7nzo - Revision history

OCA at 12:42, 1 February 2024

OCA — Thu, 01 Feb 2024 12:42:21 GMT

←Older revision		Revision as of 12:42, 1 February 2024
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	==D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp==		==D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp==
-	<StructureSection load='7nzo' size='340' side='right'caption='[[7nzo]]' scene=''>	+	<StructureSection load='7nzo' size='340' side='right'caption='[[7nzo]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NZO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7nzo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermofilum_sp._ex4484_79 Thermofilum sp. ex4484_79]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NZO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nzo OCA], [https://pdbe.org/7nzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nzo RCSB], [https://www.ebi.ac.uk/pdbsum/7nzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nzo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nzo OCA], [https://pdbe.org/7nzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nzo RCSB], [https://www.ebi.ac.uk/pdbsum/7nzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nzo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DLYKI_THEX4 DLYKI_THEX4] Sugar isomerase that catalyzes the reversible isomerization of D-lyxose to D-xylulose (PubMed:34422783). Is highly specific for the substrate D-lyxose, showing less than 2% activity towards mannose and other substrates reported for lyxose isomerases (PubMed:34422783).<ref>PMID:34422783</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel D-lyxose isomerase has been identified within the genome of a hyperthermophilic archaeon belonging to the Thermofilum species. The enzyme has been cloned and over-expressed in Escherichia coli and biochemically characterised. This enzyme differs from other enzymes of this class in that it is highly specific for the substrate D-lyxose, showing less than 2% activity towards mannose and other substrates reported for lyxose isomerases. This is the most thermoactive and thermostable lyxose isomerase reported to date, showing activity above 95 degrees C and retaining 60% of its activity after 60 min incubation at 80 degrees C. This lyxose isomerase is stable in the presence of 50% (v/v) of solvents ethanol, methanol, acetonitrile and DMSO. The crystal structure of the enzyme has been resolved to 1.4-1.7 A. resolution in the ligand-free form and in complexes with both of the slowly reacting sugar substrates mannose and fructose. This thermophilic lyxose isomerase is stabilised by a disulfide bond between the two monomers of the dimeric enzyme and increased hydrophobicity at the dimer interface. These overall properties of high substrate specificity, thermostability and solvent tolerance make this lyxose isomerase enzyme a good candidate for potential industrial applications.
		+
		+	Biochemical and Structural Characterisation of a Novel D-Lyxose Isomerase From the Hyperthermophilic Archaeon Thermofilum sp.,De Rose SA, Kuprat T, Isupov MN, Reinhardt A, Schonheit P, Littlechild JA Front Bioeng Biotechnol. 2021 Aug 6;9:711487. doi: 10.3389/fbioe.2021.711487., eCollection 2021. PMID:34422783<ref>PMID:34422783</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7nzo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Thermofilum sp. ex4484_79]]
	[[Category: De Rose SA]]		[[Category: De Rose SA]]
	[[Category: Isupov MN]]		[[Category: Isupov MN]]
	[[Category: Littlechild JA]]		[[Category: Littlechild JA]]
	[[Category: Schoenheit P]]		[[Category: Schoenheit P]]

OCA at 11:43, 13 October 2021

OCA — Wed, 13 Oct 2021 11:43:36 GMT

←Older revision		Revision as of 11:43, 13 October 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7nzo ~~is ON HOLD until Paper Publication~~	+	==D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp==
-		+	<StructureSection load='7nzo' size='340' side='right'caption='[[7nzo]]' scene=''>
-	~~Authors~~: ~~De Rose~~, S.A., ~~Isupov~~, M.N., ~~Littlechild, J~~.A., ~~Schoenheit, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NZO FirstGlance]. <br>
-	~~Description: D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nzo OCA], [https://pdbe.org/7nzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nzo RCSB], [https://www.ebi.ac.uk/pdbsum/7nzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nzo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Schoenheit, P~~]]	+	__TOC__
-	[[Category: ~~Littlechild, J.A~~]]	+	</StructureSection>
-	[[Category: ~~Isupov, M.N~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~De Rose, S.A~~]]	+	[[Category: De Rose SA]]
		+	[[Category: Isupov MN]]
		+	[[Category: Littlechild JA]]
		+	[[Category: Schoenheit P]]

OCA at 04:23, 28 April 2021

OCA — Wed, 28 Apr 2021 04:23:07 GMT

←Older revision		Revision as of 04:23, 28 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7nzo is ON HOLD	+	The entry 7nzo is ON HOLD until Paper Publication

	Authors: De Rose, S.A., Isupov, M.N., Littlechild, J.A., Schoenheit, P.		Authors: De Rose, S.A., Isupov, M.N., Littlechild, J.A., Schoenheit, P.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Schoenheit, P]]		[[Category: Schoenheit, P]]
-	[[Category: Isupov, M.N]]
	[[Category: Littlechild, J.A]]		[[Category: Littlechild, J.A]]
		+	[[Category: Isupov, M.N]]
	[[Category: De Rose, S.A]]		[[Category: De Rose, S.A]]

OCA: Protected "7nzo" [edit=sysop:move=sysop]

OCA — Wed, 31 Mar 2021 09:34:50 GMT

Protected "7nzo" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:34, 31 March 2021

OCA: New page: '''Unreleased structure''' The entry 7nzo is ON HOLD Authors: De Rose, S.A., Isupov, M.N., Littlechild, J.A., Schoenheit, P. Description: D-lyxose isomerasefrom the hyperthermophilic a...

OCA — Wed, 31 Mar 2021 09:34:49 GMT

New page: '''Unreleased structure''' The entry 7nzo is ON HOLD Authors: De Rose, S.A., Isupov, M.N., Littlechild, J.A., Schoenheit, P. Description: D-lyxose isomerasefrom the hyperthermophilic a...

New page

'''Unreleased structure'''

The entry 7nzo is ON HOLD

Authors: De Rose, S.A., Isupov, M.N., Littlechild, J.A., Schoenheit, P.

Description: D-lyxose isomerasefrom the hyperthermophilic archaeon Thermofilum sp
[[Category: Unreleased Structures]]
[[Category: Schoenheit, P]]
[[Category: Isupov, M.N]]
[[Category: Littlechild, J.A]]
[[Category: De Rose, S.A]]