7p0x - Revision history

OCA at 12:59, 1 February 2024

OCA — Thu, 01 Feb 2024 12:59:39 GMT

←Older revision		Revision as of 12:59, 1 February 2024
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-	====	+	==Crystal structure of Thioredoxin reductase from Brugia Malayi==
-	<StructureSection load='7p0x' size='340' side='right'caption='[[7p0x]]' scene=''>	+	<StructureSection load='7p0x' size='340' side='right'caption='[[7p0x]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p0x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P0X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p0x OCA], [https://pdbe.org/7p0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p0x RCSB], [https://www.ebi.ac.uk/pdbsum/7p0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p0x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p0x OCA], [https://pdbe.org/7p0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p0x RCSB], [https://www.ebi.ac.uk/pdbsum/7p0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p0x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1I9G0T9_BRUMA A0A1I9G0T9_BRUMA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes in the thiol redox systems of microbial pathogens are promising targets for drug development. In this study we characterized the thioredoxin reductase (TrxR) selenoproteins from Brugia malayi and Onchocerca volvulus, filarial nematode parasites and causative agents of lymphatic filariasis and onchocerciasis, respectively. The two filarial enzymes showed similar turnover numbers and affinities for different thioredoxin (Trx) proteins, but with a clear preference for the autologous Trx. Human TrxR1 (hTrxR1) had a high and similar specific activity versus the human and filarial Trxs, suggesting that, in vivo, hTrxR1 could possibly be the reducing agent of parasite Trxs once they are released into the host. Both filarial TrxRs were efficiently inhibited by auranofin and by a recently described inhibitor of human TrxR1 (TRi-1), but not as efficiently by the alternative compound TRi-2. The enzyme from B. malayi was structurally characterized also in complex with NADPH and auranofin, producing the first crystallographic structure of a nematode TrxR. The protein represents an unusual fusion of a mammalian-type TrxR protein architecture with an N-terminal glutaredoxin-like (Grx) domain lacking typical Grx motifs. Unlike thioredoxin glutathione reductases (TGRs) found in platyhelminths and mammals, which are also Grx-TrxR domain fusion proteins, the TrxRs from the filarial nematodes lacked glutathione disulfide reductase and Grx activities. The structural determinations revealed that the Grx domain of TrxR from B. malayi contains a cysteine (C22), conserved in TrxRs from clade IIIc nematodes, that directly interacts with the C-terminal cysteine-selenocysteine motif of the homo-dimeric subunit. Interestingly, despite this finding we found that altering C22 by mutation to serine did not affect enzyme catalysis. Thus, although the function of the Grx domain in these filarial TrxRs remains to be determined, the results obtained provide insights on key properties of this important family of selenoprotein flavoenzymes that are potential drug targets for treatment of filariasis.
		+
		+	Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus.,Fata F, Gencheva R, Cheng Q, Lullo R, Ardini M, Silvestri I, Gabriele F, Ippoliti R, Bulman CA, Sakanari JA, Williams DL, Arner ESJ, Angelucci F Redox Biol. 2022 Mar 4;51:102278. doi: 10.1016/j.redox.2022.102278. PMID:35276442<ref>PMID:35276442</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p0x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioredoxin reductase 3D structures\|Thioredoxin reductase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brugia malayi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Ardini M]]
		+	[[Category: Arner ESJ]]
		+	[[Category: Cheng Q]]
		+	[[Category: Fata F]]
		+	[[Category: Gabriele F]]
		+	[[Category: Silvestri I]]
		+	[[Category: Williams DL]]

OCA at 10:24, 6 April 2022

OCA — Wed, 06 Apr 2022 10:24:17 GMT

←Older revision		Revision as of 10:24, 6 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7p0x ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7p0x' size='340' side='right'caption='[[7p0x]]' scene=''>
-	~~Authors~~: ~~Fata, F~~.~~, Ardini, M~~.~~, Silvestri, I~~.~~, Gabriele, F~~.~~, Cheng, Q~~.~~, Arner, E~~.~~S.J., Williams, D.L~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of Thioredoxin reductase from Brugia Malayi~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p0x OCA], [https://pdbe.org/7p0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p0x RCSB], [https://www.ebi.ac.uk/pdbsum/7p0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p0x ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Silvestri~~, ~~I]]~~	+	__TOC__
-	[~~[Category~~: ~~Williams, D~~.L]]	+	</StructureSection>
-	[~~[Category~~: ~~Cheng~~, ~~Q]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Fata~~, ~~F]]~~	+	[[Category: Z-disk]]
-	[~~[Category~~: ~~Arner, E~~.S.J]]	+
-	[[Category: ~~Gabriele, F~~]]	+
-	[[Category: ~~Ardini, M~~]]	+

OCA at 04:58, 18 August 2021

OCA — Wed, 18 Aug 2021 04:58:26 GMT

←Older revision		Revision as of 04:58, 18 August 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p0x is ON HOLD	+	The entry 7p0x is ON HOLD until Paper Publication

	Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.		Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.

OCA at 05:02, 11 August 2021

OCA — Wed, 11 Aug 2021 05:02:08 GMT

←Older revision		Revision as of 05:02, 11 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p0x is ON HOLD ~~until sometime in the future~~	+	The entry 7p0x is ON HOLD

	Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.		Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.
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	Description: Crystal structure of Thioredoxin reductase from Brugia Malayi		Description: Crystal structure of Thioredoxin reductase from Brugia Malayi
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Fata, F]]
-	[[Category: Gabriele, F]]
	[[Category: Silvestri, I]]		[[Category: Silvestri, I]]
	[[Category: Williams, D.L]]		[[Category: Williams, D.L]]
	[[Category: Cheng, Q]]		[[Category: Cheng, Q]]
		+	[[Category: Fata, F]]
	[[Category: Arner, E.S.J]]		[[Category: Arner, E.S.J]]
		+	[[Category: Gabriele, F]]
	[[Category: Ardini, M]]		[[Category: Ardini, M]]

OCA at 09:11, 4 August 2021

OCA — Wed, 04 Aug 2021 09:11:43 GMT

←Older revision		Revision as of 09:11, 4 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7p0x is ON HOLD	+	The entry 7p0x is ON HOLD until sometime in the future

	Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.		Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.
Line 7:		Line 7:
	Description: Crystal structure of Thioredoxin reductase from Brugia Malayi		Description: Crystal structure of Thioredoxin reductase from Brugia Malayi
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Fata, F]]
	[[Category: Gabriele, F]]		[[Category: Gabriele, F]]
		+	[[Category: Silvestri, I]]
	[[Category: Williams, D.L]]		[[Category: Williams, D.L]]
		+	[[Category: Cheng, Q]]
	[[Category: Arner, E.S.J]]		[[Category: Arner, E.S.J]]
	[[Category: Ardini, M]]		[[Category: Ardini, M]]
-	[[Category: Fata, F]]
-	[[Category: Silvestri, I]]
-	[[Category: Cheng, Q]]

OCA: Protected "7p0x" [edit=sysop:move=sysop]

OCA — Wed, 07 Jul 2021 08:54:59 GMT

Protected "7p0x" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 7 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p0x is ON HOLD Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L. Description: Crystal structure...

OCA — Wed, 07 Jul 2021 08:54:58 GMT

New page: '''Unreleased structure''' The entry 7p0x is ON HOLD Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L. Description: Crystal structure...

New page

'''Unreleased structure'''

The entry 7p0x is ON HOLD

Authors: Fata, F., Ardini, M., Silvestri, I., Gabriele, F., Cheng, Q., Arner, E.S.J., Williams, D.L.

Description: Crystal structure of Thioredoxin reductase from Brugia Malayi
[[Category: Unreleased Structures]]
[[Category: Gabriele, F]]
[[Category: Williams, D.L]]
[[Category: Arner, E.S.J]]
[[Category: Ardini, M]]
[[Category: Fata, F]]
[[Category: Silvestri, I]]
[[Category: Cheng, Q]]