7nc6 - Revision history

OCA at 12:32, 1 February 2024

OCA — Thu, 01 Feb 2024 12:32:37 GMT

←Older revision		Revision as of 12:32, 1 February 2024
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	<StructureSection load='7nc6' size='340' side='right'caption='[[7nc6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='7nc6' size='340' side='right'caption='[[7nc6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7nc6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aspfc Aspfc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NC6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nc6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_A1163 Aspergillus fumigatus A1163]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NC6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=U8B:(2~{S})-2-azanyl-5-[[(2~{R})-3-[(2~{R},5~{R})-5-(hydroxymethyl)-3,6-bis(oxidanylidene)-2-(phenylmethyl)-5-sulfanyl-piperazin-2-yl]sulfanyl-1-(2-hydroxy-2-oxoethylamino)-1-oxidanylidene-propan-2-yl]amino]-5-oxidanylidene-pentanoic+acid'>U8B</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7nc5\|7nc5]], [[7nc3\|7nc3]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=U8B:(2~{S})-2-azanyl-5-[[(2~{R})-3-[(2~{R},5~{R})-5-(hydroxymethyl)-3,6-bis(oxidanylidene)-2-(phenylmethyl)-5-sulfanyl-piperazin-2-yl]sulfanyl-1-(2-hydroxy-2-oxoethylamino)-1-oxidanylidene-propan-2-yl]amino]-5-oxidanylidene-pentanoic+acid'>U8B</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AFUB_075740 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451804 ASPFC])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nc6 OCA], [https://pdbe.org/7nc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nc6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nc6 OCA], [https://pdbe.org/7nc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nc6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B0Y813_ASPFC B0Y813_ASPFC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7nc6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7nc6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspfc~~]]	+	[[Category: Aspergillus fumigatus A1163]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Groll, M]]	+	[[Category: Groll M]]
-	[[Category: Huber~~, E M]]~~	+	[[Category: Huber EM]]
-	~~[[Category: Aspergillus fumigatus]]~~	+
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Carbon-sulphur-bond]]~~	+
-	~~[[Category: Epidithiodioxopiperazine]]~~	+
-	~~[[Category: Glutathione-s-transferase]]~~	+
-	~~[[Category: Mycotoxin~~]]	+

OCA at 15:00, 17 June 2021

OCA — Thu, 17 Jun 2021 15:00:59 GMT

←Older revision		Revision as of 15:00, 17 June 2021
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	==Glutathione-S-transferase GliG in complex with cyclo[L-Phe-L-Ser]-bis-glutathione-adduct==		==Glutathione-S-transferase GliG in complex with cyclo[L-Phe-L-Ser]-bis-glutathione-adduct==
-	<StructureSection load='7nc6' size='340' side='right'caption='[[7nc6]]' scene=''>	+	<StructureSection load='7nc6' size='340' side='right'caption='[[7nc6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NC6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nc6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspfc Aspfc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NC6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nc6 OCA], [https://pdbe.org/7nc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nc6 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=U8B:(2~{S})-2-azanyl-5-[[(2~{R})-3-[(2~{R},5~{R})-5-(hydroxymethyl)-3,6-bis(oxidanylidene)-2-(phenylmethyl)-5-sulfanyl-piperazin-2-yl]sulfanyl-1-(2-hydroxy-2-oxoethylamino)-1-oxidanylidene-propan-2-yl]amino]-5-oxidanylidene-pentanoic+acid'>U8B</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7nc5\|7nc5]], [[7nc3\|7nc3]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AFUB_075740 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451804 ASPFC])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nc6 OCA], [https://pdbe.org/7nc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nc6 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutathione-S-transferases (GSTs) usually detoxify xenobiotics. The human pathogenic fungus Aspergillus fumigatus however uses the exceptional GST GliG to incorporate two sulphur atoms into its virulence factor gliotoxin. Because these sulphurs are essential for biological activity, glutathionylation is a key step of gliotoxin biosynthesis. Yet, the mechanism of carbon-sulphur linkage formation from a bis-hydroxylated precursor is unresolved. Here, we report structures of GliG with glutathione (GSH) and its reaction product cyclo[-l-Phe-l-Ser]-bis-glutathione, which has been purified from a genetically modified A. fumigatus strain. The structures argue for stepwise processing of first the Phe and second the Ser moiety. Enzyme-mediated dehydration of the substrate activates GSH and a helix dipole stabilizes the resulting anion via a water molecule for the nucleophilic attack. Activity assays with mutants validate the interactions of GliG with the ligands and enrich our knowledge about enzymatic C-S bond formation in gliotoxin and epipolythiodioxopiperazine (ETP) natural compounds in general.
		+
		+	Structural and mechanistic insights into C-S bond formation in gliotoxin.,Scherlach K, Kuttenlochner W, Scharf DH, Brakhage AA, Hertweck C, Groll M, Huber E Angew Chem Int Ed Engl. 2021 Apr 28. doi: 10.1002/anie.202104372. PMID:33909314<ref>PMID:33909314</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nc6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspfc]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Groll M]]	+	[[Category: Groll, M]]
-	[[Category: Huber EM]]	+	[[Category: Huber, E M]]
		+	[[Category: Aspergillus fumigatus]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Carbon-sulphur-bond]]
		+	[[Category: Epidithiodioxopiperazine]]
		+	[[Category: Glutathione-s-transferase]]
		+	[[Category: Mycotoxin]]

OCA at 09:23, 12 May 2021

OCA — Wed, 12 May 2021 09:23:13 GMT

←Older revision		Revision as of 09:23, 12 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nc6 ~~is ON HOLD until Paper Publication~~	+	==Glutathione-S-transferase GliG in complex with cyclo[L-Phe-L-Ser]-bis-glutathione-adduct==
-		+	<StructureSection load='7nc6' size='340' side='right'caption='[[7nc6]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NC6 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nc6 OCA], [https://pdbe.org/7nc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nc6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nc6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Groll M]]
		+	[[Category: Huber EM]]

OCA at 08:51, 17 February 2021

OCA — Wed, 17 Feb 2021 08:51:32 GMT

←Older revision		Revision as of 08:51, 17 February 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nc6 is ON HOLD	+	The entry 7nc6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7nc6" [edit=sysop:move=sysop]

OCA — Wed, 03 Feb 2021 09:36:55 GMT

Protected "7nc6" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:36, 3 February 2021

OCA: New page: '''Unreleased structure''' The entry 7nc6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Feb 2021 09:36:53 GMT

New page: '''Unreleased structure''' The entry 7nc6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7nc6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]