OCA at 13:01, 1 February 2024

OCA — Thu, 01 Feb 2024 13:01:10 GMT

←Older revision		Revision as of 13:01, 1 February 2024
Line 1:		Line 1:

-	====	+	==Structure of the E3 ligase Smc5/Nse2 in complex with Ubc9-SUMO thioester mimetic==
-	<StructureSection load='7p47' size='340' side='right'caption='[[7p47]]' scene=''>	+	<StructureSection load='7p47' size='340' side='right'caption='[[7p47]], [[Resolution\|resolution]] 3.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p47]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P47 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p47 OCA], [https://pdbe.org/7p47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p47 RCSB], [https://www.ebi.ac.uk/pdbsum/7p47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p47 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.314Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p47 OCA], [https://pdbe.org/7p47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p47 RCSB], [https://www.ebi.ac.uk/pdbsum/7p47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p47 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SMC5_YEAST SMC5_YEAST] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Post-translational modification of proteins by ubiquitin and ubiquitin-like modifiers, such as SUMO, are key events in protein homeostasis or DNA damage response. Smc5/6 is a nuclear multi-subunit complex that participates in the recombinational DNA repair processes and is required in the maintenance of chromosome integrity. Nse2 is a subunit of the Smc5/6 complex that possesses SUMO E3 ligase activity by the presence of a SP-RING domain that activates the E2~SUMO thioester for discharge on the substrate. Here we present the crystal structure of the SUMO E3 ligase Nse2 in complex with an E2-SUMO thioester mimetic. In addition to the interface between the SP-RING domain and the E2, the complex reveals how two SIM (SUMO-Interacting Motif) -like motifs in Nse2 are restructured upon binding the donor and E2-backside SUMO during the E3-dependent discharge reaction. Both SIM interfaces are essential in the activity of Nse2 and are required to cope with DNA damage.
		+
		+	Structural basis for the E3 ligase activity enhancement of yeast Nse2 by SUMO-interacting motifs.,Varejao N, Lascorz J, Codina-Fabra J, Belli G, Borras-Gas H, Torres-Rosell J, Reverter D Nat Commun. 2021 Dec 1;12(1):7013. doi: 10.1038/s41467-021-27301-9. PMID:34853311<ref>PMID:34853311</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p47" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SUMO 3D Structures\|SUMO 3D Structures]]
		+	*[[SUMO conjugating enzyme Ubc9\|SUMO conjugating enzyme Ubc9]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Lascorz J]]
		+	[[Category: Reverter D]]
		+	[[Category: Varejao N]]

OCA at 12:37, 24 November 2021

OCA — Wed, 24 Nov 2021 12:37:06 GMT

←Older revision		Revision as of 12:37, 24 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7p47 ~~is ON HOLD until Jul 09 2023~~	+	====
-		+	<StructureSection load='7p47' size='340' side='right'caption='[[7p47]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p47 OCA], [https://pdbe.org/7p47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p47 RCSB], [https://www.ebi.ac.uk/pdbsum/7p47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p47 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7p47" [edit=sysop:move=sysop]

OCA — Wed, 21 Jul 2021 07:54:51 GMT

Protected "7p47" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:54, 21 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p47 is ON HOLD until Jul 09 2023 Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Jul 2021 07:54:50 GMT

New page: '''Unreleased structure''' The entry 7p47 is ON HOLD until Jul 09 2023 Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7p47 is ON HOLD until Jul 09 2023

Authors:

Description:
[[Category: Unreleased Structures]]

7p47 - Revision history

OCA at 13:01, 1 February 2024

OCA at 12:37, 24 November 2021

OCA: Protected "7p47" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7p47 is ON HOLD until Jul 09 2023 Authors: Description: Category: Unreleased Structures