7nje - Revision history

OCA at 12:35, 1 February 2024

OCA — Thu, 01 Feb 2024 12:35:32 GMT

←Older revision		Revision as of 12:35, 1 February 2024
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	==gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip==		==gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip==
-	<StructureSection load='7nje' size='340' side='right'caption='[[7nje]]' scene=''>	+	<StructureSection load='7nje' size='340' side='right'caption='[[7nje]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NJE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NJE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nje OCA], [https://pdbe.org/7nje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nje RCSB], [https://www.ebi.ac.uk/pdbsum/7nje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nje ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nje OCA], [https://pdbe.org/7nje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nje RCSB], [https://www.ebi.ac.uk/pdbsum/7nje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nje ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CRYGS_HUMAN CRYGS_HUMAN] Early-onset lamellar cataract;Early-onset sutural cataract. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CRYGS_HUMAN CRYGS_HUMAN] Crystallins are the dominant structural components of the vertebrate eye lens.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fixed-target serial crystallography has become an important method for the study of protein structure and dynamics at synchrotrons and X-ray free-electron lasers. However, sample homogeneity, consumption and the physical stress on samples remain major challenges for these high-throughput experiments, which depend on high-quality protein microcrystals. The batch crystallization procedures that are typically applied require time- and sample-intensive screening and optimization. Here, a simple protein crystallization method inside the features of the HARE serial crystallography chips is reported that circumvents batch crystallization and allows the direct transfer of canonical vapor-diffusion conditions to in-chip crystallization. Based on conventional hanging-drop vapor-diffusion experiments, the crystallization solution is distributed into the wells of the HARE chip and equilibrated against a reservoir with mother liquor. Using this simple method, high-quality microcrystals were generated with sufficient density for the structure determination of four different proteins. A new protein variant was crystallized using the protein concentrations encountered during canonical crystallization experiments, enabling structure determination from approximately 55 microg of protein. Additionally, structure determination from intracellular crystals grown in insect cells cultured directly in the features of the HARE chips is demonstrated. In cellulo crystallization represents a comparatively unexplored space in crystallization, especially for proteins that are resistant to crystallization using conventional techniques, and eliminates any need for laborious protein purification. This in-chip technique avoids harvesting the sensitive crystals or any further physical handling of the crystal-containing cells. These proof-of-principle experiments indicate the potential of this method to become a simple alternative to batch crystallization approaches and also as a convenient extension to canonical crystallization screens.
		+
		+	A simple vapor-diffusion method enables protein crystallization inside the HARE serial crystallography chip.,Norton-Baker B, Mehrabi P, Boger J, Schonherr R, von Stetten D, Schikora H, Kwok AO, Martin RW, Miller RJD, Redecke L, Schulz EC Acta Crystallogr D Struct Biol. 2021 Jun 1;77(Pt 6):820-834. doi:, 10.1107/S2059798321003855. Epub 2021 May 19. PMID:34076595<ref>PMID:34076595</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nje" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Crystallin 3D structures\|Crystallin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Boger J]]		[[Category: Boger J]]

OCA at 14:47, 17 June 2021

OCA — Thu, 17 Jun 2021 14:47:51 GMT

←Older revision		Revision as of 14:47, 17 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nje ~~is ON HOLD until Paper Publication~~	+	==gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip==
-		+	<StructureSection load='7nje' size='340' side='right'caption='[[7nje]]' scene=''>
-	~~Authors~~: ~~Norton~~-~~Baker, B~~.~~, Mehrabi, P~~.~~, Boger, J~~.~~, Schonherr, R~~.~~, von Stetten, D~~.~~, Schikora, H~~., ~~Martin, R~~.W., ~~Miller~~, R.J.D., ~~Redecke, L~~., ~~Schulz, E~~.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NJE FirstGlance]. <br>
-	~~Description: gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nje OCA], [https://pdbe.org/7nje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nje RCSB], [https://www.ebi.ac.uk/pdbsum/7nje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nje ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Miller, R.~~J.D]]	+	__TOC__
-	[[Category: ~~Mehrabi, P~~]]	+	</StructureSection>
-	[[Category: ~~Boger, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Redecke, L~~]]	+	[[Category: Boger J]]
-	[[Category: ~~Schonherr, R~~]]	+	[[Category: Martin RW]]
-	[[Category: ~~Von Stetten, D~~]]	+	[[Category: Mehrabi P]]
-	[[Category: ~~Norton-Baker, B~~]]	+	[[Category: Miller RJD]]
-	[[Category: ~~Martin,~~ R.W]]	+	[[Category: Norton-Baker B]]
-	[[Category: ~~Schikora, H~~]]	+	[[Category: Redecke L]]
-	[[Category: ~~Schulz, E.C~~]]	+	[[Category: Schikora H]]
		+	[[Category: Schonherr R]]
		+	[[Category: Schulz EC]]
		+	[[Category: Von Stetten D]]

OCA at 05:36, 24 March 2021

OCA — Wed, 24 Mar 2021 05:36:30 GMT

←Older revision		Revision as of 05:36, 24 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nje is ON HOLD	+	The entry 7nje is ON HOLD until Paper Publication

	Authors: Norton-Baker, B., Mehrabi, P., Boger, J., Schonherr, R., von Stetten, D., Schikora, H., Martin, R.W., Miller, R.J.D., Redecke, L., Schulz, E.C.		Authors: Norton-Baker, B., Mehrabi, P., Boger, J., Schonherr, R., von Stetten, D., Schikora, H., Martin, R.W., Miller, R.J.D., Redecke, L., Schulz, E.C.
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	Description: gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip		Description: gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Miller, R.J.D]]
	[[Category: Mehrabi, P]]		[[Category: Mehrabi, P]]
		+	[[Category: Boger, J]]
	[[Category: Redecke, L]]		[[Category: Redecke, L]]
	[[Category: Schonherr, R]]		[[Category: Schonherr, R]]
	[[Category: Von Stetten, D]]		[[Category: Von Stetten, D]]
-	[[Category: Martin, R.W]]
-	[[Category: Miller, R.J.D]]
-	[[Category: Boger, J]]
	[[Category: Norton-Baker, B]]		[[Category: Norton-Baker, B]]
		+	[[Category: Martin, R.W]]
	[[Category: Schikora, H]]		[[Category: Schikora, H]]
	[[Category: Schulz, E.C]]		[[Category: Schulz, E.C]]

OCA: Protected "7nje" [edit=sysop:move=sysop]

OCA — Wed, 24 Feb 2021 05:47:08 GMT

Protected "7nje" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:47, 24 February 2021

OCA: New page: '''Unreleased structure''' The entry 7nje is ON HOLD Authors: Norton-Baker, B., Mehrabi, P., Boger, J., Schonherr, R., von Stetten, D., Schikora, H., Martin, R.W., Miller, R.J.D., Redec...

OCA — Wed, 24 Feb 2021 05:47:07 GMT

New page: '''Unreleased structure''' The entry 7nje is ON HOLD Authors: Norton-Baker, B., Mehrabi, P., Boger, J., Schonherr, R., von Stetten, D., Schikora, H., Martin, R.W., Miller, R.J.D., Redec...

New page

'''Unreleased structure'''

The entry 7nje is ON HOLD

Authors: Norton-Baker, B., Mehrabi, P., Boger, J., Schonherr, R., von Stetten, D., Schikora, H., Martin, R.W., Miller, R.J.D., Redecke, L., Schulz, E.C.

Description: gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip
[[Category: Unreleased Structures]]
[[Category: Mehrabi, P]]
[[Category: Redecke, L]]
[[Category: Schonherr, R]]
[[Category: Von Stetten, D]]
[[Category: Martin, R.W]]
[[Category: Miller, R.J.D]]
[[Category: Boger, J]]
[[Category: Norton-Baker, B]]
[[Category: Schikora, H]]
[[Category: Schulz, E.C]]