OCA at 08:00, 10 November 2021

OCA — Wed, 10 Nov 2021 08:00:21 GMT

←Older revision		Revision as of 08:00, 10 November 2021
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	==cytoplasmic domain of Vibrio cholerae ToxR==		==cytoplasmic domain of Vibrio cholerae ToxR==
-	<StructureSection load='7nmb' size='340' side='right'caption='[[7nmb]]' scene=''>	+	<StructureSection load='7nmb' size='340' side='right'caption='[[7nmb]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NMB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nmb]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NMB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmb OCA], [https://pdbe.org/7nmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmb RCSB], [https://www.ebi.ac.uk/pdbsum/7nmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmb ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmb OCA], [https://pdbe.org/7nmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmb RCSB], [https://www.ebi.ac.uk/pdbsum/7nmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmb ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ToxR represents an essential transcription factor of Vibrio cholerae, which is involved in the regulation of multiple, mainly virulence associated genes. Its versatile functionality as activator, respressor or co-activator suggests a complex regulatory mechanism, whose clarification is essential for a better understanding of the virulence expression system of V. cholerae. Here, we provide structural information elucidating the organization and binding behaviour of the cytoplasmic DNA binding domain of ToxR (cToxR), containing a winged helix-turn-helix (wHTH) motif. Our analysis reveals unexpected structural features of this domain expanding our knowledge of a poorly-defined subfamily of wHTH proteins. cToxR forms an extraordinary long alpha-loop and furthermore has an additional C-terminal beta strand, contacting the N-terminus and thus leading to a compact fold. The identification of the exact interactions between ToxR and DNA contributes to a deeper understanding of this regulatory process. Our findings not only show general binding of the soluble cytoplasmic domain of ToxR to DNA, but also indicate a higher affinity for the toxT motif. These results support the current theory of ToxR being a 'DNA-catcher' to enable binding of the transcription factor TcpP and thus activation of virulence-associated toxT transcription. Although, TcpP and ToxR interaction is assumed to be crucial in the activation of the toxT genes, we could not detect an interaction event of their isolated cytoplasmic domains. We therefore conclude that other factors are needed to establish this protein-protein interaction, e.g., membrane attachment, the presence of their full-length proteins and/or other intermediary proteins that may facilitate binding.
		+
		+	Structural and DNA binding properties of the cytoplasmic domain of Vibrio cholerae transcription factor ToxR.,Gubensak N, Schrank E, Hartlmuller C, Gobl C, Falsone FS, Becker W, Wagner GE, Pulido S, Meyer NH, Pavkov-Keller T, Madl T, Reidl J, Zangger K J Biol Chem. 2021 Sep 3:101167. doi: 10.1016/j.jbc.2021.101167. PMID:34487759<ref>PMID:34487759</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nmb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gubensaek N]]	+	[[Category: Gubensaek, N]]
-	[[Category: Hartlmueller C]]	+	[[Category: Hartlmueller, C]]
-	[[Category: Madl T]]	+	[[Category: Madl, T]]
-	[[Category: Zangger K]]	+	[[Category: Zangger, K]]
		+	[[Category: Dna binding domain whth transcription factor]]
		+	[[Category: Transcription]]

OCA at 16:30, 20 October 2021

OCA — Wed, 20 Oct 2021 16:30:08 GMT

←Older revision		Revision as of 16:30, 20 October 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nmb is ~~ON HOLD until Paper Publication~~	+	==cytoplasmic domain of Vibrio cholerae ToxR==
-		+	<StructureSection load='7nmb' size='340' side='right'caption='[[7nmb]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NMB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmb OCA], [https://pdbe.org/7nmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmb RCSB], [https://www.ebi.ac.uk/pdbsum/7nmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmb ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gubensaek N]]
		+	[[Category: Hartlmueller C]]
		+	[[Category: Madl T]]
		+	[[Category: Zangger K]]

OCA at 05:32, 17 March 2021

OCA — Wed, 17 Mar 2021 05:32:12 GMT

←Older revision		Revision as of 05:32, 17 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nmb is ON HOLD	+	The entry 7nmb is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7nmb" [edit=sysop:move=sysop]

OCA — Wed, 03 Mar 2021 13:35:18 GMT

Protected "7nmb" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:35, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7nmb is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Mar 2021 13:35:16 GMT

New page: '''Unreleased structure''' The entry 7nmb is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7nmb is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7nmb - Revision history

OCA at 08:00, 10 November 2021

OCA at 16:30, 20 October 2021

OCA at 05:32, 17 March 2021

OCA: Protected "7nmb" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7nmb is ON HOLD Authors: Description: Category: Unreleased Structures