7o1r - Revision history

OCA at 12:43, 1 February 2024

OCA — Thu, 01 Feb 2024 12:43:17 GMT

←Older revision		Revision as of 12:43, 1 February 2024
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	<StructureSection load='7o1r' size='340' side='right'caption='[[7o1r]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='7o1r' size='340' side='right'caption='[[7o1r]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7o1r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O1R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypoxylon_sp._EC38 Hypoxylon sp. EC38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O1R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Unspecific_peroxygenase Unspecific peroxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.1 1.11.2.1] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o1r OCA], [https://pdbe.org/7o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o1r RCSB], [https://www.ebi.ac.uk/pdbsum/7o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o1r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o1r OCA], [https://pdbe.org/7o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o1r RCSB], [https://www.ebi.ac.uk/pdbsum/7o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o1r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1Y2TH07_9PEZI A0A1Y2TH07_9PEZI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hypoxylon sp. EC38]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Unspecific peroxygenase]]~~	+	[[Category: Mattevi A]]
-	[[Category: Mattevi, A]]	+	[[Category: Rotilio L]]
-	[[Category: Rotilio, L]]	+
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Peroxygenase~~]]	+

OCA at 09:04, 29 September 2021

OCA — Wed, 29 Sep 2021 09:04:32 GMT

←Older revision		Revision as of 09:04, 29 September 2021
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	==Unspecific peroxygenase from Hypoxylon sp. EC38 in complex with imidazole==		==Unspecific peroxygenase from Hypoxylon sp. EC38 in complex with imidazole==
-	<StructureSection load='7o1r' size='340' side='right'caption='[[7o1r]]' scene=''>	+	<StructureSection load='7o1r' size='340' side='right'caption='[[7o1r]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O1R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o1r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O1R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o1r OCA], [https://pdbe.org/7o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o1r RCSB], [https://www.ebi.ac.uk/pdbsum/7o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o1r ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Unspecific_peroxygenase Unspecific peroxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.2.1 1.11.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o1r OCA], [https://pdbe.org/7o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o1r RCSB], [https://www.ebi.ac.uk/pdbsum/7o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o1r ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unspecific peroxygenases (UPO) are glycosylated fungal enzymes that can selectively oxidize C-H bonds. UPOs employ hydrogen peroxide as oxygen donor and reductant. With such an easy-to-handle co-substrate and without the need of a reducing agent, UPOs are emerging as convenient oxidative biocatalysts. Here, an unspecific peroxygenase from Hypoxylon sp. EC38 (HspUPO) was identified in an activity-based screen of six putative peroxygenase enzymes that were heterologously expressed in Pichia pastoris. The enzyme was found to tolerate selected organic solvents such as acetonitrile and acetone. HspUPO is a versatile catalyst performing various reactions, such as the oxidation of prim- and sec-alcohols, epoxidations and hydroxylations. Semi-preparative biotransformations were demonstrated for the non-enantioselective oxidation of racemic 1-phenylethanol rac -1b (TON = 13000), giving the product with 88% isolated yield, and the oxidation of indole 6a to give indigo 6b (TON = 2800) with 98% isolated yield. HspUPO features a compact and rigid three-dimensional conformation that wraps around the heme and defines a funnel-shaped tunnel that leads to the heme iron from the protein surface. The tunnel extends along a distance of about 12 A with a fairly constant diameter in its innermost segment. Its surface comprises both hydrophobic and hydrophilic groups for dealing with small-to-medium size substrates of variable polarities. The structural investigation of several protein-ligand complexes revealed that the active site of HspUPO is accessible to molecules of varying bulkiness and polarity with minimal or no conformational changes, explaining the relatively broad substrate scope of the enzyme. With its convenient expression system, robust operational properties, relatively small size, well-defined structural features, and diverse reaction scope, HspUPO is an exploitable candidate for peroxygenase-based biocatalysis.
		+
		+	Structural and biochemical studies enlighten the unspecific peroxygenase from Hypoxylon sp. EC38 as an efficient oxidative biocatalyst.,Rotilio L, Swoboda A, Ebner K, Rinnofner C, Glieder A, Kroutil W, Mattevi A ACS Catal. 2021 Sep 17;11(18):11511-11525. doi: 10.1021/acscatal.1c03065. Epub, 2021 Sep 2. PMID:34540338<ref>PMID:34540338</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o1r" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mattevi A]]	+	[[Category: Unspecific peroxygenase]]
-	[[Category: Rotilio L]]	+	[[Category: Mattevi, A]]
		+	[[Category: Rotilio, L]]
		+	[[Category: Heme]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Peroxygenase]]

OCA at 09:28, 15 September 2021

OCA — Wed, 15 Sep 2021 09:28:02 GMT

←Older revision		Revision as of 09:28, 15 September 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7o1r ~~is ON HOLD until Paper Publication~~	+	==Unspecific peroxygenase from Hypoxylon sp. EC38 in complex with imidazole==
-		+	<StructureSection load='7o1r' size='340' side='right'caption='[[7o1r]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O1R FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o1r OCA], [https://pdbe.org/7o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o1r RCSB], [https://www.ebi.ac.uk/pdbsum/7o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o1r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mattevi A]]
		+	[[Category: Rotilio L]]

OCA: Protected "7o1r" [edit=sysop:move=sysop]

OCA — Wed, 07 Apr 2021 04:52:58 GMT

Protected "7o1r" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:52, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o1r is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Apr 2021 04:52:57 GMT

New page: '''Unreleased structure''' The entry 7o1r is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7o1r is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]