7o9s - Revision history

OCA at 12:47, 1 February 2024

OCA — Thu, 01 Feb 2024 12:47:54 GMT

←Older revision		Revision as of 12:47, 1 February 2024
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-	====	+	==Hantaan virus Gn in complex with Fab nnHTN-Gn2==
-	<StructureSection load='7o9s' size='340' side='right'caption='[[7o9s]]' scene=''>	+	<StructureSection load='7o9s' size='340' side='right'caption='[[7o9s]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o9s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hantaan_orthohantavirus Hantaan orthohantavirus] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9s OCA], [https://pdbe.org/7o9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9s RCSB], [https://www.ebi.ac.uk/pdbsum/7o9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9s OCA], [https://pdbe.org/7o9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9s RCSB], [https://www.ebi.ac.uk/pdbsum/7o9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A077D153_9VIRU A0A077D153_9VIRU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hantaviruses are a group of emerging pathogens capable of causing severe disease upon zoonotic transmission to humans. The mature hantavirus surface presents higher-order tetrameric assemblies of two glycoproteins, Gn and Gc, which are responsible for negotiating host cell entry and constitute key therapeutic targets. Here, we demonstrate that recombinantly derived Gn from Hantaan virus (HTNV) elicits a neutralizing antibody response (serum dilution that inhibits 50% infection [ID(50)], 1:200 to 1:850) in an animal model. Using antigen-specific B cell sorting, we isolated monoclonal antibodies (mAbs) exhibiting neutralizing and non-neutralizing activity, termed mAb HTN-Gn1 and mAb nnHTN-Gn2, respectively. Crystallographic analysis reveals that these mAbs target spatially distinct epitopes at disparate sites of the N-terminal region of the HTNV Gn ectodomain. Epitope mapping onto a model of the higher order (Gn-Gc)(4) spike supports the immune accessibility of the mAb HTN-Gn1 epitope, a hypothesis confirmed by electron cryo-tomography of the antibody with virus-like particles. These data define natively exposed regions of the hantaviral Gn that can be targeted in immunogen design. IMPORTANCE The spillover of pathogenic hantaviruses from rodent reservoirs into the human population poses a continued threat to human health. Here, we show that a recombinant form of the Hantaan virus (HTNV) surface-displayed glycoprotein, Gn, elicits a neutralizing antibody response in rabbits. We isolated a neutralizing (HTN-Gn1) and a non-neutralizing (nnHTN-Gn2) monoclonal antibody and provide the first molecular-level insights into how the Gn glycoprotein may be targeted by the antibody-mediated immune response. These findings may guide rational vaccine design approaches focused on targeting the hantavirus glycoprotein envelope.
		+
		+	Structural Basis for a Neutralizing Antibody Response Elicited by a Recombinant Hantaan Virus Gn Immunogen.,Rissanen I, Krumm SA, Stass R, Whitaker A, Voss JE, Bruce EA, Rothenberger S, Kunz S, Burton DR, Huiskonen JT, Botten JW, Bowden TA, Doores KJ mBio. 2021 Aug 31;12(4):e0253120. doi: 10.1128/mBio.02531-20. Epub 2021 Jul 6. PMID:34225492<ref>PMID:34225492</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o9s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hantaan orthohantavirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Oryctolagus cuniculus]]
		+	[[Category: Bowden TA]]
		+	[[Category: Huiskonen JT]]
		+	[[Category: Rissanen I]]
		+	[[Category: Stass R]]

OCA at 07:33, 25 June 2021

OCA — Fri, 25 Jun 2021 07:33:46 GMT

←Older revision		Revision as of 07:33, 25 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7o9s ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7o9s' size='340' side='right'caption='[[7o9s]]' scene=''>
-	~~Authors~~: ~~Rissanen, I~~.~~, Bowden, T~~.A.~~, Huiskonen, J~~.T.~~, Stass, R~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Hantaan virus Gn in complex with Fab nnHTN-Gn2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9s OCA], [https://pdbe.org/7o9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9s RCSB], [https://www.ebi.ac.uk/pdbsum/7o9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9s ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Stass~~, R]]	+	__TOC__
-	[~~[Category~~: ~~Rissanen, I~~]]	+	</StructureSection>
-	[[Category: ~~Huiskonen, J.T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bowden, T.A~~]]	+	[[Category: Z-disk]]

OCA at 09:02, 19 May 2021

OCA — Wed, 19 May 2021 09:02:55 GMT

←Older revision		Revision as of 09:02, 19 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o9s is ON HOLD	+	The entry 7o9s is ON HOLD until Paper Publication

	Authors: Rissanen, I., Bowden, T.A., Huiskonen, J.T., Stass, R.		Authors: Rissanen, I., Bowden, T.A., Huiskonen, J.T., Stass, R.

OCA: Protected "7o9s" [edit=sysop:move=sysop]

OCA — Wed, 28 Apr 2021 04:25:51 GMT

Protected "7o9s" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 28 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o9s is ON HOLD Authors: Rissanen, I., Bowden, T.A., Huiskonen, J.T., Stass, R. Description: Hantaan virus Gn in complex with Fab nnHTN-Gn2 [[Categ...

OCA — Wed, 28 Apr 2021 04:25:50 GMT

New page: '''Unreleased structure''' The entry 7o9s is ON HOLD Authors: Rissanen, I., Bowden, T.A., Huiskonen, J.T., Stass, R. Description: Hantaan virus Gn in complex with Fab nnHTN-Gn2 [[Categ...

New page

'''Unreleased structure'''

The entry 7o9s is ON HOLD

Authors: Rissanen, I., Bowden, T.A., Huiskonen, J.T., Stass, R.

Description: Hantaan virus Gn in complex with Fab nnHTN-Gn2
[[Category: Unreleased Structures]]
[[Category: Stass, R]]
[[Category: Rissanen, I]]
[[Category: Huiskonen, J.T]]
[[Category: Bowden, T.A]]