7oxl - Revision history

OCA at 05:42, 8 September 2022

OCA — Thu, 08 Sep 2022 05:42:12 GMT

←Older revision		Revision as of 05:42, 8 September 2022
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	<StructureSection load='7oxl' size='340' side='right'caption='[[7oxl]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='7oxl' size='340' side='right'caption='[[7oxl]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7oxl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7oxl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6YU:FAD-MDL72527+adduct'>6YU</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6YU:FAD-MDL72527+adduct'>6YU</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxl OCA], [https://pdbe.org/7oxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxl RCSB], [https://www.ebi.ac.uk/pdbsum/7oxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxl OCA], [https://pdbe.org/7oxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxl RCSB], [https://www.ebi.ac.uk/pdbsum/7oxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SMOX_HUMAN SMOX_HUMAN]] Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.	+	[[https://www.uniprot.org/uniprot/SMOX_HUMAN SMOX_HUMAN]] Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Impagliazzo, A]]	+	[[Category: Impagliazzo A]]
-	[[Category: Johannsson, S]]	+	[[Category: Johannsson S]]
-	[[Category: Krapp, S]]	+	[[Category: Krapp S]]
-	[[Category: Thomsen, M]]	+	[[Category: Thomsen M]]
-	~~[[Category: Polyamine metabolism]]~~	+
-	~~[[Category: Spermidine]]~~	+
-	~~[[Category: Spermine]]~~	+
-	~~[[Category: Spermine oxidase]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 06:36, 18 August 2022

OCA — Thu, 18 Aug 2022 06:36:53 GMT

←Older revision		Revision as of 06:36, 18 August 2022
Line 1:		Line 1:

	==Crystal structure of human Spermine Oxidase==		==Crystal structure of human Spermine Oxidase==
-	<StructureSection load='7oxl' size='340' side='right'caption='[[7oxl]]' scene=''>	+	<StructureSection load='7oxl' size='340' side='right'caption='[[7oxl]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7oxl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxl OCA], [https://pdbe.org/7oxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxl RCSB], [https://www.ebi.ac.uk/pdbsum/7oxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxl ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6YU:FAD-MDL72527+adduct'>6YU</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxl OCA], [https://pdbe.org/7oxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxl RCSB], [https://www.ebi.ac.uk/pdbsum/7oxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/SMOX_HUMAN SMOX_HUMAN]] Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human spermine oxidase (hSMOX) plays a central role in polyamine catabolism. Due to its association with several pathological processes, including inflammation and cancer, hSMOX has garnered interest as a possible therapeutic target. Therefore, determination of the structure of hSMOX is an important step to enable drug discovery and validate hSMOX as a drug target. Using insights from hydrogen/deuterium exchange mass spectrometry (HDX-MS), we engineered a hSMOX construct to obtain the first crystal structure of hSMOX bound to the known polyamine oxidase inhibitor MDL72527 at 2.4 A resolution. While the overall fold of hSMOX is similar to its homolog, murine N1-acetylpolyamine oxidase (mPAOX), the two structures contain significant differences, notably in their substrate-binding domains and active site pockets. Subsequently, we employed a sensitive biochemical assay to conduct a high-throughput screen that identified a potent and selective hSMOX inhibitor, JNJ-1289. The co-crystal structure of hSMOX with JNJ-1289 was determined at 2.1 A resolution, revealing that JNJ-1289 binds to an allosteric site, providing JNJ-1289 with a high degree of selectivity towards hSMOX. These results provide crucial insights into understanding the substrate specificity and enzymatic mechanism of hSMOX, and for the design of highly selective inhibitors.
		+
		+	Structure of human spermine oxidase in complex with a highly selective allosteric inhibitor.,Diaz E, Adhikary S, Tepper AWJW, Riley D, Ortiz-Meoz R, Krosky D, Buyck C, Lamenca CM, Llaveria J, Fang L, Kalin JH, Klaren VNA, Fahmy S, Shaffer PL, Kirkpatrick R, Carbajo RJ, Thomsen M, Impagliazzo A Commun Biol. 2022 Aug 5;5(1):787. doi: 10.1038/s42003-022-03735-9. PMID:35931745<ref>PMID:35931745</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7oxl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Impagliazzo A]]	+	[[Category: Impagliazzo, A]]
-	[[Category: Johannsson S]]	+	[[Category: Johannsson, S]]
-	[[Category: Krapp S]]	+	[[Category: Krapp, S]]
-	[[Category: Thomsen M]]	+	[[Category: Thomsen, M]]
		+	[[Category: Polyamine metabolism]]
		+	[[Category: Spermidine]]
		+	[[Category: Spermine]]
		+	[[Category: Spermine oxidase]]
		+	[[Category: Unknown function]]

OCA at 05:13, 13 July 2022

OCA — Wed, 13 Jul 2022 05:13:52 GMT

←Older revision		Revision as of 05:13, 13 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7oxl is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of human Spermine Oxidase==
-		+	<StructureSection load='7oxl' size='340' side='right'caption='[[7oxl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OXL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oxl OCA], [https://pdbe.org/7oxl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oxl RCSB], [https://www.ebi.ac.uk/pdbsum/7oxl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oxl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Impagliazzo A]]
		+	[[Category: Johannsson S]]
		+	[[Category: Krapp S]]
		+	[[Category: Thomsen M]]

OCA at 09:10, 4 August 2021

OCA — Wed, 04 Aug 2021 09:10:48 GMT

←Older revision		Revision as of 09:10, 4 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7oxl is ON HOLD	+	The entry 7oxl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7oxl" [edit=sysop:move=sysop]

OCA — Fri, 02 Jul 2021 02:35:54 GMT

Protected "7oxl" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:35, 2 July 2021

OCA: New page: '''Unreleased structure''' The entry 7oxl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Fri, 02 Jul 2021 02:35:53 GMT

New page: '''Unreleased structure''' The entry 7oxl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7oxl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]