7o68 - Revision history

OCA at 12:45, 1 February 2024

OCA — Thu, 01 Feb 2024 12:45:31 GMT

←Older revision		Revision as of 12:45, 1 February 2024
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	==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 120 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==		==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 120 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==
-	<StructureSection load='7o68' size='340' side='right'caption='[[7o68]]' scene=''>	+	<StructureSection load='7o68' size='340' side='right'caption='[[7o68]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O68 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o68 OCA], [https://pdbe.org/7o68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o68 RCSB], [https://www.ebi.ac.uk/pdbsum/7o68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o68 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.68Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o68 OCA], [https://pdbe.org/7o68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o68 RCSB], [https://www.ebi.ac.uk/pdbsum/7o68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o68 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FTMT_HUMAN FTMT_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.<ref>PMID:11323407</ref> <ref>PMID:15201052</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ferritins are nanocage proteins that store iron ions in their central cavity as hydrated ferric oxide biominerals. In mammals, further the L (light) and H (heavy) chains constituting cytoplasmic maxi-ferritins, an additional type of ferritin has been identified, the mitochondrial ferritin (MTF). Human MTF (hMTF) is a functional homopolymeric H-like ferritin performing the ferroxidase activity in its ferroxidase site (FS), in which Fe(II) is oxidized to Fe(III) in the presence of dioxygen. To better investigate its ferroxidase properties, here we performed time-lapse X-ray crystallography analysis of hMTF, providing structural evidence of how iron ions interact with hMTF and of their binding to the FS. Transient iron binding sites, populating the pathway along the cage from the iron entry channel to the catalytic center, were also identified. Furthermore, our kinetic data at variable iron loads indicate that the catalytic iron oxidation reaction occurs via a diferric peroxo intermediate followed by the formation of ferric-oxo species, with significant differences with respect to human H-type ferritin.
		+
		+	Iron Binding in the Ferroxidase Site of Human Mitochondrial Ferritin.,Ciambellotti S, Pratesi A, Tassone G, Turano P, Mangani S, Pozzi C Chemistry. 2021 Aug 3. doi: 10.1002/chem.202102270. PMID:34343376<ref>PMID:34343376</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o68" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ciambellotti S]]		[[Category: Ciambellotti S]]

OCA at 11:44, 13 October 2021

OCA — Wed, 13 Oct 2021 11:44:04 GMT

←Older revision		Revision as of 11:44, 13 October 2021
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-	'''Unreleased structure'''

-	~~The entry 7o68 is ON HOLD until Paper Publication~~	+	==Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 120 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site==
-		+	<StructureSection load='7o68' size='340' side='right'caption='[[7o68]]' scene=''>
-	~~Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O68 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 120 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o68 OCA], [https://pdbe.org/7o68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o68 RCSB], [https://www.ebi.ac.uk/pdbsum/7o68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o68 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Mangani,~~ S]]	+	__TOC__
-	[[Category: ~~Ciambellotti,~~ S]]	+	</StructureSection>
-	[[Category: ~~Turano, P~~]]	+	[[Category: Large Structures]]
-	[[Category: Tassone, G]]	+	[[Category: Ciambellotti S]]
-	[[Category: ~~Pozzi, C~~]]	+	[[Category: Mangani S]]
		+	[[Category: Pozzi C]]
		+	[[Category: Tassone G]]
		+	[[Category: Turano P]]

OCA at 08:19, 12 May 2021

OCA — Wed, 12 May 2021 08:19:40 GMT

←Older revision		Revision as of 08:19, 12 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o68 is ON HOLD	+	The entry 7o68 is ON HOLD until Paper Publication

	Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.		Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.

OCA: Protected "7o68" [edit=sysop:move=sysop]

OCA — Wed, 21 Apr 2021 06:37:21 GMT

Protected "7o68" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:37, 21 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o68 is ON HOLD Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S. Description: Crystal structure of human mitochondrial fe...

OCA — Wed, 21 Apr 2021 06:37:20 GMT

New page: '''Unreleased structure''' The entry 7o68 is ON HOLD Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S. Description: Crystal structure of human mitochondrial fe...

New page

'''Unreleased structure'''

The entry 7o68 is ON HOLD

Authors: Pozzi, C., Ciambellotti, S., Tassone, G., Turano, P., Mangani, S.

Description: Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 120 minutes showing either a dioxygen or a superoxide anion coordinated to iron ions in the ferroxidase site
[[Category: Unreleased Structures]]
[[Category: Mangani, S]]
[[Category: Ciambellotti, S]]
[[Category: Turano, P]]
[[Category: Tassone, G]]
[[Category: Pozzi, C]]