7np8 - Revision history

OCA at 09:00, 6 December 2023

OCA — Wed, 06 Dec 2023 09:00:35 GMT

←Older revision		Revision as of 09:00, 6 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7np8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NP8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7np8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NP8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SRM:SIROHEME'>SRM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SRM:SIROHEME'>SRM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7np8 OCA], [https://pdbe.org/7np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7np8 RCSB], [https://www.ebi.ac.uk/pdbsum/7np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7np8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7np8 OCA], [https://pdbe.org/7np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7np8 RCSB], [https://www.ebi.ac.uk/pdbsum/7np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7np8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:36, 7 April 2023

OCA — Fri, 07 Apr 2023 06:36:33 GMT

←Older revision		Revision as of 06:36, 7 April 2023
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	==Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution==		==Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution==
-	<StructureSection load='7np8' size='340' side='right'caption='[[7np8]]' scene=''>	+	<StructureSection load='7np8' size='340' side='right'caption='[[7np8]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7np8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NP8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7np8 OCA], [https://pdbe.org/7np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7np8 RCSB], [https://www.ebi.ac.uk/pdbsum/7np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7np8 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SRM:SIROHEME'>SRM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7np8 OCA], [https://pdbe.org/7np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7np8 RCSB], [https://www.ebi.ac.uk/pdbsum/7np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7np8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FSR_METJA FSR_METJA] Catalyzes the reduction of sulfite to sulfide using reduced F420 as the electron source. Involved in sulfite detoxification and assimilation. Cannot use NADH or NADPH.<ref>PMID:16048999</ref> <ref>PMID:18378657</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methanogenic archaea are main actors in the carbon cycle but are sensitive to reactive sulfite. Some methanogens use a sulfite detoxification system that combines an F(420)H(2)-oxidase with a sulfite reductase, both of which are proposed precursors of modern enzymes. Here, we present snapshots of this coupled system, named coenzyme F(420)-dependent sulfite reductase (Group I Fsr), obtained from two marine methanogens. Fsr organizes as a homotetramer, harboring an intertwined six-[4Fe-4S] cluster relay characterized by spectroscopy. The wire, spanning 5.4 nm, electronically connects the flavin to the siroheme center. Despite a structural architecture similar to dissimilatory sulfite reductases, Fsr shows a siroheme coordination and a reaction mechanism identical to assimilatory sulfite reductases. Accordingly, the reaction of Fsr is unidirectional, reducing sulfite or nitrite with F(420)H(2). Our results provide structural insights into this unique fusion, in which a primitive sulfite reductase turns a poison into an elementary block of life.
		+
		+	Structures of the sulfite detoxifying F(420)-dependent enzyme from Methanococcales.,Jespersen M, Pierik AJ, Wagner T Nat Chem Biol. 2023 Jan 19. doi: 10.1038/s41589-022-01232-y. PMID:36658338<ref>PMID:36658338</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7np8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Methanocaldococcus jannaschii DSM 2661]]
	[[Category: Jespersen M]]		[[Category: Jespersen M]]
	[[Category: Wagner T]]		[[Category: Wagner T]]

OCA at 11:17, 23 March 2022

OCA — Wed, 23 Mar 2022 11:17:32 GMT

←Older revision		Revision as of 11:17, 23 March 2022
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-	'''Unreleased structure'''

-	~~The entry 7np8 is ON HOLD until Paper Publication~~	+	==Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution==
-		+	<StructureSection load='7np8' size='340' side='right'caption='[[7np8]]' scene=''>
-	~~Authors: Jespersen, M., Wagner, T.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NP8 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7np8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7np8 OCA], [https://pdbe.org/7np8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7np8 RCSB], [https://www.ebi.ac.uk/pdbsum/7np8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7np8 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Wagner~~, T]]	+	__TOC__
-	[[Category: Jespersen, M]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jespersen M]]
		+	[[Category: Wagner T]]

OCA at 09:29, 31 March 2021

OCA — Wed, 31 Mar 2021 09:29:14 GMT

←Older revision		Revision as of 09:29, 31 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7np8 is ON HOLD	+	The entry 7np8 is ON HOLD until Paper Publication

	Authors: Jespersen, M., Wagner, T.		Authors: Jespersen, M., Wagner, T.

OCA at 05:32, 17 March 2021

OCA — Wed, 17 Mar 2021 05:32:40 GMT

←Older revision		Revision as of 05:32, 17 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7np8 is ON HOLD ~~until sometime in the future~~	+	The entry 7np8 is ON HOLD

	Authors: Jespersen, M., Wagner, T.		Authors: Jespersen, M., Wagner, T.

OCA: Protected "7np8" [edit=sysop:move=sysop]

OCA — Wed, 10 Mar 2021 15:00:43 GMT

Protected "7np8" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 10 March 2021

OCA: New page: '''Unreleased structure''' The entry 7np8 is ON HOLD until sometime in the future Authors: Jespersen, M., Wagner, T. Description: Crystal structure of the Coenzyme F420-dependent sulfi...

OCA — Wed, 10 Mar 2021 15:00:41 GMT

New page: '''Unreleased structure''' The entry 7np8 is ON HOLD until sometime in the future Authors: Jespersen, M., Wagner, T. Description: Crystal structure of the Coenzyme F420-dependent sulfi...

New page

'''Unreleased structure'''

The entry 7np8 is ON HOLD until sometime in the future

Authors: Jespersen, M., Wagner, T.

Description: Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution
[[Category: Unreleased Structures]]
[[Category: Wagner, T]]
[[Category: Jespersen, M]]