7o67 - Revision history

OCA at 12:45, 1 February 2024

OCA — Thu, 01 Feb 2024 12:45:29 GMT

←Older revision		Revision as of 12:45, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7o67' size='340' side='right'caption='[[7o67]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='7o67' size='340' side='right'caption='[[7o67]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7o67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O67 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[7o63\|7o63]], [[7o64\|7o64]], [[7o65\|7o65]], [[7o66\|7o66]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o67 OCA], [https://pdbe.org/7o67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o67 RCSB], [https://www.ebi.ac.uk/pdbsum/7o67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o67 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o67 OCA], [https://pdbe.org/7o67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o67 RCSB], [https://www.ebi.ac.uk/pdbsum/7o67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FTMT_HUMAN FTMT_HUMAN]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.<ref>PMID:11323407</ref> <ref>PMID:15201052</ref>	+	[https://www.uniprot.org/uniprot/FTMT_HUMAN FTMT_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.<ref>PMID:11323407</ref> <ref>PMID:15201052</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ferroxidase~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ciambellotti, S]]	+	[[Category: Ciambellotti S]]
-	[[Category: Mangani, S]]	+	[[Category: Mangani S]]
-	[[Category: Pozzi, C]]	+	[[Category: Pozzi C]]
-	[[Category: Tassone, G]]	+	[[Category: Tassone G]]
-	[[Category: Turano, P]]	+	[[Category: Turano P]]
-	~~[[Category: Dioxygen]]~~	+
-	~~[[Category: Ferroxidase site]]~~	+
-	~~[[Category: Hmtf]]~~	+
-	~~[[Category: Human mitochondrial ferritin]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Superoxide anion]]~~	+
-	~~[[Category: Time-controlled iron loading~~]]	+

OCA at 16:01, 3 November 2021

OCA — Wed, 03 Nov 2021 16:01:19 GMT

←Older revision		Revision as of 16:01, 3 November 2021
Line 1:		Line 1:

	==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==		==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==
-	<StructureSection load='7o67' size='340' side='right'caption='[[7o67]]' scene=''>	+	<StructureSection load='7o67' size='340' side='right'caption='[[7o67]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O67 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o67 OCA], [https://pdbe.org/7o67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o67 RCSB], [https://www.ebi.ac.uk/pdbsum/7o67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o67 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7o63\|7o63]], [[7o64\|7o64]], [[7o65\|7o65]], [[7o66\|7o66]]</div></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o67 OCA], [https://pdbe.org/7o67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o67 RCSB], [https://www.ebi.ac.uk/pdbsum/7o67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o67 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FTMT_HUMAN FTMT_HUMAN]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.<ref>PMID:11323407</ref> <ref>PMID:15201052</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ferritins are nanocage proteins that store iron ions in their central cavity as hydrated ferric oxide biominerals. In mammals, further the L (light) and H (heavy) chains constituting cytoplasmic maxi-ferritins, an additional type of ferritin has been identified, the mitochondrial ferritin (MTF). Human MTF (hMTF) is a functional homopolymeric H-like ferritin performing the ferroxidase activity in its ferroxidase site (FS), in which Fe(II) is oxidized to Fe(III) in the presence of dioxygen. To better investigate its ferroxidase properties, here we performed time-lapse X-ray crystallography analysis of hMTF, providing structural evidence of how iron ions interact with hMTF and of their binding to the FS. Transient iron binding sites, populating the pathway along the cage from the iron entry channel to the catalytic center, were also identified. Furthermore, our kinetic data at variable iron loads indicate that the catalytic iron oxidation reaction occurs via a diferric peroxo intermediate followed by the formation of ferric-oxo species, with significant differences with respect to human H-type ferritin.
		+
		+	Iron Binding in the Ferroxidase Site of Human Mitochondrial Ferritin.,Ciambellotti S, Pratesi A, Tassone G, Turano P, Mangani S, Pozzi C Chemistry. 2021 Aug 3. doi: 10.1002/chem.202102270. PMID:34343376<ref>PMID:34343376</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o67" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ferroxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ciambellotti S]]	+	[[Category: Ciambellotti, S]]
-	[[Category: Mangani S]]	+	[[Category: Mangani, S]]
-	[[Category: Pozzi C]]	+	[[Category: Pozzi, C]]
-	[[Category: Tassone G]]	+	[[Category: Tassone, G]]
-	[[Category: Turano P]]	+	[[Category: Turano, P]]
		+	[[Category: Dioxygen]]
		+	[[Category: Ferroxidase site]]
		+	[[Category: Hmtf]]
		+	[[Category: Human mitochondrial ferritin]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Superoxide anion]]
		+	[[Category: Time-controlled iron loading]]

OCA at 11:44, 13 October 2021

OCA — Wed, 13 Oct 2021 11:44:04 GMT

←Older revision		Revision as of 11:44, 13 October 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7o67 is ON HOLD~~	+	==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==
-		+	<StructureSection load='7o67' size='340' side='right'caption='[[7o67]]' scene=''>
-	~~Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O67 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o67 OCA], [https://pdbe.org/7o67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o67 RCSB], [https://www.ebi.ac.uk/pdbsum/7o67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o67 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Mangani,~~ S]]	+	__TOC__
-	[[Category: ~~Ciambellotti,~~ S]]	+	</StructureSection>
-	[[Category: ~~Turano, P~~]]	+	[[Category: Large Structures]]
-	[[Category: Tassone, G]]	+	[[Category: Ciambellotti S]]
-	[[Category: ~~Pozzi, C~~]]	+	[[Category: Mangani S]]
		+	[[Category: Pozzi C]]
		+	[[Category: Tassone G]]
		+	[[Category: Turano P]]

OCA: Protected "7o67" [edit=sysop:move=sysop]

OCA — Wed, 21 Apr 2021 06:37:17 GMT

Protected "7o67" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:37, 21 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o67 is ON HOLD Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S. Description: Crystal structure of human mitochondrial fe...

OCA — Wed, 21 Apr 2021 06:37:15 GMT

New page: '''Unreleased structure''' The entry 7o67 is ON HOLD Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S. Description: Crystal structure of human mitochondrial fe...

New page

'''Unreleased structure'''

The entry 7o67 is ON HOLD

Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.

Description: Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 15 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site
[[Category: Unreleased Structures]]
[[Category: Mangani, S]]
[[Category: Ciambellotti, S]]
[[Category: Turano, P]]
[[Category: Tassone, G]]
[[Category: Pozzi, C]]