7nmz - Revision history

OCA at 12:37, 1 February 2024

OCA — Thu, 01 Feb 2024 12:37:07 GMT

←Older revision		Revision as of 12:37, 1 February 2024
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-	====	+	==Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-3-3 binding motifs Ser342 and Ser448==
-	<StructureSection load='7nmz' size='340' side='right'caption='[[7nmz]]' scene=''>	+	<StructureSection load='7nmz' size='340' side='right'caption='[[7nmz]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nmz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NMZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmz OCA], [https://pdbe.org/7nmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmz RCSB], [https://www.ebi.ac.uk/pdbsum/7nmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.303Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmz OCA], [https://pdbe.org/7nmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmz RCSB], [https://www.ebi.ac.uk/pdbsum/7nmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/1433F_HUMAN 1433F_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.<ref>PMID:12177059</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neural precursor cell expressed developmentally down-regulated 4 ligase (Nedd4-2) is an E3 ubiquitin ligase that targets proteins for ubiquitination and endocytosis, thereby regulating numerous ion channels, membrane receptors and tumor suppressors. Nedd4-2 activity is regulated by autoinhibition, calcium binding, oxidative stress, substrate binding, phosphorylation and 14-3-3 protein binding. However, the structural basis of 14-3-3-mediated Nedd4-2 regulation remains poorly understood. Here, we combined several techniques of integrative structural biology to characterize Nedd4-2 and its complex with 14-3-3. We demonstrate that phosphorylated Ser(342) and Ser(448) are the key residues that facilitate 14-3-3 protein binding to Nedd4-2 and that 14-3-3 protein binding induces a structural rearrangement of Nedd4-2 by inhibiting interactions between its structured domains. Overall, our findings provide the structural glimpse into the 14-3-3-mediated Nedd4-2 regulation and highlight the potential of the Nedd4-2:14-3-3 complex as a pharmacological target for Nedd4-2-associated diseases such as hypertension, epilepsy, kidney disease and cancer.
		+
		+	14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains.,Pohl P, Joshi R, Petrvalska O, Obsil T, Obsilova V Commun Biol. 2021 Jul 22;4(1):899. doi: 10.1038/s42003-021-02419-0. PMID:34294877<ref>PMID:34294877</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nmz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Obsil T]]
		+	[[Category: Obsilova V]]
		+	[[Category: Pohl P]]

OCA at 08:59, 21 July 2021

OCA — Wed, 21 Jul 2021 08:59:19 GMT

←Older revision		Revision as of 08:59, 21 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nmz ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7nmz' size='340' side='right'caption='[[7nmz]]' scene=''>
-	~~Authors~~: ~~Pohl, P~~.~~, Obsil, T~~.~~, Obsilova, V~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Structure of 14~~-~~3-3 eta in complex with Nedd4-2(335-455) containing two 14-3-3 binding motifs Ser342 and Ser448~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nmz OCA], [https://pdbe.org/7nmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nmz RCSB], [https://www.ebi.ac.uk/pdbsum/7nmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nmz ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Pohl, P~~]]	+	__TOC__
-	[[Category: ~~Obsil, T~~]]	+	</StructureSection>
-	[[Category: ~~Obsilova, V~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 14:57, 10 March 2021

OCA — Wed, 10 Mar 2021 14:57:59 GMT

←Older revision		Revision as of 14:57, 10 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nmz is ON HOLD	+	The entry 7nmz is ON HOLD until Paper Publication

	Authors: Pohl, P., Obsil, T., Obsilova, V.		Authors: Pohl, P., Obsil, T., Obsilova, V.

OCA: Protected "7nmz" [edit=sysop:move=sysop]

OCA — Wed, 03 Mar 2021 13:36:23 GMT

Protected "7nmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:36, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7nmz is ON HOLD Authors: Pohl, P., Obsil, T., Obsilova, V. Description: Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-...

OCA — Wed, 03 Mar 2021 13:36:22 GMT

New page: '''Unreleased structure''' The entry 7nmz is ON HOLD Authors: Pohl, P., Obsil, T., Obsilova, V. Description: Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-...

New page

'''Unreleased structure'''

The entry 7nmz is ON HOLD

Authors: Pohl, P., Obsil, T., Obsilova, V.

Description: Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-3-3 binding motifs Ser342 and Ser448
[[Category: Unreleased Structures]]
[[Category: Pohl, P]]
[[Category: Obsil, T]]
[[Category: Obsilova, V]]