7ng0 - Revision history

OCA at 12:34, 1 February 2024

OCA — Thu, 01 Feb 2024 12:34:29 GMT

←Older revision		Revision as of 12:34, 1 February 2024
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	<StructureSection load='7ng0' size='340' side='right'caption='[[7ng0]], [[Resolution\|resolution]] 2.95Å' scene=''>		<StructureSection load='7ng0' size='340' side='right'caption='[[7ng0]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ng0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Geobacillus_thermoleovorans_ccb_us3_uf5~~ Geobacillus thermoleovorans ~~ccb_us3_uf5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ng0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermoleovorans_CCB_US3_UF5 Geobacillus thermoleovorans CCB_US3_UF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.95Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~noc, GTCCBUS3UF5_39100 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111068 Geobacillus thermoleovorans CCB_US3_UF5])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng0 OCA], [https://pdbe.org/7ng0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng0 OCA], [https://pdbe.org/7ng0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/G8N1K9_GEOTH G8N1K9_GEOTH]] Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage.[HAMAP-Rule:MF_02015]	+	[https://www.uniprot.org/uniprot/G8N1K9_GEOTH G8N1K9_GEOTH] Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage.[HAMAP-Rule:MF_02015]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Geobacillus thermoleovorans ~~ccb_us3_uf5~~]]	+	[[Category: Geobacillus thermoleovorans CCB_US3_UF5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Errington, J]]	+	[[Category: Errington J]]
-	[[Category: Jalal~~, A S.B~~]]	+	[[Category: Jalal ASB]]
-	[[Category: Lawson~~, D M~~]]	+	[[Category: Lawson DM]]
-	[[Category: Le~~, T B.K~~]]	+	[[Category: Le TBK]]
-	[[Category: Ramakrishnan, K]]	+	[[Category: Ramakrishnan K]]
-	[[Category: Rejzek, M]]	+	[[Category: Rejzek M]]
-	[[Category: Stevenson~~, C E.M~~]]	+	[[Category: Stevenson CEM]]
-	[[Category: Tran~~, N T~~]]	+	[[Category: Tran NT]]
-	[[Category: Wu~~, L J]]~~	+	[[Category: Wu LJ]]
-	~~[[Category: Chromosome segregation]]~~	+
-	~~[[Category: Ctp switch]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Peripheral membrane protein]]~~	+
-	~~[[Category: Protein-dna recognition~~]]	+

OCA at 10:57, 28 July 2021

OCA — Wed, 28 Jul 2021 10:57:50 GMT

←Older revision		Revision as of 10:57, 28 July 2021
Line 1:		Line 1:

	==Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc==		==Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc==
-	<StructureSection load='7ng0' size='340' side='right'caption='[[7ng0]]' scene=''>	+	<StructureSection load='7ng0' size='340' side='right'caption='[[7ng0]], [[Resolution\|resolution]] 2.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ng0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermoleovorans_ccb_us3_uf5 Geobacillus thermoleovorans ccb_us3_uf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng0 OCA], [https://pdbe.org/7ng0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">noc, GTCCBUS3UF5_39100 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111068 Geobacillus thermoleovorans CCB_US3_UF5])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng0 OCA], [https://pdbe.org/7ng0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/G8N1K9_GEOTH G8N1K9_GEOTH]] Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage.[HAMAP-Rule:MF_02015]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ATP- and GTP-dependent molecular switches are extensively used to control functions of proteins in a wide range of biological processes. However, CTP switches are rarely reported. Here, we report that a nucleoid occlusion protein Noc is a CTPase enzyme whose membrane-binding activity is directly regulated by a CTP switch. In Bacillus subtilis, Noc nucleates on 16 bp NBS sites before associating with neighboring non-specific DNA to form large membrane-associated nucleoprotein complexes to physically occlude assembly of the cell division machinery. By in vitro reconstitution, we show that (1) CTP is required for Noc to form the NBS-dependent nucleoprotein complex, and (2) CTP binding, but not hydrolysis, switches Noc to a membrane-active state. Overall, we suggest that CTP couples membrane-binding activity of Noc to nucleoprotein complex formation to ensure productive recruitment of DNA to the bacterial cell membrane for nucleoid occlusion activity.
		+
		+	CTP regulates membrane-binding activity of the nucleoid occlusion protein Noc.,Jalal ASB, Tran NT, Wu LJ, Ramakrishnan K, Rejzek M, Gobbato G, Stevenson CEM, Lawson DM, Errington J, Le TBK Mol Cell. 2021 Jul 9. pii: S1097-2765(21)00505-0. doi:, 10.1016/j.molcel.2021.06.025. PMID:34270916<ref>PMID:34270916</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ng0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Geobacillus thermoleovorans ccb_us3_uf5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Errington J]]	+	[[Category: Errington, J]]
-	[[Category: Jalal ~~ASB~~]]	+	[[Category: Jalal, A S.B]]
-	[[Category: Lawson DM]]	+	[[Category: Lawson, D M]]
-	[[Category: Le ~~TBK~~]]	+	[[Category: Le, T B.K]]
-	[[Category: Ramakrishnan K]]	+	[[Category: Ramakrishnan, K]]
-	[[Category: Rejzek M]]	+	[[Category: Rejzek, M]]
-	[[Category: Stevenson ~~CEM~~]]	+	[[Category: Stevenson, C E.M]]
-	[[Category: Tran NT]]	+	[[Category: Tran, N T]]
-	[[Category: Wu LJ]]	+	[[Category: Wu, L J]]
		+	[[Category: Chromosome segregation]]
		+	[[Category: Ctp switch]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Peripheral membrane protein]]
		+	[[Category: Protein-dna recognition]]

OCA: Protected "7ng0" [edit=sysop:move=sysop]

OCA — Wed, 17 Feb 2021 10:03:14 GMT

Protected "7ng0" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:03, 17 February 2021

OCA: New page: ==Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc==
OCA — Wed, 17 Feb 2021 10:03:13 GMT

New page: ==Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc== <StructureSection load='7ng0' size='340' side='right'caption='7ng0' s...

New page

==Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc==
<StructureSection load='7ng0' size='340' side='right'caption='[[7ng0]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NG0 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ng0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ng0 OCA], [https://pdbe.org/7ng0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ng0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ng0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ng0 ProSAT]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Large Structures]]
[[Category: Errington J]]
[[Category: Jalal ASB]]
[[Category: Lawson DM]]
[[Category: Le TBK]]
[[Category: Ramakrishnan K]]
[[Category: Rejzek M]]
[[Category: Stevenson CEM]]
[[Category: Tran NT]]
[[Category: Wu LJ]]