7ohk - Revision history

OCA at 12:50, 1 February 2024

OCA — Thu, 01 Feb 2024 12:50:48 GMT

←Older revision		Revision as of 12:50, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ohk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ohk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FTSZ_STAA8 FTSZ_STAA8]] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]<ref>PMID:14617148</ref>	+	[https://www.uniprot.org/uniprot/FTSZ_STAA8 FTSZ_STAA8] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]<ref>PMID:14617148</ref>
		+
		+	==See Also==
		+	*[[Cell division protein 3D structures\|Cell division protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:24, 8 September 2022

OCA — Thu, 08 Sep 2022 05:24:07 GMT

←Older revision		Revision as of 05:24, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7ohk' size='340' side='right'caption='[[7ohk]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='7ohk' size='340' side='right'caption='[[7ohk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ohk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ohk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~FTSZ_STAAU FTSZ_STAAU~~]] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]	+	[[https://www.uniprot.org/uniprot/FTSZ_STAA8 FTSZ_STAA8]] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]<ref>PMID:14617148</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Treadmilling protein filaments perform essential cellular functions by growing from one end while shrinking from the other, driven by nucleotide hydrolysis. Bacterial cell division relies on the primitive tubulin homolog FtsZ, a target for antibiotic discovery that assembles into single treadmilling filaments that hydrolyse GTP at an active site formed upon subunit association. We determined high-resolution filament structures of FtsZ from the pathogen Staphylococcus aureus in complex with different nucleotide analogs and cations, including mimetics of the ground and transition states of catalysis. Together with mutational and biochemical analyses, our structures reveal interactions made by the GTP gamma-phosphate and Mg2+ at the subunit interface, a K+ ion stabilizing loop T7 for co-catalysis, new roles of key residues at the active site and a nearby crosstalk area, and rearrangements of a dynamic water shell bridging adjacent subunits upon GTP hydrolysis. We propose a mechanistic model that integrates nucleotide hydrolysis signaling with assembly-associated conformational changes and filament treadmilling. Equivalent assembly mechanisms may apply to more complex tubulin and actin cytomotive filaments that share analogous features with FtsZ.	+
-		+
-	FtsZ filament structures in different nucleotide states reveal the mechanism of assembly dynamics.,Ruiz FM, Huecas S, Santos-Aledo A, Prim EA, Andreu JM, Fernandez-Tornero C PLoS Biol. 2022 Mar 21;20(3):e3001497. doi: 10.1371/journal.pbio.3001497., eCollection 2022 Mar. PMID:35312677<ref>PMID:~~35312677~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7ohk" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 14:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Andreu, J M~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Fernandez-Tornero, C~~]]	+	[[Category: Andreu JM]]
-	[[Category: ~~Ruiz, F M~~]]	+	[[Category: Fernandez-Tornero C]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Ruiz FM]]
-	~~[[Category: Cell division protein~~]]	+

OCA at 07:06, 20 July 2022

OCA — Wed, 20 Jul 2022 07:06:14 GMT

←Older revision		Revision as of 07:06, 20 July 2022
Line 1:		Line 1:

	==SaFtsZ complexed with GDP, BeF3- and Mg2+==		==SaFtsZ complexed with GDP, BeF3- and Mg2+==
-	<StructureSection load='7ohk' size='340' side='right'caption='[[7ohk]]' scene=''>	+	<StructureSection load='7ohk' size='340' side='right'caption='[[7ohk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ohk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FTSZ_STAAU FTSZ_STAAU]] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.[HAMAP-Rule:MF_00909]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Treadmilling protein filaments perform essential cellular functions by growing from one end while shrinking from the other, driven by nucleotide hydrolysis. Bacterial cell division relies on the primitive tubulin homolog FtsZ, a target for antibiotic discovery that assembles into single treadmilling filaments that hydrolyse GTP at an active site formed upon subunit association. We determined high-resolution filament structures of FtsZ from the pathogen Staphylococcus aureus in complex with different nucleotide analogs and cations, including mimetics of the ground and transition states of catalysis. Together with mutational and biochemical analyses, our structures reveal interactions made by the GTP gamma-phosphate and Mg2+ at the subunit interface, a K+ ion stabilizing loop T7 for co-catalysis, new roles of key residues at the active site and a nearby crosstalk area, and rearrangements of a dynamic water shell bridging adjacent subunits upon GTP hydrolysis. We propose a mechanistic model that integrates nucleotide hydrolysis signaling with assembly-associated conformational changes and filament treadmilling. Equivalent assembly mechanisms may apply to more complex tubulin and actin cytomotive filaments that share analogous features with FtsZ.
		+
		+	FtsZ filament structures in different nucleotide states reveal the mechanism of assembly dynamics.,Ruiz FM, Huecas S, Santos-Aledo A, Prim EA, Andreu JM, Fernandez-Tornero C PLoS Biol. 2022 Mar 21;20(3):e3001497. doi: 10.1371/journal.pbio.3001497., eCollection 2022 Mar. PMID:35312677<ref>PMID:35312677</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ohk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andreu JM]]	+	[[Category: Andreu, J M]]
-	[[Category: Fernandez-Tornero C]]	+	[[Category: Fernandez-Tornero, C]]
-	[[Category: Ruiz FM]]	+	[[Category: Ruiz, F M]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cell division protein]]

OCA at 06:18, 2 March 2022

OCA — Wed, 02 Mar 2022 06:18:58 GMT

←Older revision		Revision as of 06:18, 2 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ohk ~~is ON HOLD until Paper Publication~~	+	==SaFtsZ complexed with GDP, BeF3- and Mg2+==
-		+	<StructureSection load='7ohk' size='340' side='right'caption='[[7ohk]]' scene=''>
-	~~Authors~~: ~~Fernandez~~-~~Tornero~~, C., ~~Ruiz~~, F.M., ~~Andreu~~, J.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHK FirstGlance]. <br>
-	~~Description: SaFtsZ complexed with GDP, BeF3-and Mg2+~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohk OCA], [https://pdbe.org/7ohk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohk RCSB], [https://www.ebi.ac.uk/pdbsum/7ohk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohk ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Fernandez-Tornero, C~~]]	+	__TOC__
-	[[Category: ~~Andreu, J.M~~]]	+	</StructureSection>
-	[[Category: Ruiz~~, F.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Andreu JM]]
		+	[[Category: Fernandez-Tornero C]]
		+	[[Category: Ruiz FM]]

OCA: Protected "7ohk" [edit=sysop:move=sysop]

OCA — Wed, 19 May 2021 09:06:24 GMT

Protected "7ohk" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:06, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7ohk is ON HOLD until Paper Publication Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M. Description: SaFtsZ complexed with GDP, BeF3-and M...

OCA — Wed, 19 May 2021 09:06:23 GMT

New page: '''Unreleased structure''' The entry 7ohk is ON HOLD until Paper Publication Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M. Description: SaFtsZ complexed with GDP, BeF3-and M...

New page

'''Unreleased structure'''

The entry 7ohk is ON HOLD until Paper Publication

Authors: Fernandez-Tornero, C., Ruiz, F.M., Andreu, J.M.

Description: SaFtsZ complexed with GDP, BeF3-and Mg2+
[[Category: Unreleased Structures]]
[[Category: Fernandez-Tornero, C]]
[[Category: Andreu, J.M]]
[[Category: Ruiz, F.M]]