OCA], [https://pdbe.org/7n6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6i RCSB], [https://www.ebi.ac.uk/pdbsum/7n6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6i ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7n6i" [edit=sysop:move=sysop]

OCA — Thu, 17 Jun 2021 13:41:23 GMT

Protected "7n6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:41, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7n6i is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 17 Jun 2021 13:41:22 GMT

New page: '''Unreleased structure''' The entry 7n6i is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7n6i is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7n6i - Revision history

OCA at 19:42, 29 May 2024

OCA at 10:35, 28 July 2021

OCA: Protected "7n6i" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7n6i is ON HOLD Authors: Description: Category: Unreleased Structures