7nqd - Revision history

OCA at 08:12, 25 May 2022

OCA — Wed, 25 May 2022 08:12:34 GMT

←Older revision		Revision as of 08:12, 25 May 2022
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-	==~~Archaeo~~-~~Eukaryotic Primase (AEP)~~ Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137==	+	==Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137==
	<StructureSection load='7nqd' size='340' side='right'caption='[[7nqd]], [[Resolution\|resolution]] 2.97Å' scene=''>		<StructureSection load='7nqd' size='340' side='right'caption='[[7nqd]], [[Resolution\|resolution]] 2.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 10:23, 18 May 2022

OCA — Wed, 18 May 2022 10:23:16 GMT

←Older revision		Revision as of 10:23, 18 May 2022
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	==Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137==		==Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137==
-	<StructureSection load='7nqd' size='340' side='right'caption='[[7nqd]]' scene=''>	+	<StructureSection load='7nqd' size='340' side='right'caption='[[7nqd]], [[Resolution\|resolution]] 2.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NQD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nqd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NQD FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqd OCA], [https://pdbe.org/7nqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqd RCSB], [https://www.ebi.ac.uk/pdbsum/7nqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqd ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqd OCA], [https://pdbe.org/7nqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqd RCSB], [https://www.ebi.ac.uk/pdbsum/7nqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqd ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During the initiation of DNA replication, oligonucleotide primers are synthesized de novo by primases and are subsequently extended by replicative polymerases to complete genome duplication. The primase-polymerase (Prim-Pol) superfamily is a diverse grouping of primases, which includes replicative primases and CRISPR-associated primase-polymerases (CAPPs) involved in adaptive immunity(1-3). Although much is known about the activities of these enzymes, the precise mechanism used by primases to initiate primer synthesis has not been elucidated. Here we identify the molecular bases for the initiation of primer synthesis by CAPP and show that this mechanism is also conserved in replicative primases. The crystal structure of a primer initiation complex reveals how the incoming nucleotides are positioned within the active site, adjacent to metal cofactors and paired to the templating single-stranded DNA strand, before synthesis of the first phosphodiester bond. Furthermore, the structure of a Prim-Pol complex with double-stranded DNA shows how the enzyme subsequently extends primers in a processive polymerase mode. The structural and mechanistic studies presented here establish how Prim-Pol proteins instigate primer synthesis, revealing the requisite molecular determinants for primer synthesis within the catalytic domain. This work also establishes that the catalytic domain of Prim-Pol enzymes, including replicative primases, is sufficient to catalyse primer formation.
		+
		+	Molecular basis for the initiation of DNA primer synthesis.,Li AWH, Zabrady K, Bainbridge LJ, Zabrady M, Naseem-Khan S, Berger MB, Kolesar P, Cisneros GA, Doherty AJ Nature. 2022 May 4. pii: 10.1038/s41586-022-04695-0. doi:, 10.1038/s41586-022-04695-0. PMID:35508653<ref>PMID:35508653</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nqd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Doherty AJ]]	+	[[Category: Doherty, A J]]
-	[[Category: Li ~~AWH~~]]	+	[[Category: Li, A W.H]]
		+	[[Category: Aep archaeo-eukaryotic primase apo prim-pol primase-polymerase primase polymerase]]
		+	[[Category: Transferase]]

OCA at 12:47, 4 May 2022

OCA — Wed, 04 May 2022 12:47:21 GMT

←Older revision		Revision as of 12:47, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7nqd ~~is ON HOLD until Paper Publication~~	+	==Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137==
-		+	<StructureSection load='7nqd' size='340' side='right'caption='[[7nqd]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NQD FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nqd OCA], [https://pdbe.org/7nqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nqd RCSB], [https://www.ebi.ac.uk/pdbsum/7nqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nqd ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Doherty AJ]]
		+	[[Category: Li AWH]]

OCA at 09:29, 31 March 2021

OCA — Wed, 31 Mar 2021 09:29:55 GMT

←Older revision		Revision as of 09:29, 31 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nqd is ON HOLD	+	The entry 7nqd is ON HOLD until Paper Publication

-	Authors: ~~Li, A.W.H., Doherty, A.J.~~	+	Authors:

-	Description: ~~Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Doherty, A.J]]
-	[[Category: Li, A.W.H]]

OCA: Protected "7nqd" [edit=sysop:move=sysop]

OCA — Wed, 10 Mar 2021 15:03:49 GMT

Protected "7nqd" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:03, 10 March 2021

OCA: New page: '''Unreleased structure''' The entry 7nqd is ON HOLD Authors: Li, A.W.H., Doherty, A.J. Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from M...

OCA — Wed, 10 Mar 2021 15:03:47 GMT

New page: '''Unreleased structure''' The entry 7nqd is ON HOLD Authors: Li, A.W.H., Doherty, A.J. Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from M...

New page

'''Unreleased structure'''

The entry 7nqd is ON HOLD

Authors: Li, A.W.H., Doherty, A.J.

Description: Archaeo-Eukaryotic Primase (AEP) Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137
[[Category: Unreleased Structures]]
[[Category: Doherty, A.J]]
[[Category: Li, A.W.H]]