7nbi - Revision history

OCA at 12:32, 1 February 2024

OCA — Thu, 01 Feb 2024 12:32:17 GMT

←Older revision		Revision as of 12:32, 1 February 2024
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	==Crystal structure of a monomeric FLT3 Ligand variant==		==Crystal structure of a monomeric FLT3 Ligand variant==
-	<StructureSection load='7nbi' size='340' side='right'caption='[[7nbi]]' scene=''>	+	<StructureSection load='7nbi' size='340' side='right'caption='[[7nbi]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NBI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nbi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NBI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nbi OCA], [https://pdbe.org/7nbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nbi RCSB], [https://www.ebi.ac.uk/pdbsum/7nbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nbi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nbi OCA], [https://pdbe.org/7nbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nbi RCSB], [https://www.ebi.ac.uk/pdbsum/7nbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nbi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FLT3L_HUMAN FLT3L_HUMAN] Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The overarching paradigm for the activation of class III and V receptor tyrosine kinases (RTKs) prescribes cytokine-mediated dimerization of the receptor ectodomains and homotypic receptor-receptor interactions. However, structural studies have shown that the hematopoietic receptor FLT3, a class III RTK, does not appear to engage in such receptor-receptor contacts, despite its efficient dimerization by dimeric FLT3 ligand (FL). As part of efforts to better understand the intricacies of FLT3 activation, we sought to engineer a monomeric FL. It was found that a Leu27Asp substitution at the dimer interface of the cytokine led to a stable monomeric cytokine (FLL27D) without abrogation of receptor binding. The crystal structure of FLL27D at 1.65 A resolution revealed that the introduced point mutation led to shielding of the hydrophobic footprint of the dimerization interface in wild-type FL without affecting the conformation of the FLT3 binding site. Thus, FLL27D can serve as a monomeric FL variant to further interrogate the assembly mechanism of extracellular complexes of FLT3 in physiology and disease.
		+
		+	Engineering and crystal structure of a monomeric FLT3 ligand variant.,Pannecoucke E, Raes L, Savvides SN Acta Crystallogr F Struct Biol Commun. 2021 Apr 1;77(Pt 4):121-127. doi:, 10.1107/S2053230X21003289. Epub 2021 Apr 6. PMID:33830077<ref>PMID:33830077</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nbi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Pannecoucke E]]		[[Category: Pannecoucke E]]
	[[Category: Raes L]]		[[Category: Raes L]]
	[[Category: Savvides SN]]		[[Category: Savvides SN]]

OCA at 05:22, 28 April 2021

OCA — Wed, 28 Apr 2021 05:22:23 GMT

←Older revision		Revision as of 05:22, 28 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nbi is ~~ON HOLD~~	+	==Crystal structure of a monomeric FLT3 Ligand variant==
-		+	<StructureSection load='7nbi' size='340' side='right'caption='[[7nbi]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NBI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nbi OCA], [https://pdbe.org/7nbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nbi RCSB], [https://www.ebi.ac.uk/pdbsum/7nbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nbi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pannecoucke E]]
		+	[[Category: Raes L]]
		+	[[Category: Savvides SN]]

OCA at 09:26, 31 March 2021

OCA — Wed, 31 Mar 2021 09:26:59 GMT

←Older revision		Revision as of 09:26, 31 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nbi is ON HOLD ~~until Paper Publication~~	+	The entry 7nbi is ON HOLD

	Authors:		Authors:

OCA at 13:30, 3 March 2021

OCA — Wed, 03 Mar 2021 13:30:51 GMT

←Older revision		Revision as of 13:30, 3 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nbi is ON HOLD	+	The entry 7nbi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7nbi" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2021 09:35:16 GMT

Protected "7nbi" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:35, 3 February 2021

OCA: New page: '''Unreleased structure''' The entry 7nbi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Feb 2021 09:35:15 GMT

New page: '''Unreleased structure''' The entry 7nbi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7nbi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]