OCA at 12:54, 1 February 2024

OCA — Thu, 01 Feb 2024 12:54:02 GMT

←Older revision		Revision as of 12:54, 1 February 2024
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	==Bacteroides thetaiotaomicron mannosidase GH2 with beta-manno-configured N-alkyl cyclophellitol aziridine==		==Bacteroides thetaiotaomicron mannosidase GH2 with beta-manno-configured N-alkyl cyclophellitol aziridine==
-	<StructureSection load='7op7' size='340' side='right'caption='[[7op7]]' scene=''>	+	<StructureSection load='7op7' size='340' side='right'caption='[[7op7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7op7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OP7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7op7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7op7 OCA], [https://pdbe.org/7op7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7op7 RCSB], [https://www.ebi.ac.uk/pdbsum/7op7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7op7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=VEE:(1R,2S,3R,4S,5R,6R)-5-(8-azidooctylamino)-6-(hydroxymethyl)cyclohexane-1,2,3,4-tetrol'>VEE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7op7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7op7 OCA], [https://pdbe.org/7op7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7op7 RCSB], [https://www.ebi.ac.uk/pdbsum/7op7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7op7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8AAK6_BACTN Q8AAK6_BACTN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Exo-beta-mannosidases are a broad class of stereochemically retaining hydrolases that are essential for the breakdown of complex carbohydrate substrates found in all kingdoms of life. Yet the detection of exo-beta-mannosidases in complex biological samples remains challenging, necessitating the development of new methodologies. Cyclophellitol and its analogues selectively label the catalytic nucleophiles of retaining glycoside hydrolases, making them valuable tool compounds. Furthermore, cyclophellitol can be readily redesigned to enable the incorporation of a detection tag, generating activity-based probes (ABPs) that can be used to detect and identify specific glycosidases in complex biological samples. Towards the development of ABPs for exo-beta-mannosidases, we present a concise synthesis of beta-manno-configured cyclophellitol, cyclophellitol aziridine, and N-alkyl cyclophellitol aziridines. We show that these probes covalently label exo-beta-mannosidases from GH families 2, 5, and 164. Structural studies of the resulting complexes support a canonical mechanism-based mode of action in which the active site nucleophile attacks the pseudoanomeric centre to form a stable ester linkage, mimicking the glycosyl enzyme intermediate. Furthermore, we demonstrate activity-based protein profiling using an N-alkyl aziridine derivative by specifically labelling MANBA in mouse kidney tissue. Together, these results show that synthetic manno-configured cyclophellitol analogues hold promise for detecting exo-beta-mannosidases in biological and biomedical research.
		+
		+	Synthesis of broad-specificity activity-based probes for exo-beta-mannosidases.,McGregor NGS, Kuo CL, Beenakker TJM, Wong CS, Offen WA, Armstrong Z, Florea BI, Codee JDC, Overkleeft HS, Aerts JMFG, Davies GJ Org Biomol Chem. 2022 Jan 26;20(4):877-886. doi: 10.1039/d1ob02287c. PMID:35015006<ref>PMID:35015006</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7op7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mannosidase 3D structures\|Mannosidase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacteroides thetaiotaomicron VPI-5482]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Aerts JMFG]]		[[Category: Aerts JMFG]]

OCA at 07:01, 2 February 2022

OCA — Wed, 02 Feb 2022 07:01:16 GMT

←Older revision		Revision as of 07:01, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7op7 is ~~ON HOLD until Paper Publication~~	+	==Bacteroides thetaiotaomicron mannosidase GH2 with beta-manno-configured N-alkyl cyclophellitol aziridine==
-		+	<StructureSection load='7op7' size='340' side='right'caption='[[7op7]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OP7 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7op7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7op7 OCA], [https://pdbe.org/7op7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7op7 RCSB], [https://www.ebi.ac.uk/pdbsum/7op7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7op7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Aerts JMFG]]
		+	[[Category: Armstrong Z]]
		+	[[Category: Beenakker TJM]]
		+	[[Category: Codee JDC]]
		+	[[Category: Davies GJ]]
		+	[[Category: Florea BI]]
		+	[[Category: Kuo C]]
		+	[[Category: McGregor NGS]]
		+	[[Category: Offen WA]]
		+	[[Category: Overkleeft HS]]
		+	[[Category: Wong C]]

OCA at 09:10, 4 August 2021

OCA — Wed, 04 Aug 2021 09:10:44 GMT

←Older revision		Revision as of 09:10, 4 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7op7 is ON HOLD	+	The entry 7op7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7op7" [edit=sysop:move=sysop]

OCA — Wed, 09 Jun 2021 11:14:15 GMT

Protected "7op7" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:14, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7op7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Jun 2021 11:14:14 GMT

New page: '''Unreleased structure''' The entry 7op7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7op7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7op7 - Revision history

OCA at 12:54, 1 February 2024

OCA at 07:01, 2 February 2022

OCA at 09:10, 4 August 2021

OCA: Protected "7op7" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7op7 is ON HOLD Authors: Description: Category: Unreleased Structures