7o9f - Revision history

OCA at 12:47, 1 February 2024

OCA — Thu, 01 Feb 2024 12:47:44 GMT

←Older revision		Revision as of 12:47, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7o9f' size='340' side='right'caption='[[7o9f]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='7o9f' size='340' side='right'caption='[[7o9f]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7o9f]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o9f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.51Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9f OCA], [https://pdbe.org/7o9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9f RCSB], [https://www.ebi.ac.uk/pdbsum/7o9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9f OCA], [https://pdbe.org/7o9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9f RCSB], [https://www.ebi.ac.uk/pdbsum/7o9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SRP54_BACSU SRP54_BACSU]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).<ref>PMID:7511896</ref>	+	[https://www.uniprot.org/uniprot/SRP54_BACSU SRP54_BACSU] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).<ref>PMID:7511896</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7o9f" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7o9f" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Signal recognition particle 3D structures\|Signal recognition particle 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bange, G]]	+	[[Category: Bange G]]
-	[[Category: Czech, L]]	+	[[Category: Czech L]]
-	[[Category: Mais, C N]]	+	[[Category: Mais C-N]]
-	~~[[Category: Alarmone]]~~	+
-	~~[[Category: Signal recognition particle]]~~	+
-	~~[[Category: Stress]]~~	+
-	~~[[Category: Stringent response]]~~	+
-	~~[[Category: Targeting complex]]~~	+
-	~~[[Category: Translation~~]]	+

OCA at 07:23, 9 March 2022

OCA — Wed, 09 Mar 2022 07:23:34 GMT

←Older revision		Revision as of 07:23, 9 March 2022
Line 1:		Line 1:

	==Bacillus subtilis Ffh in complex with ppGpp==		==Bacillus subtilis Ffh in complex with ppGpp==
-	<StructureSection load='7o9f' size='340' side='right'caption='[[7o9f]]' scene=''>	+	<StructureSection load='7o9f' size='340' side='right'caption='[[7o9f]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o9f]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9F FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9f OCA], [https://pdbe.org/7o9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9f RCSB], [https://www.ebi.ac.uk/pdbsum/7o9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9f ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9f OCA], [https://pdbe.org/7o9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9f RCSB], [https://www.ebi.ac.uk/pdbsum/7o9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9f ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/SRP54_BACSU SRP54_BACSU]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).<ref>PMID:7511896</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stringent response enables bacteria to respond to nutrient limitation and other stress conditions through production of the nucleotide-based second messengers ppGpp and pppGpp, collectively known as (p)ppGpp. Here, we report that (p)ppGpp inhibits the signal recognition particle (SRP)-dependent protein targeting pathway, which is essential for membrane protein biogenesis and protein secretion. More specifically, (p)ppGpp binds to the SRP GTPases Ffh and FtsY, and inhibits the formation of the SRP receptor-targeting complex, which is central for the coordinated binding of the translating ribosome to the SecYEG translocon. Cryo-EM analysis of SRP bound to translating ribosomes suggests that (p)ppGpp may induce a distinct conformational stabilization of the NG domain of Ffh and FtsY in Bacillus subtilis but not in E. coli.
		+
		+	Inhibition of SRP-dependent protein secretion by the bacterial alarmone (p)ppGpp.,Czech L, Mais CN, Kratzat H, Sarmah P, Giammarinaro P, Freibert SA, Esser HF, Musial J, Berninghausen O, Steinchen W, Beckmann R, Koch HG, Bange G Nat Commun. 2022 Feb 25;13(1):1069. doi: 10.1038/s41467-022-28675-0. PMID:35217658<ref>PMID:35217658</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o9f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bange G]]	+	[[Category: Bange, G]]
-	[[Category: Czech L]]	+	[[Category: Czech, L]]
-	[[Category: Mais C-N]]	+	[[Category: Mais, C N]]
		+	[[Category: Alarmone]]
		+	[[Category: Signal recognition particle]]
		+	[[Category: Stress]]
		+	[[Category: Stringent response]]
		+	[[Category: Targeting complex]]
		+	[[Category: Translation]]

OCA at 07:00, 2 February 2022

OCA — Wed, 02 Feb 2022 07:00:51 GMT

←Older revision		Revision as of 07:00, 2 February 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7o9f ~~is ON HOLD until Paper Publication~~	+	==Bacillus subtilis Ffh in complex with ppGpp==
-		+	<StructureSection load='7o9f' size='340' side='right'caption='[[7o9f]]' scene=''>
-	~~Authors~~: ~~Czech~~, L., ~~Mais~~, C.-N., ~~Bange~~, G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9F FirstGlance]. <br>
-	~~Description: Bacillus subtilis Ffh in complex with ppGpp~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9f OCA], [https://pdbe.org/7o9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9f RCSB], [https://www.ebi.ac.uk/pdbsum/7o9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9f ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Bange, G]]	+	__TOC__
-	[[Category: Czech, L]]	+	</StructureSection>
-	[[Category: Mais, C.-N]]	+	[[Category: Large Structures]]
		+	[[Category: Bange G]]
		+	[[Category: Czech L]]
		+	[[Category: Mais C-N]]

OCA at 09:02, 19 May 2021

OCA — Wed, 19 May 2021 09:02:32 GMT

←Older revision		Revision as of 09:02, 19 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o9f is ON HOLD	+	The entry 7o9f is ON HOLD until Paper Publication

	Authors: Czech, L., Mais, C.-N., Bange, G.		Authors: Czech, L., Mais, C.-N., Bange, G.

OCA: Protected "7o9f" [edit=sysop:move=sysop]

OCA — Wed, 21 Apr 2021 06:47:15 GMT

Protected "7o9f" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:47, 21 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o9f is ON HOLD Authors: Czech, L., Mais, C.-N., Bange, G. Description: Bacillus subtilis Ffh in complex with ppGpp [[Category: Unreleased Structur...

OCA — Wed, 21 Apr 2021 06:47:13 GMT

New page: '''Unreleased structure''' The entry 7o9f is ON HOLD Authors: Czech, L., Mais, C.-N., Bange, G. Description: Bacillus subtilis Ffh in complex with ppGpp [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 7o9f is ON HOLD

Authors: Czech, L., Mais, C.-N., Bange, G.

Description: Bacillus subtilis Ffh in complex with ppGpp
[[Category: Unreleased Structures]]
[[Category: Bange, G]]
[[Category: Czech, L]]
[[Category: Mais, C.-N]]