7o4u - Revision history

OCA at 12:44, 1 February 2024

OCA — Thu, 01 Feb 2024 12:44:43 GMT

←Older revision		Revision as of 12:44, 1 February 2024
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	<StructureSection load='7o4u' size='340' side='right'caption='[[7o4u]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='7o4u' size='340' side='right'caption='[[7o4u]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7o4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4U FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAD:NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>fadB, Rv0860 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=83332 MYCTU])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/3~~-~~hydroxyacyl~~-~~CoA_dehydrogenase 3-hydroxyacyl-CoA dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.35 1.1.1.35]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4u OCA], [https://pdbe.org/7o4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4u RCSB], [https://www.ebi.ac.uk/pdbsum/7o4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4u OCA], [https://pdbe.org/7o4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4u RCSB], [https://www.ebi.ac.uk/pdbsum/7o4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O53872_MYCTU O53872_MYCTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7o4u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7o4u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl-CoA dehydrogenase 3D structures\|Acyl-CoA dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 3-hydroxyacyl-CoA dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Dalwani, S]]	+	[[Category: Dalwani S]]
-	[[Category: Venkatesan, R]]	+	[[Category: Venkatesan R]]
-	[[Category: Wierenga~~, R K]]~~	+	[[Category: Wierenga RK]]
-	~~[[Category: Fatty acid beta oxidation]]~~	+
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Trifunctional enzyme~~]]	+

OCA at 07:03, 22 September 2021

OCA — Wed, 22 Sep 2021 07:03:56 GMT

←Older revision		Revision as of 07:03, 22 September 2021
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	==Structure of the alpha subunit of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide==		==Structure of the alpha subunit of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide==
-	<StructureSection load='7o4u' size='340' side='right'caption='[[7o4u]]' scene=''>	+	<StructureSection load='7o4u' size='340' side='right'caption='[[7o4u]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4u OCA], [https://pdbe.org/7o4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4u RCSB], [https://www.ebi.ac.uk/pdbsum/7o4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4u ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fadB, Rv0860 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-hydroxyacyl-CoA_dehydrogenase 3-hydroxyacyl-CoA dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.35 1.1.1.35] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4u OCA], [https://pdbe.org/7o4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4u RCSB], [https://www.ebi.ac.uk/pdbsum/7o4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4u ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Mycobacterium tuberculosis trifunctional enzyme (MtTFE) is an alpha2beta2 tetrameric enzyme. The alpha-chain harbors the 2E-enoyl-CoA hydratase (ECH) and 3S-hydroxyacyl-CoA dehydrogenase (HAD) activities and the beta-chain provides the 3-ketoacyl-CoA thiolase (KAT) activity. Enzyme kinetic data reported here show that medium and long chain enoyl-CoA molecules are preferred substrates for MtTFE. Modelling studies indicate how the linear medium and long acyl chains of these substrates can bind to each of the active sites. In addition, crystallographic binding studies have identified three new CoA binding sites which are different from the previously known CoA binding sites of the three TFE active sites. Structure comparisons provide new insights into the properties of ECH, HAD and KAT active sites of MtTFE. The interactions of the adenine moiety of CoA with loop-2 of the ECH active site cause a conformational change of this loop by which a competent ECH active site is formed. The NAD(+) binding domain (domain C) of the HAD part of MtTFE has only a few interactions with the rest of the complex and adopts a range of open conformations, whereas the A-domain of the ECH part is rigidly fixed with respect to the HAD part. Two loops, the CB1-CA1 region and the catalytic CB4-CB5 loop, near the thiolase active site and the thiolase dimer interface, have high B-factors. Structure comparisons suggest that a competent and stable thiolase dimer is formed only when complexed with the alpha-chains, highlighting the importance of the assembly for the proper functioning of the complex.
		+
		+	Substrate specificity and conformational flexibility properties of the Mycobacterium tuberculosis beta-oxidation trifunctional enzyme.,Dalwani S, Lampela O, Leprovost P, Schmitz W, Juffer AH, Wierenga RK, Venkatesan R J Struct Biol. 2021 Aug 8;213(3):107776. doi: 10.1016/j.jsb.2021.107776. PMID:34371166<ref>PMID:34371166</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o4u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: 3-hydroxyacyl-CoA dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dalwani S]]	+	[[Category: Myctu]]
-	[[Category: Venkatesan R]]	+	[[Category: Dalwani, S]]
-	[[Category: Wierenga RK]]	+	[[Category: Venkatesan, R]]
		+	[[Category: Wierenga, R K]]
		+	[[Category: Fatty acid beta oxidation]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Trifunctional enzyme]]

OCA at 06:20, 25 August 2021

OCA — Wed, 25 Aug 2021 06:20:42 GMT

←Older revision		Revision as of 06:20, 25 August 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7o4u is ON HOLD until Paper Publication~~	+	==Structure of the alpha subunit of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide==
-		+	<StructureSection load='7o4u' size='340' side='right'caption='[[7o4u]]' scene=''>
-	~~Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4U FirstGlance]. <br>
-	~~Description:~~ Structure of the alpha subunit of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4u OCA], [https://pdbe.org/7o4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4u RCSB], [https://www.ebi.ac.uk/pdbsum/7o4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Dalwani, S]]	+	__TOC__
-	[[Category: ~~Wierenga,~~ R.K]]	+	</StructureSection>
-	[[Category: ~~Venkatesan, R~~]]	+	[[Category: Large Structures]]
		+	[[Category: Dalwani S]]
		+	[[Category: Venkatesan R]]
		+	[[Category: Wierenga RK]]

OCA at 07:34, 5 May 2021

OCA — Wed, 05 May 2021 07:34:17 GMT

←Older revision		Revision as of 07:34, 5 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o4u is ON HOLD	+	The entry 7o4u is ON HOLD until Paper Publication

	Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R.		Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R.

OCA: Protected "7o4u" [edit=sysop:move=sysop]

OCA — Wed, 14 Apr 2021 05:50:35 GMT

Protected "7o4u" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 14 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o4u is ON HOLD Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R. Description: Structure of the alpha subunit of Mycobacterium tuberculosis beta...

OCA — Wed, 14 Apr 2021 05:50:34 GMT

New page: '''Unreleased structure''' The entry 7o4u is ON HOLD Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R. Description: Structure of the alpha subunit of Mycobacterium tuberculosis beta...

New page

'''Unreleased structure'''

The entry 7o4u is ON HOLD

Authors: Dalwani, S., Wierenga, R.K., Venkatesan, R.

Description: Structure of the alpha subunit of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide
[[Category: Unreleased Structures]]
[[Category: Dalwani, S]]
[[Category: Wierenga, R.K]]
[[Category: Venkatesan, R]]