OCA at 12:39, 1 February 2024

OCA — Thu, 01 Feb 2024 12:39:35 GMT

←Older revision		Revision as of 12:39, 1 February 2024
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	==Human myelin P2 mutant V115A==		==Human myelin P2 mutant V115A==
-	<StructureSection load='7ntp' size='340' side='right'caption='[[7ntp]]' scene=''>	+	<StructureSection load='7ntp' size='340' side='right'caption='[[7ntp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ntp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NTP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ntp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ntp OCA], [https://pdbe.org/7ntp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ntp RCSB], [https://www.ebi.ac.uk/pdbsum/7ntp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ntp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ntp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ntp OCA], [https://pdbe.org/7ntp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ntp RCSB], [https://www.ebi.ac.uk/pdbsum/7ntp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ntp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MYP2_HUMAN MYP2_HUMAN] May play a role in lipid transport protein in Schwann cells. May bind cholesterol.<ref>PMID:20421974</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peripheral myelin protein 2 (P2) is a fatty acid-binding protein expressed in vertebrate peripheral nervous system myelin, as well as in human astrocytes. Suggested functions of P2 include membrane stacking and lipid transport. Mutations in the PMP2 gene, encoding P2, are associated with Charcot-Marie-Tooth disease (CMT). Recent studies have revealed three novel PMP2 mutations in CMT patients. To shed light on the structure and function of these P2 variants, we used X-ray and neutron crystallography, small-angle X-ray scattering, circular dichroism spectroscopy, computer simulations and lipid binding assays. The crystal and solution structures of the I50del, M114T and V115A variants of P2 showed minor differences to the wild-type protein, whereas their thermal stability was reduced. Vesicle aggregation assays revealed no change in membrane stacking characteristics, while the variants showed altered fatty acid binding. Time-lapse imaging of lipid bilayers indicated formation of double-membrane structures induced by P2, which could be related to its function in stacking of two myelin membrane surfaces in vivo. In order to better understand the links between structure, dynamics and function, the crystal structure of perdeuterated P2 was refined from room temperature data using neutrons and X-rays, and the results were compared to simulations and cryocooled crystal structures. Our data indicate similar properties for all known human P2 CMT variants; while crystal structures are nearly identical, thermal stability and function of CMT variants are impaired. Our data provide new insights into the structure-function relationships and dynamics of P2 in health and disease.
		+
		+	Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants.,Uusitalo M, Klenow MB, Laulumaa S, Blakeley MP, Simonsen AC, Ruskamo S, Kursula P FEBS J. 2021 Jun 17. doi: 10.1111/febs.16079. PMID:34138518<ref>PMID:34138518</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ntp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kursula P]]		[[Category: Kursula P]]
	[[Category: Ruskamo S]]		[[Category: Ruskamo S]]
	[[Category: Uusitalo M]]		[[Category: Uusitalo M]]

OCA at 16:59, 15 December 2021

OCA — Wed, 15 Dec 2021 16:59:29 GMT

←Older revision		Revision as of 16:59, 15 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7ntp is ~~ON HOLD until Paper Publication~~	+	==Human myelin P2 mutant V115A==
-		+	<StructureSection load='7ntp' size='340' side='right'caption='[[7ntp]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NTP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ntp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ntp OCA], [https://pdbe.org/7ntp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ntp RCSB], [https://www.ebi.ac.uk/pdbsum/7ntp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ntp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kursula P]]
		+	[[Category: Ruskamo S]]
		+	[[Category: Uusitalo M]]

OCA at 04:45, 7 April 2021

OCA — Wed, 07 Apr 2021 04:45:53 GMT

←Older revision		Revision as of 04:45, 7 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ntp is ON HOLD	+	The entry 7ntp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ntp" [edit=sysop:move=sysop]

OCA — Wed, 17 Mar 2021 05:36:41 GMT

Protected "7ntp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 17 March 2021

OCA: New page: '''Unreleased structure''' The entry 7ntp is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Mar 2021 05:36:40 GMT

New page: '''Unreleased structure''' The entry 7ntp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ntp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ntp - Revision history

OCA at 12:39, 1 February 2024

OCA at 16:59, 15 December 2021

OCA at 04:45, 7 April 2021

OCA: Protected "7ntp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ntp is ON HOLD Authors: Description: Category: Unreleased Structures