OCA at 12:34, 1 February 2024

OCA — Thu, 01 Feb 2024 12:34:23 GMT

←Older revision		Revision as of 12:34, 1 February 2024
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-	====	+	==Fujian capmidlink domain in complex with Nb8194==
-	<StructureSection load='7nfr' size='340' side='right'caption='[[7nfr]]' scene=''>	+	<StructureSection load='7nfr' size='340' side='right'caption='[[7nfr]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nfr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/duck/Fujian/13/2002(H5N1)) Influenza A virus (A/duck/Fujian/13/2002(H5N1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NFR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nfr OCA], [https://pdbe.org/7nfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nfr RCSB], [https://www.ebi.ac.uk/pdbsum/7nfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nfr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nfr OCA], [https://pdbe.org/7nfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nfr RCSB], [https://www.ebi.ac.uk/pdbsum/7nfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nfr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6E3N3_9INFA Q6E3N3_9INFA] Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex.[HAMAP-Rule:MF_04062][SAAS:SAAS01041023]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza A viruses cause seasonal epidemics and global pandemics, representing a considerable burden to healthcare systems. Central to the replication cycle of influenza viruses is the viral RNA-dependent RNA polymerase which transcribes and replicates the viral RNA genome. The polymerase undergoes conformational rearrangements and interacts with viral and host proteins to perform these functions. Here we determine the structure of the 1918 influenza virus polymerase in transcriptase and replicase conformations using cryo-electron microscopy (cryo-EM). We then structurally and functionally characterise the binding of single-domain nanobodies to the polymerase of the 1918 pandemic influenza virus. Combining these functional and structural data we identify five sites on the polymerase which are sensitive to inhibition by nanobodies. We propose that the binding of nanobodies at these sites either prevents the polymerase from assuming particular functional conformations or interactions with viral or host factors. The polymerase is highly conserved across the influenza A subtypes, suggesting these sites as effective targets for potential influenza antiviral development.
		+
		+	Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.,Keown JR, Zhu Z, Carrique L, Fan H, Walker AP, Serna Martin I, Pardon E, Steyaert J, Fodor E, Grimes JM Nat Commun. 2022 Jan 11;13(1):251. doi: 10.1038/s41467-021-27950-w. PMID:35017564<ref>PMID:35017564</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nfr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Fodor E]]
		+	[[Category: Grimes JM]]
		+	[[Category: Keown JR]]

OCA at 06:20, 1 December 2021

OCA — Wed, 01 Dec 2021 06:20:14 GMT

←Older revision		Revision as of 06:20, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nfr ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7nfr' size='340' side='right'caption='[[7nfr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nfr OCA], [https://pdbe.org/7nfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nfr RCSB], [https://www.ebi.ac.uk/pdbsum/7nfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nfr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7nfr" [edit=sysop:move=sysop]

OCA — Wed, 17 Feb 2021 08:54:05 GMT

Protected "7nfr" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7nfr is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Feb 2021 08:54:04 GMT

New page: '''Unreleased structure''' The entry 7nfr is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7nfr is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7nfr - Revision history

OCA at 12:34, 1 February 2024

OCA at 06:20, 1 December 2021

OCA: Protected "7nfr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7nfr is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures